Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots

Journal of Virological Methods

Volumn 251, Issue , 2018, Pages 75-79

  Guichet, Emilande ^a   Serrano, Laetitia ^a   Laurent, Christian ^a   Eymard Duvernay, Sabrina ^a   Kuaban, Christopher ^b   Vidal, Laurent ^c   Delaporte, Eric ^a   Ngole, Eitel Mpoudi ^d   Ayouba, Ahidjo ^a   Peeters, Martine ^a  

^a INSERM   (France)

^b UNIVERSITY OF BAMENDA   (Cameroon)

^c AIX MARSEILLE UNIVERSITY   (France)

^d Institut de Recherche pour le Developpement Cameroon   (Cameroon)

Author keywords

Dried blood spot; HIV 1; Resource limited countries; RNA extraction; Viral load

Indexed keywords

DEOXYRIBONUCLEASE; VIRUS RNA;

ADULT; ANTIVIRAL THERAPY; ARTICLE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; COST; DRIED BLOOD SPOT TESTING; FREE VIRUS ELUTION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MEASUREMENT ACCURACY; MICROBIOLOGICAL PARAMETERS; PATIENT MONITORING; PRIORITY JOURNAL; RNA EXTRACTION; RNA ISOLATION; VIRUS LOAD; YOUNG ADULT; COMPARATIVE STUDY; EVALUATION STUDY; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; PLASMA; PROCEDURES; SPECIMEN HANDLING; VIROLOGY;

FALSE NEGATIVE REACTIONS; FALSE POSITIVE REACTIONS; HIV INFECTIONS; HIV-1; HUMANS; PLASMA; RNA, VIRAL; SPECIMEN HANDLING; VIRAL LOAD;

EID: 85031785348     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2017.10.014     Document Type: Article

References (25)

