메뉴 건너뛰기




Volumn 41, Issue 15, 2013, Pages

Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

EID: 84883485384     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkt561     Document Type: Article
Times cited : (24)

References (41)
  • 1
    • 79952638264 scopus 로고    scopus 로고
    • Cellular decision making and biological noise: From microbes to mammals
    • Balazsi,G., van Oudenaarden,A. and Collins,J.J. (2011) Cellular decision making and biological noise: from microbes to mammals. Cell, 144, 910-925
    • (2011) Cell , vol.144 , pp. 910-925
    • Balazsi, G.1    Van Oudenaarden, A.2    Collins, J.J.3
  • 2
  • 4
    • 53549123008 scopus 로고    scopus 로고
    • Nature, nurture, or chance: Stochastic gene expression and its consequences
    • Raj,A. and van Oudenaarden,A. (2008) Nature, nurture, or chance: stochastic gene expression and its consequences. Cell, 135, 216-226
    • (2008) Cell , vol.135 , pp. 216-226
    • Raj, A.1    Van Oudenaarden, A.2
  • 5
    • 67650319009 scopus 로고    scopus 로고
    • Single-molecule approaches to stochastic gene expression
    • Raj,A. and van Oudenaarden,A. (2009) Single-molecule approaches to stochastic gene expression. Annu. Rev. Biophys., 38, 255-270
    • (2009) Annu. Rev. Biophys , vol.38 , pp. 255-270
    • Raj, A.1    Van Oudenaarden, A.2
  • 6
    • 0032562720 scopus 로고    scopus 로고
    • Visualization of single RNA transcripts in situ
    • Femino,A.M., Fay,F.S., Fogarty,K. and Singer,R.H. (1998) Visualization of single RNA transcripts in situ. Science, 280, 585-590
    • (1998) Science , vol.280 , pp. 585-590
    • Femino, A.M.1    Fay, F.S.2    Fogarty, K.3    Singer, R.H.4
  • 9
    • 77249114260 scopus 로고    scopus 로고
    • Variability in gene expression underlies incomplete penetrance
    • Raj,A., Rifkin,S.A., Andersen,E. and van Oudenaarden,A. (2010) Variability in gene expression underlies incomplete penetrance. Nature, 463, 913-918
    • (2010) Nature , vol.463 , pp. 913-918
    • Raj, A.1    Rifkin, S.A.2    Andersen, E.3    Van Oudenaarden, A.4
  • 10
    • 84455200588 scopus 로고    scopus 로고
    • Single-molecule mRNA decay measurements reveal promoter-regulated mRNA stability in yeast
    • Trcek,T., Larson,D.R., Moldon,A., Query,C.C. and Singer,R.H. (2011) Single-molecule mRNA decay measurements reveal promoter-regulated mRNA stability in yeast. Cell, 147, 1484-1497
    • (2011) Cell , vol.147 , pp. 1484-1497
    • Trcek, T.1    Larson, D.R.2    Moldon, A.3    Query, C.C.4    Singer, R.H.5
  • 11
    • 33646591544 scopus 로고    scopus 로고
    • Transcriptional pulsing of a developmental gene
    • Chubb,J.R., Trcek,T., Shenoy,S.M. and Singer,R.H. (2006) Transcriptional pulsing of a developmental gene. Curr. Biol., 16, 1018-1025
    • (2006) Curr. Biol , vol.16 , pp. 1018-1025
    • Chubb, J.R.1    Trcek, T.2    Shenoy, S.M.3    Singer, R.H.4
  • 12
    • 28944434119 scopus 로고    scopus 로고
    • Realtime kinetics of gene activity in individual bacteria
    • Golding,I., Paulsson,J., Zawilski,S.M. and Cox,E.C. (2005) Realtime kinetics of gene activity in individual bacteria. Cell, 123, 1025-1036
    • (2005) Cell , vol.123 , pp. 1025-1036
    • Golding, I.1    Paulsson, J.2    Zawilski, S.M.3    Cox, E.C.4
  • 14
    • 71649113974 scopus 로고    scopus 로고
    • Visualization of individual scr mrnas during drosophila embryogenesis yields evidence for transcriptional bursting
    • Pare,A., Lemons,D., Kosman,D., Beaver,W., Freund,Y. and McGinnis,W. (2009) Visualization of individual Scr mRNAs during Drosophila embryogenesis yields evidence for transcriptional bursting. Curr. Biol., 19, 2037-2042
    • (2009) Curr. Biol , vol.19 , pp. 2037-2042
    • Pare, A.1    Lemons, D.2    Kosman, D.3    Beaver, W.4    Freund, Y.5    McGinnis, W.6
  • 15
    • 79955381895 scopus 로고    scopus 로고
    • Mammalian genes are transcribed with widely different bursting kinetics
    • Suter,D.M., Molina,N., Gatfield,D., Schneider,K., Schibler,U. and Naef,F. (2011) Mammalian genes are transcribed with widely different bursting kinetics. Science, 332, 472-474
