The importance of matrix-assisted laser desorption ionization–time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar

Journal of Microbiology, Immunology and Infection

Volumn 50, Issue 5, 2017, Pages 723-726

  Chen, Jonathan H K ^a   Cheng, Vincent C C ^a   Wong, Oi Ying ^a   Wong, Sally C Y ^a   So, Simon Y C ^a   Yam, Wing Cheong ^a   Yuen, Kwok Yung ^a,b  

^a QUEEN MARY HOSPITAL   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

chromogenic agar; Clostridium difficile; matrix assisted laser desorption ionization time of flight

Indexed keywords

BACTERIAL RNA; RNA 16S; AGAR; CHROMOGENIC SUBSTRATE;

ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; CLOSTRIDIUM DISPORICUM; CLOSTRIDIUM HATHEWAYI; CLOSTRIDIUM TERTIUM; DIAGNOSTIC ACCURACY; GENE SEQUENCE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PEPTOCLOSTRIDIUM DIFFICILE; SPECIES IDENTIFICATION; CHEMISTRY; CLASSIFICATION; CLOSTRIDIUM INFECTION; COLOR; CULTURE MEDIUM; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PROCEDURES; REPRODUCIBILITY;

AGAR; BACTERIOLOGICAL TECHNIQUES; CHROMOGENIC COMPOUNDS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; COLOR; CULTURE MEDIA; HUMANS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 85032142405     PISSN: 16841182     EISSN: 19959133     Source Type: Journal    
DOI: 10.1016/j.jmii.2015.12.002     Document Type: Article

References (17)

1. Yuen, K.Y., Woo, P.C., Liang, R.H., Chiu, E.K., Chen, F.F., Wong, S.S., et al. Clinical significance of alimentary tract microbes in bone marrow transplant recipients. Diagn Microbiol Infect Dis 30 (1998), 75–81.
2. Bartlett, J.G., Moon, N., Chang, T.W., Taylor, N., Onderdonk, A.B., Role of Clostridium difficile in antibiotic-associated pseudomembranous colitis. Gastroenterology 75 (1978), 778–782.
3. Cheng, V.C., Yam, W.C., Chan, J.F., To, K.K., Ho, P.L., Yuen, K.Y., Clostridium difficile ribotype 027 arrives in Hong Kong. Int J Antimicrob Agents 34 (2009), 492–493.
4. Cheng, V.C., Yam, W.C., Lam, O.T., Tsang, J.L., Tse, E.Y., Siu, G.K., et al. Clostridium difficile isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong. Eur J Clin Microbiol Infect Dis 30 (2011), 1371–1381.
5. Hardy, K.J., Gossain, S., Thomlinson, D., Pillay, D.G., Hawkey, P.M., Reducing Clostridium difficile through early identification of clusters and the use of a standardised set of interventions. J Hosp Infect 75 (2010), 277–281.
6. Cheng, V.C., Chau, P.H., So, S.Y., Chen, J.H., Poon, R.W., Wong, S.C., et al. Containment of Clostridium difficile infection without reduction in antimicrobial use in Hong Kong. Eur J Clin Microbiol Infect Dis 34 (2015), 1381–1386.
7. Elsayed, S., Zhang, K., Human infection caused by Clostridium hathewayi. Emerg Infect Dis 10 (2004), 1950–1952.
8. Woo, P.C., Lau, S.K., Woo, G.K., Fung, A.M., Yiu, V.P., Yuen, K.Y., Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis. J Clin Microbiol 42 (2004), 5947–5949.
9. Eckert, C., Burghoffer, B., Lalande, V., Barbut, F., Evaluation of the chromogenic agar chromID C. difficile. J Clin Microbiol 51 (2013), 1002–1004.
10. Shin, B.M., Lee, E.J., Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens. Ann Lab Med 34 (2014), 15–19.
11. Carson, K.C., Boseiwaqa, L.V., Thean, S.K., Foster, N.F., Riley, T.V., Isolation of Clostridium difficile from faecal specimens—a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar. J Med Microbiol 62 (2013), 1423–1427.
12. Housman, S.T., Banevicius, M.A., Lamb, L.M., Nicolau, D.P., Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media. J Microbiol Immunol Infect 49:3 (2016), 445–447.
13. Yang, J.J., Nam, Y.S., Kim, M.J., Cho, S.Y., You, E., Soh, Y.S., et al. Evaluation of a chromogenic culture medium for the detection of Clostridium difficile. Yonsei Med J 55 (2014), 994–998.
14. Teng, J.L., Ho, T.C., Yeung, R.S., Wong, A.Y., Wang, H., Chen, C., et al. Evaluation of 16SpathDB 2.0, an automated 16S rRNA gene sequence database, using 689 complete bacterial genomes. Diagn Microbiol Infect Dis 78 (2014), 105–115.
15. Janda, J.M., Abbott, S.L., 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J Clin Microbiol 45 (2007), 2761–2764.
16. Perry, J.D., Asir, K., Halimi, D., Orenga, S., Dale, J., Payne, M., et al. Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours. J Clin Microbiol 48 (2010), 3852–3858.
17. Steer, T., Collins, M.D., Gibson, G.R., Hippe, H., Lawson, P.A., Clostridium hathewayi sp. nov., from human faeces. Syst Appl Microbiol 24 (2001), 353–357.