Fecal microbiota analysis of children with small intestinal bacterial overgrowth among residents of an urban slum in Brazil;Análise da microbiota fecal de crianças com sobrecrescimento bacteriano no intestino delgado de moradoras de uma favela urbana no Brasil

Jornal de Pediatria

Volumn 94, Issue 5, 2018, Pages 483-490

  Mello, Carolina Santos ^a   Rodrigues, Mirian Silva do Carmo ^a   Filho, Humberto Bezerra de Araújo ^a   Melli, Lígia Cristina Fonseca Lahoz ^a   Tahan, Soraia ^a   Pignatari, Antônio Carlos Campos ^a   de Morais, Mauro Batista ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Child; Environmental exposure; Fecal microbiota

Indexed keywords

HEMOGLOBIN; HYDROGEN; METHANE;

ANEMIA; ANTHROPOMETRIC PARAMETERS; ARTICLE; BACTERIAL OVERGROWTH; BACTERIUM; BACTEROIDES FRAGILIS; BACTEROIDETES; BIFIDOBACTERIUM; BRAZIL; CHILD; CLOSTRIDIUM PERFRINGENS; CROSS-SECTIONAL STUDY; ESCHERICHIA COLI; FECES MICROFLORA; FIRMICUTES; HEMOGLOBIN ANALYSIS; HEMOGLOBIN BLOOD LEVEL; HUMAN; HYDROGEN BREATH TEST; LACTOBACILLUS; MAJOR CLINICAL STUDY; METHANOBREVIBACTER SMITHII; PEPTOCLOSTRIDIUM DIFFICILE; POVERTY; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SMALL INTESTINE; STAPHYLOCOCCUS AUREUS; STUNTING; URBAN POPULATION; BLIND LOOP SYNDROME; BREATH ANALYSIS; COHORT ANALYSIS; COMPLICATION; FECES; FEMALE; GROWTH DISORDER; MALE; MICROBIOLOGY; PRESCHOOL CHILD;

ANEMIA; BLIND LOOP SYNDROME; BREATH TESTS; CHILD; CHILD, PRESCHOOL; COHORT STUDIES; CROSS-SECTIONAL STUDIES; FECES; FEMALE; GROWTH DISORDERS; HUMANS; INTESTINE, SMALL; MALE; POVERTY AREAS; REAL-TIME POLYMERASE CHAIN REACTION; URBAN POPULATION;

EID: 85033489044     PISSN: 00217557     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jped.2017.09.003     Document Type: Article

References (30)