    • (2011) Science , vol.332 , pp. 472-474
    • Suter, D.M.1    Molina, N.2    Gatfield, D.3    Schneider, K.4    Schibler, U.5    Naef, F.6
  • 16
    • 0021066596 scopus 로고
    • Localization of actin messenger RNA during early ascidian development
    • Jeffery,W.R., Tomlinson,C.R. and Brodeur,R.D. (1983) Localization of actin messenger RNA during early ascidian development. Dev. Biol., 99, 408-417
    • (1983) Dev. Biol , vol.99 , pp. 408-417
    • Jeffery, W.R.1    Tomlinson, C.R.2    Brodeur, R.D.3
  • 18
    • 66249103703 scopus 로고    scopus 로고
    • RNA granules: Posttranscriptional and epigenetic modulators of gene expression
    • Anderson,P. and Kedersha,N. (2009) RNA granules: posttranscriptional and epigenetic modulators of gene expression. Nat. Rev. Mol. Cell Biol., 10, 430-436
    • (2009) Nat. Rev. Mol. Cell Biol , vol.10 , pp. 430-436
    • Anderson, P.1    Kedersha, N.2
  • 19
    • 0031983834 scopus 로고    scopus 로고
    • Multicolor molecular beacons for allele discrimination
    • Tyagi,S., Bratu,D.P. and Kramer,F.R. (1998) Multicolor molecular beacons for allele discrimination. Nat. Biotechnol., 16, 49-53
    • (1998) Nat. Biotechnol , vol.16 , pp. 49-53
    • Tyagi, S.1    Bratu, D.P.2    Kramer, F.R.3
  • 20
    • 0029670262 scopus 로고    scopus 로고
    • Molecular beacons: Probes that fluoresce upon hybridization
    • Tyagi,S. and Kramer,F.R. (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol., 14, 303-308
    • (1996) Nat. Biotechnol , vol.14 , pp. 303-308
    • Tyagi, S.1    Kramer, F.R.2
  • 22
    • 27744463933 scopus 로고    scopus 로고
    • Stochasticity of manganese superoxide dismutase mRNA expression in breast carcinoma cells by molecular beacon imaging
    • Drake,T.J., Medley,C.D., Sen,A., Rogers,R.J. and Tan,W. (2005) Stochasticity of manganese superoxide dismutase mRNA expression in breast carcinoma cells by molecular beacon imaging. Chembiochem., 6, 2041-2047
    • (2005) Chembiochem , vol.6 , pp. 2041-2047
    • Drake, T.J.1    Medley, C.D.2    Sen, A.3    Rogers, R.J.4    Tan, W.5
  • 23
    • 56749169576 scopus 로고    scopus 로고
    • NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cells
    • Nitin,N. and Bao,G. (2008) NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cells. Bioconjug. Chem., 19, 2205-2211
    • (2008) Bioconjug. Chem , vol.19 , pp. 2205-2211
    • Nitin, N.1    Bao, G.2
  • 24
    • 65849507275 scopus 로고    scopus 로고
    • Simultaneous detection of mRNA and protein stem cell markers in live cells
    • Rhee,W.J. and Bao,G. (2009) Simultaneous detection of mRNA and protein stem cell markers in live cells. BMC Biotechnol., 9, 30
    • (2009) BMC Biotechnol , vol.9 , pp. 30
    • Rhee, W.J.1    Bao, G.2
  • 25
    • 34548724424 scopus 로고    scopus 로고
    • Avoiding falsepositive signals with nuclease-vulnerable molecular beacons in single living cells
    • Chen,A.K., Behlke,M.A. and Tsourkas,A. (2007) Avoiding falsepositive signals with nuclease-vulnerable molecular beacons in single living cells. Nucleic Acids Res., 35, e105
    • (2007) Nucleic Acids Res , vol.35
    • Chen, A.K.1    Behlke, M.A.2    Tsourkas, A.3
  • 26
    • 47249136607 scopus 로고    scopus 로고
    • Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA
    • Chen,A.K., Behlke,M.A. and Tsourkas,A. (2008) Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA. Nucleic Acids Res., 36, e69
    • (2008) Nucleic Acids Res , vol.36
    • Chen, A.K.1    Behlke, M.A.2    Tsourkas, A.3
  • 27
    • 75649136910 scopus 로고    scopus 로고
    • Sub-cellular trafficking and functionality of 20-O-methyl and 20-O-methylphosphorothioate molecular beacons
    • Chen,A.K., Behlke,M.A. and Tsourkas,A. (2009) Sub-cellular trafficking and functionality of 20-O-methyl and 20-O-methylphosphorothioate molecular beacons. Nucleic Acids Res., 37, e149
    • (2009) Nucleic Acids Res , vol.37
    • Chen, A.K.1    Behlke, M.A.2    Tsourkas, A.3
  • 28
    • 17144392600 scopus 로고    scopus 로고
    • TRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells
