A simple phosphate-buffered-saline-based extraction method improves specificity of HIV viral load monitoring using dried blood spots

Journal of Clinical Microbiology

Volumn 55, Issue 7, 2017, Pages 2172-2179

  Makadzange, A Tariro ^a,b   Boyd, F Kathryn ^b   Chimukangar, Benjamin ^a,c   Masimirembw, Collen ^a,d   Katzenstein, David ^e   Ndhlovu, Chiratidzo E ^b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF ZIMBABWE   (Zimbabwe)

^c UNIVERSITY OF KWAZULU NATAL   (South Africa)

^d African Institute of Biomedical Science and Technology   (Zimbabwe)

^e STANFORD UNIVERSITY   (United States)

Author keywords

Cell free virus elution; DBS; Dried blood spots; Dried blood spots (DBS); HIV; Sub Saharan Africa; Viral load monitoring; Viral load quantification

Indexed keywords

ANTIRETROVIRUS AGENT; PHOSPHATE BUFFERED SALINE; BUFFER;

ADULT; ARTICLE; CELL-FREE VIRUS ELUTION METHOD; CHILD; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DRIED BLOOD SPOT TESTING; DRUG TREATMENT FAILURE; EXTRACTION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; LABORATORY DEVICE; LIMIT OF QUANTITATION; MAJOR CLINICAL STUDY; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PATIENT MONITORING; PLASMA; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VIREMIA; VIRUS LOAD; ZIMBABWE; ADOLESCENT; AFRICA SOUTH OF THE SAHARA; AGED; BLOOD; DESICCATION; EVALUATION STUDY; FEMALE; INFANT; MALE; MIDDLE AGED; NEWBORN; PRESCHOOL CHILD; PROCEDURES; SPECIMEN HANDLING; VERY ELDERLY; VIROLOGY; YOUNG ADULT;

ADOLESCENT; ADULT; AFRICA SOUTH OF THE SAHARA; AGED; AGED, 80 AND OVER; BLOOD; BUFFERS; CHILD; CHILD, PRESCHOOL; DESICCATION; FEMALE; HIV INFECTIONS; HUMANS; INFANT; INFANT, NEWBORN; MALE; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIRAL LOAD; YOUNG ADULT;

EID: 85021286961     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00176-17     Document Type: Article

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