Leukocyte Dynamics Influence Reference Gene Stability in Whole Blood: Data-Driven qRT-PCR Normalization Is a Robust Alternative for Measurement ofTranscriptional Biomarkers

Lab Medicine

Volumn 48, Issue 4, 2017, Pages 346-356

  O’Connell, Grant C ^a,b   Treadway, Madison B ^b   Petrone, Ashley B ^a   Tennant, Connie S ^a   Lucke Wold, Noelle ^a   Chantler, Paul D ^a,c   Barr, Taura L ^d  

^a WEST VIRGINIA UNIVERSITY   (United States)

^b WEST VIRGINIA UNIVERSITY   (United States)

^c WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d Valtari Bio Inc   (United States)

Author keywords

complete blood count; ddCT; delta delta CT; digital PCR; housekeeping gene; NORMA Gene; polymerase chain reaction; RNA; 2 microglobulin; actin; CT

Indexed keywords

ANTICOAGULANT AGENT; ANTITHROMBOCYTIC AGENT; BETA 2 MICROGLOBULIN; BETA ACTIN; BIOLOGICAL MARKER; CHOLESTEROL;

ADULT; AGED; ARTICLE; ATRIAL FIBRILLATION; BLOOD ANALYSIS; BLOOD CELL COUNT; CD4+ T LYMPHOCYTE; CEREBROVASCULAR ACCIDENT; CLINICAL ARTICLE; CONTROLLED STUDY; DIABETES MELLITUS; DIAGNOSTIC TEST ACCURACY STUDY; DIFFERENTIAL EXPRESSION ANALYSIS; DIGITAL POLYMERASE CHAIN REACTION; DYSLIPIDEMIA; FEMALE; GENETIC TRANSCRIPTION; HEART INFARCTION; HUMAN; HUMAN CELL; HYPERTENSION; INJURY SEVERITY; LEUKOCYTE; LEUKOCYTE COUNT; LYMPHOCYTE COUNT; MALE; MEASUREMENT; MICROARRAY ANALYSIS; NATIONAL INSTITUTES OF HEALTH STROKE SCALE; NEUTROPHIL COUNT; NEUTROPHIL LYMPHOCYTE RATIO; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SPECTROPHOTOMETRY; STROKE PATIENT; ALGORITHM; ESSENTIAL GENE; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; MIDDLE AGED; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; STANDARDS; VERY ELDERLY;

AGED; AGED, 80 AND OVER; ALGORITHMS; BIOMARKERS; FEMALE; GENE EXPRESSION PROFILING; GENES, ESSENTIAL; HUMANS; LEUKOCYTES; MALE; MIDDLE AGED; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 85031125466     PISSN: 00075027     EISSN: 19437730     Source Type: Journal    
DOI: 10.1093/LABMED/LMX035     Document Type: Article

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