    • Mhlanga,M.M., Vargas,D.Y., Fung,C.W., Kramer,F.R. and Tyagi,S. (2005) tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells. Nucleic Acids Res., 33, 1902-1912
    • (2005) Nucleic Acids Res , vol.33 , pp. 1902-1912
    • Mhlanga, M.M.1    Vargas, D.Y.2    Fung, C.W.3    Kramer, F.R.4    Tyagi, S.5
  • 30
    • 78649565985 scopus 로고    scopus 로고
    • Imaging RNA in living cells with molecular beacons: Current perspective and challenges
    • Chen,A.K. and Tsourkas,A. (2009) Imaging RNA in living cells with molecular beacons: current perspective and challenges. J. Innov. Opt. Health Sci., 2, 315-324
    • (2009) J. Innov. Opt. Health Sci , vol.2 , pp. 315-324
    • Chen, A.K.1    Tsourkas, A.2
  • 31
    • 77955788881 scopus 로고    scopus 로고
    • Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells
    • Chen,A.K., Davydenko,O., Behlke,M.A. and Tsourkas,A. (2010) Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells. Nucleic Acids Res., 38, e148
    • (2010) Nucleic Acids Res , vol.38
    • Chen, A.K.1    Davydenko, O.2    Behlke, M.A.3    Tsourkas, A.4
  • 33
    • 0036798115 scopus 로고    scopus 로고
    • Structure-function relationships of shared-stem and conventional molecular beacons
    • Tsourkas,A., Behlke,M.A. and Bao,G. (2002) Structure-function relationships of shared-stem and conventional molecular beacons. Nucleic Acids Res., 30, 4208-4215
    • (2002) Nucleic Acids Res , vol.30 , pp. 4208-4215
    • Tsourkas, A.1    Behlke, M.A.2    Bao, G.3
  • 34
    • 26444477373 scopus 로고    scopus 로고
    • Automatic tracking of individual fluorescence particles: Application to the study of chromosome dynamics IEEE Trans
    • Sage,D., Neumann,F.R., Hediger,F., Gasser,S.M. and Unser,M. (2005) Automatic tracking of individual fluorescence particles: application to the study of chromosome dynamics IEEE Trans. Image Process., 14, 1372-1383
    • (2005) Image Process , vol.14 , pp. 1372-1383
    • Sage, D.1    Neumann, F.R.2    Hediger, F.3    Gasser, S.M.4    Unser, M.5
  • 35
    • 54749105886 scopus 로고    scopus 로고
    • Assessing the sensitivity of commercially available fluorophores to the intracellular environment
    • Chen,A.K., Cheng,Z., Behlke,M.A. and Tsourkas,A. (2008) Assessing the sensitivity of commercially available fluorophores to the intracellular environment. Anal. Chem., 80, 7437-7444
    • (2008) Anal. Chem , vol.80 , pp. 7437-7444
    • Chen, A.K.1    Cheng, Z.2    Behlke, M.A.3    Tsourkas, A.4
  • 36
    • 0033519623 scopus 로고    scopus 로고
    • Localization and anchoring of mRNA in budding yeast
    • Beach,D.L., Salmon,E.D. and Bloom,K. (1999) Localization and anchoring of mRNA in budding yeast. Curr. Biol., 9, 569-578
    • (1999) Curr. Biol , vol.9 , pp. 569-578
    • Beach, D.L.1    Salmon, E.D.2    Bloom, K.3
  • 38
    • 0038376482 scopus 로고    scopus 로고
    • Live imaging of endogenous rna reveals a diffusion and entrapment mechanism for nanos mrna localization in drosophila
    • Forrest,K.M. and Gavis,E.R. (2003) Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr. Biol., 13, 1159-1168
    • (2003) Curr. Biol , vol.13 , pp. 1159-1168
    • Forrest, K.M.1    Gavis, E.R.2
  • 40
    • 0034257926 scopus 로고    scopus 로고
    • CaMKIIalpha 30 untranslated region-directed mRNA translocation in living neurons: Visualization by GFP linkage
    • Rook,M.S., Lu,M. and Kosik,K.S. (2000) CaMKIIalpha 30 untranslated region-directed mRNA translocation in living neurons: visualization by GFP linkage. J. Neurosci., 20, 6385-6393
    • (2000) J. Neurosci , vol.20 , pp. 6385-6393
    • Rook, M.S.1    Lu, M.2    Kosik, K.S.3
  • 41
    • 33746492940 scopus 로고    scopus 로고
    • Localization of bicoid mRNA in late oocytes is maintained by continual active transport
    • T.T., Forrest,K.M. and Gavis,E.R. (2006) Localization of bicoid mRNA in late oocytes is maintained by continual active transport. Dev. Cell, 11, 251-262
    • (2006) Dev. Cell , vol.11 , pp. 251-262
    • Forrest, K.M.1    Gavis, E.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.