Molecular cloning and functional characterization of NADPH-dependent cytochrome P450 reductase from the green microalga Botryococcus braunii, B race

Journal of Bioscience and Bioengineering

Volumn 125, Issue 1, 2018, Pages 30-37

  Tsou, Chung Yau ^a   Matsunaga, Shigeki ^a   Okada, Shigeru ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Botryococcus braunii; Cytochrome P450 reductase; Green microalga; NADPH; Oxidosqualene

Indexed keywords

AFFINITY CHROMATOGRAPHY; ALGAE; AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; PROTEINS; PURIFICATION; RECOMBINANT PROTEINS;

BOTRYOCOCCUS BRAUNII; CYTOCHROME P450 REDUCTASE; MICROALGA; NADPH; OXIDOSQUALENE;

ENZYME ACTIVITY;

AMINO ACID; COMPLEMENTARY DNA; CYTOCHROME C; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BOTRYOCOCCUS BRAUNII; CONTROLLED STUDY; DNA ISOLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PH; PROTEIN MOTIF; STEADY STATE; TEMPERATURE; ENZYMOLOGY; GENETICS; GREEN ALGA; METABOLISM; MICROALGA;

AMINO ACID SEQUENCE; CHLOROPHYTA; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYTOCHROMES C; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FLAVIN MONONUCLEOTIDE; FLAVIN-ADENINE DINUCLEOTIDE; HYDROGEN-ION CONCENTRATION; MICROALGAE; NADP; NADPH-FERRIHEMOPROTEIN REDUCTASE; OPEN READING FRAMES; TEMPERATURE;

EID: 85042314410     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2017.07.010     Document Type: Article

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