1. Aitken, S.C., Wallis, C.L., Stevens, W., de Wit, T.R., Schuurman, R., Stability of HIV-1 nucleic acids in dried blood spot samples for HIV-1 drug resistance genotyping. PLoS One, 10, 2015, e0131541.
2. Boillot, F., Serrano, L., Muwonga, J., Kabuayi, J.P., Kambale, A., Mutaka, F., Fujiwara, P.I., Decosas, J., Peeters, M., Delaporte, E., Implementation and operational research: programmatic feasibility of dried blood spots for the virological follow-up of patients on antiretroviral treatment in Nord Kivu, Democratic Republic of the Congo. J. Acquir. Immune Defic. Syndr. 71 (2016), e9–e15.
3. Etienne, L., Eymard-Duvernay, S., Aghokeng, A., Butel, C., Monleau, M., Peeters, M., Single real-time reverse transcription-PCR assay for detection and quantification of genetically diverse HIV-1, SIVcpz, and SIVgor strains. J. Clin. Microbiol. 51 (2013), 787–798.
4. Fajardo, E., Metcalf, C.A., Chaillet, P., Aleixo, L., Pannus, P., Panunzi, I., Triviño, L., Ellman, T., Likaka, A., Mwenda, R., Prospective evaluation of diagnostic accuracy of dried blood spots from finger prick samples for determination of HIV-1 load with the NucliSens Easy-Q HIV-1 version 2.0 assay in Malawi. J. Clin. Microbiol. 52 (2014), 1343–1351.
5. Goel, N., Ritchie, A.V., Mtapuri-Zinyowera, S., Zeh, C., Stepchenkova, T., Lehga, J., De Ruiter, A., Farleigh, L.E., Edemaga, D., So, R., Sembongi, H., Wisniewski, C., Nadala, L., Schito, M., Lee, H., Performance of the SAMBA I and II HIV-1 Semi-Q Tests for viral load monitoring at the point-of-care. J. Virol. Methods 244 (2017), 39–45.
6. ® HIV-1 viral load assay. PLoS One, 11, 2016, e0168244.
7. Guichet, E., Aghokeng, A., Eymard-Duvernay, S., Vidal, N., Ayouba, A., Mpoudi Ngole, E., Delaporte, E., Ciaffi, L., Peeters, M., Field evaluation of an open and polyvalent universal HIV-1/SIVcpz/SIVgor quantitative RT-PCR assay for HIV-1 viral load monitoring in comparison to Abbott RealTime HIV-1 in Cameroon. J. Virol. Methods 237 (2016), 121–126.
8. Inzaule, S.C., Hamers, R.L., Zeh, C.E., Rinke de Wit, T.F., Stringent HIV viral load threshold for virological failure using dried blood spots: is the perfect the enemy of the good?. J. Acquir. Immune Defic. Syndr. 71 (2016), e30–33.
9. MSF (Médecins sans Frontières), Making viral load routine. Successes and challenges in implementation of routine HIV viral load monitoring—Part 2: the viral load laboratory 2016., 2016 https://www.msfaccess.org/sites/default/files/MSF_assets/HIV_AIDS/Docs/AIDS_report_Part_2_MakingViralLoadRoutine_MSF_VL_Lab%20Report_Web_ENG_2016.pdf (Accessed 17 May 2017).
10. Makadzange, A.T., Boyd, F.K., Chimukangara, B., Masimirembwa, C., Katzenstein, D., Ndhlovu, C.E., A simple Phosphate-Buffered-Saline-Based extraction method improves specificity of HIV viral load monitoring using dried blood spots. J. Clin. Microbiol. 55 (2017), 2172–2179.
11. Mercier-Delarue, S., Vray, M., Plantier, J.C., Maillard, T., Adjout, Z., de Olivera, F., Schnepf, N., Maylin, S., Simon, F., Delaugerre, C., Higher specificity of nucleic acid sequence-based amplification isothermal technology than of real-time PCR for quantification of HIV-1 RNA on dried blood spots. J. Clin. Microbiol. 52 (2014), 52–56.
12. Monleau, M., Montavon, C., Laurent, C., Segondy, M., Montes, B., Delaporte, E., Boillot, F., Peeters, M., Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing. J. Clin. Microbiol. 47 (2009), 1107–1118.
13. Monleau, M., Aghokeng, A.F., Eymard-Duvernay, S., Dagnra, A., Kania, D., Ngo-Giang-Huong, N., Touré-Kane, C., Truong, L.X.T., Chaix, M.-L., Delaporte, E., Ayouba, A., Peeters, M., ANRS 12235 Study Group, Field evaluation of dried blood spots for routine HIV-1 viral load and drug resistance monitoring in patients receiving antiretroviral therapy in Africa and Asia. J. Clin. Microbiol. 52 (2014), 578–586.
14. Pannus, P., Claus, M., Gonzalez, M.M.P., Ford, N., Fransen, K., Sensitivity and specificity of dried blood spots for HIV-1 viral load quantification: a laboratory assessment of 3 commercial assays. Medicine (Baltimore), 95, 2016, e5475.
15. Parkin, N.T., Measurement of HIV-1 viral load for drug resistance surveillance using dried blood spots: literature review and modeling of contribution of DNA and RNA. AIDS Rev. 16 (2014), 160–171.
16. Roberts, T., Cohn, J., Bonner, K., Hargreaves, S., Scale-up of routine viral load testing in resource-poor settings: current and future implementation challenges. Clin. Infect. Dis. 62 (2016), 1043–1048.
17. Rouet, F., Kania, D., Tuaillon, E., Maiga, A., Nouhin, J., Rouzioux, C., Descamps, D., Plantier, J.C., Use of reverse transcription-PCR-based assays for quantification of HIV-1 in dried blood spots requires specific HIV-1 RNA isolation for monitoring of antiretroviral treatment efficiency. J. Clin. Microbiol. 52 (2014), 2740–2741.
18. Rutstein, S.E., Hosseinipour, M.C., Kamwendo, D., Soko, A., Mkandawire, M., Biddle, A.K., Miller, W.C., Weinberger, M., Wheeler, S.B., Sarr, A., Gupta, S., Mataya, R., Dried blood spots for viral load monitoring in Malawi: feasible and effective. PLoS One, 10, 2015, e0124748.
19. Taieb, F., Tram, T.H., Ho, H.T., Pham, V.A., Nguyen, L., Pham, B.H., Tong, L.A., Tuaillon, E., Delaporte, E., Nguyen, A.T., Bui, D.D., Do, N., Madec, Y., Evaluation of two techniques for viral load monitoring using dried blood spot in routine practice in vietnam (French national agency for AIDS and hepatitis research 12338). Open Forum Infect. Dis., 3, 2016, ofw142.
20. UNITAID, HIV/AIDS Diagnostics technology landscape. 5th edition, 2015 http://unitaid.org/images/marketdynamics/publications/UNITAID_HIV_Nov_2015_Dx_Landscape.PDF (Accessed 16 May 2017).
21. Viljoen, J., Gampini, S., Danaviah, S., Valéa, D., Pillay, S., Kania, D., Méda, N., Newell, M.-L., Van de Perre, P., Rouet, F., World Health Organization/ANRS 1289 Kesho Bora Study Group, Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso. J. Acquir. Immune Defic. Syndr. 55 (2010), 290–298.
22. WHO (World Health Organization), Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection. Recommendations for a public health approach. second edition, 2016 http://www.who.int/hiv/pub/arv/arv-2016/en/ (Accessed 16 May 2017).
23. Working Group on Modelling of Antiretroviral Therapy Monitoring Strategies in Sub-Saharan Africa, Phillips, A., Shroufi, A., Vojnov, L., Cohn, J., Roberts, T., Ellman, T., Bonner, K., Rousseau, C., Garnett, G., Cambiano, V., Nakagawa, F., Ford, D., Bansi-Matharu, L., Miners, A., Lundgren, J.D., Eaton, J.W., Parkes-Ratanshi, R., Katz, Z., Maman, D., Ford, N., Vitoria, M., Doherty, M., Dowdy, D., Nichols, B., Murtagh, M., Wareham, M., Palamountain, K.M., Chakanyuka Musanhu, C., Stevens, W., Katzenstein, D., Ciaranello, A., Barnabas, R., Braithwaite, R.S., Bendavid, E., Nathoo, K.J., van de Vijver, D., Wilson, D.P., Holmes, C., Bershteyn, A., Walker, S., Raizes, E., Jani, I., Nelson, L.J., Peeling, R., Terris-Prestholt, F., Murungu, J., Mutasa-Apollo, T., Hallett, T.B., Revill, P., Sustainable HIV treatment in Africa through viral-load-informed differentiated care. Nature 528 (2015), S68–76.
24. Wu, X., Crask, M., Ramirez, H., Landas, T., Do, T.D., Honisch, C., Will, S., Baum, P.D., A simple method to elute cell-free HIV from dried blood spots improves their usefulness for monitoring therapy. J. Clin. Virol. 65 (2015), 38–40.
25. Zida, S., Tuaillon, E., Barro, M., Kwimatouo Lekpa, A.F., Kagoné, T., Nacro, B., Abdoul Salam, O., Bolloré, K., Sanosyan, A., Plantier, J.-C., Meda, N., Sangaré, L., Rouzioux, C., Rouet, F., Kania, D., Estimation of HIV-1 DNA level interfering with the reliability of HIV-1 RNA quantification performed on dried blood spots (DBS) collected from successfully treated patients. J. Clin. Microbiol. 54 (2016), 1641–1643.