1. Eckburg, P.B., Bik, E.M., Bernstein, C.N., Purdom, E., Dethlefsen, L., Sargent, M., et al. Diversity of the human intestinal microbial flora. Science 308 (2005), 1635–1638.
2. Boojiink, C.C., Zoetendal, E.G., Kleerebezem, M., de Vos, W.M., Microbial communities in the human small intestine: coupling diversity to metagenomics. Future Microbiol 2 (2007), 285–295.
3. Pyleris, E., Giamarellos-Bourboulis, E.J., Tzivras, D., Koussoulas, V., Barbatzas, C., Pimentel, M., The prevalence of overgrowth by aerobic bacteria in the small intestine by small bowel culture: relationship with irritable bowel syndrome. Dig Dis Sci 57 (2012), 1321–1329.
4. Crane, R.J., Jones, K.D., Berkley, J.A., Environmental enteric dysfunction: an overview. Food Nutr Bull 36 (2015), S76–S87.
5. Owino, V., Ahmed, T., Freemark, M., Kelly, P., Loy, A., Manary, M., et al. Environmental enteric dysfunction and growth failure/stunting in global child health. Pediatrics, 138, 2016, e20160641.
6. Guerrant, R.L., Leite, A.M., Pinkerton, R., Medeiros, P.H., Cavalcante, P.A., DeBoer, M., et al. Biomarkers of environmental enteropathy, inflammation, stunting, and impaired growth in children in Northeast Brazil. PLOS ONE, 11, 2016, e0158772.
7. Mello, C.S., Tahan, S., Melli, L.C., Rodrigues, M.S., de Mello, R.M., Scaletsky, I.C., et al. Methane production and small intestinal bacterial overgrowth in children living in a slum. World J Gastroenterol 18 (2012), 5932–5939.
8. Gasbarrini, A., Corazza, G.R., Gasbarrini, G., Montalto, M., Di Stefano, M., Basilisco, G., et al. Methodology and indications of H2-breath testing in gastrointestinal diseases: the Rome Consensus Conference. Aliment Pharmacol Ther 29 (2009), 1–49.
9. Mello, C.S., Rodrigues, M.S., Araújo-Filho, H.B., Melli, L.C., Tahan, S., Pignatari, A.C., et al. Gut microbiota differences in children from distinct socioeconomic levels living in the same urban area in Brazil. J Pediatr Gastroenterol Nutr 63 (2016), 460–465.
10. World Health Organization (WHO)., WHO Anthro Plus for personal computers manual: software for assessing growth of the world's children and adolescents. 2009, WHO, Geneva.
11. World Health Organization (WHO)., Iron deficiency anaemia – assessment, prevention and control. A guide for programme managers. 2001, WHO/NHD/01.3, Geneva.
12. Leiby, A., Mehta, D., Gopalareddy, V., Jackson-Walker, S., Horvath, K., Bacterial overgrowth and methane production in children with encopresis. J Pediatr 156 (2010), 766–770.
13. Nadkarni, M.A., Martin, F.E., Jacques, N.A., Hunter, N., Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148 (2002), 257–266.
14. Xu, P., Li, M., Zhang, J., Zhang, T., Correlation of intestinal microbiota with overweight and obesity in Kazakh school children. BMC Microbiol 12 (2012), 283–289.
15. Rinttilä, T., Kassinen, A., Malinen, E., Krogius, L., Palva, A., Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97 (2004), 1166–1177.
16. Rahn, K., De Grandis, S.A., Clarke, R.C., McEwen, S.A., Galán, J.E., Ginocchio, C., et al. Amplification o fan invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 6 (1992), 271–279.
17. Langendijk, P.S., Schut, F., Jansen, G.J., Raangs, G.C., Kamphuis, G.R., Wilkinson, M.H., et al. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol 61 (1995), 3069–3075.
18. Matsuki, T., Watanabe, K., Fujimoto, J., Miyamoto, Y., Takada, T., Matsumoto, K., et al. Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol 68 (2002), 5445–5451.
19. Huijsdens, X.W., Linskens, R.K., Mak, M., Meuwissen, S.G., Vandenbroucke-Grauls, C.M., Savelkoul, P.H., Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. J Clin Microbiol 40 (2002), 4423–4427.
20. Brakstad, O.G., Aasbakk, K., Maeland, J.A., Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol 30 (1992), 1654–1660.
21. Kato, N., Kim, S.M., Kato, H., Tanaka, K., Watanabe, K., Ueno, K., et al. Identification of enterotoxin-producing Clostridium perfringens by the polymerase chain reaction. Kansenshogaku Zasshi 67 (1993), 724–729.
22. Johnston, C., Ufnar, J.A., Griffith, J.F., Gooch, J.A., Stewart, J.R., A real-time qPCR assay for the detection of the nifH gene of Methanobrevibacter smithii, a potential indicator of sewage pollution. J Appl Microbiol 109 (2010), 1946–1956.
23. Nakagawa, T., Uemori, T., Asada, K., Kato, I., Harasawa, R., Acholeplasma laidlawii has tRNA genes in the 16S-23S spacer of the rRNA operon. J Bacteriol 174 (1992), 8163–8165.
24. Donowitz, J.R., Petri, W.A. Jr., Pediatric small intestinal bacterial overgrowth in low-income countries. Trends Mol Med 21 (2015), 6–15.
25. Rezania, S., Amirmozaffari, N., Tabarraei, B., Jeddi-Tehrani, M., Zarei, O., Alizadeh, R., et al. Extraction, purification and characterization of lipopolysaccharide from Escherichia coli and Salmonella typhi. Avicenna J Med Biotechnol 3 (2011), 3–9.
26. Lin, A., Bik, E.M., Costello, E.K., Dethlefsen, L., Haque, R., Relman, D.A., et al. Distinct distal gut microbiome diversity and composition in healthy children from Bangladesh and the United States. PLOS ONE, 8, 2013, e53838.
27. McCann, K.S., The diversity-stability debate. Nature 405 (2000), 228–233.
28. Campbell, R.K., Schulze, K.J., Shaikh, S., Mehra, S., Ali, H., Wu, L., et al. Biomarkers of environmental enteric dysfunction among children in rural Bangladesh. J Pediatr Gastroenterol Nutr 65 (2017), 40–46.
29. Donowitz, J.R., Haque, R., Kirkpatrick, B.D., Alam, M., Lu, M., Kabir, M., et al. Small intestine bacterial overgrowth and environmental enteropathy in Bangladeshi children. MBio 7 (2016), e02102–e2115.
30. Mello, C.S., Tahan, S., Melli, L.C., Rodrigues, M.S., Morais, M.B., Absorção intestinal de D-xilose e anemia em crianças escolares moradoras em um bolsão de pobreza (favela). Rev Chil Nutr, 36, 2009, 976.