Quantifying gene expression in living cells with ratiometric bimolecular beacons

Methods in Molecular Biology

Volumn 1649, Issue , 2018, Pages 231-242

  Yang, Yantao ^a   Chen, Mingming ^a   Krueger, Christopher J ^a,b   Tsourkas, Andrew ^c   Chen, Antony K ^a  

^a PEKING UNIVERSITY   (China)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Molecular beacons; Quantifying gene expression; Ratiometric bimolecular beacons; RNA imaging; Single molecule detection; Single molecule fluorescence in situ hybridization

Indexed keywords

OLIGONUCLEOTIDE; SMALL INTERFERING RNA; MESSENGER RNA;

3' UNTRANSLATED REGION; CELL CULTURE; CENTRIFUGATION; CONTROLLED STUDY; ELECTROPORATION; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GEL FILTRATION; GENE EXPRESSION; HUMAN; HUMAN CELL; MOLECULAR BEACON; NUCLEAR EXPORT; RATIOMETRIC BIMOLECULAR BEACON; RNA ANALYSIS; RNA TRANSCRIPTION; SYNTHESIS; TANDEM REPEAT; ULTRAVIOLET SPECTROPHOTOMETRY; CELL LINE; CELL SURVIVAL; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR PROBE; PROCEDURES; THREE DIMENSIONAL IMAGING;

CELL LINE; CELL SURVIVAL; GENE EXPRESSION REGULATION; HUMANS; IMAGING, THREE-DIMENSIONAL; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR PROBES; RNA, MESSENGER;

EID: 85033700036     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-7213-5_15     Document Type: Chapter

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