MUC5AC and a Glycosylated Variant of MUC5B Alter Mucin Composition in Children With Acute Asthma

Chest

Volumn 152, Issue 4, 2017, Pages 771-779

  Welsh, Kathryn G ^a   Rousseau, Karine ^b   Fisher, Gemma ^a   Bonser, Luke R ^b   Bradding, Peter ^a   Brightling, Chris E ^a   Thornton, David J ^b   Gaillard, Erol A ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

airway obstruction; exacerbation; mucus; pediatric; sputum plug

Indexed keywords

MUCIN 5AC; MUCIN 5B; BIOLOGICAL MARKER; MUC5AC PROTEIN, HUMAN; MUC5B PROTEIN, HUMAN; MUCIN;

ADOLESCENT; ARTICLE; ASTHMA; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE EXACERBATION; ENZYME INHIBITION; FEMALE; FORCED EXPIRATORY VOLUME; HUMAN; IMMUNODETECTION; INFORMED CONSENT; LIMIT OF QUANTITATION; MALE; PRIORITY JOURNAL; PROTEIN FUNCTION; SPUTUM EXAMINATION; WESTERN BLOTTING; ACUTE DISEASE; CHEMISTRY; METABOLISM; MUCUS; PRESCHOOL CHILD; RESPIRATORY MUCOSA; SPUTUM;

ACUTE DISEASE; ADOLESCENT; ASTHMA; BIOMARKERS; BLOTTING, WESTERN; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; MALE; MUCIN 5AC; MUCIN-5B; MUCINS; MUCUS; RESPIRATORY MUCOSA; SPUTUM;

EID: 85030680502     PISSN: 00123692     EISSN: 19313543     Source Type: Journal    
DOI: 10.1016/j.chest.2017.07.001     Document Type: Article

References (39)

1. Royal College of Physicians. Why asthma still kills: the National Review of Asthma Deaths (NRAD) confidential enquiry report. Thorax 69:suppl 1 (2014), 1–192.
2. Sidebotham, H.J., Roche, W.R., Asthma deaths: persistent and preventable mortality. Histopathology 43:2 (2003), 105–117.
3. Rogers, D.F., Pulmonary mucus: pediatric perspective. Pediatr Pulmonol 36:3 (2003), 178–188.
4. Kuyper, L.M., Paré, P.D., Hogg, J.C., et al. Characterization of airway plugging in fatal asthma. Am J Med 115:1 (2003), 6–11.
5. Carroll, N.G., Mutavdzic, S., James, A.L., Increased mast cells and neutrophils in submucosal mucous glands and mucus plugging in patients with asthma. Thorax 57:8 (2002), 677–682.
6. Green, F.H., Williams, D.J., James, A., McPhee, L.J., Mitchell, I., Mauad, T., Increased myoepithelial cells of bronchial submucosal glands in fatal asthma. Thorax 65:1 (2010), 32–38.
7. Fahy, J.V., Goblet cell and mucin gene abnormalities in asthma. Chest 122:suppl (2002), 320S–326S.
8. Aikawa, T., Shimura, S., Sasaki, H., Ebina, M., Takishima, T., Marked goblet cell hyperplasia with mucus accumulation in the airways of patients who died of severe acute asthma attack. Chest 101:4 (1992), 916–921.
9. Jinnai, M., Niimi, A., Ueda, T., et al. Induced sputum concentrations of mucin in patients with asthma and chronic cough. Chest 137:5 (2010), 1122–1129.
10. Ordoñez, C.L., Khashayar, R., Wong, H.H., et al. Mild and moderate asthma is associated with airway goblet cell hyperplasia and abnormalities in mucin gene expression. Am J Respir Crit Care Med 163:2 (2001), 517–523.
11. Sheehan, J.K., Richardson, P.S., Fung, D.C., Howard, M., Thornton, D.J., Analysis of respiratory mucus glycoproteins in asthma: a detailed study from a patient who died in status asthmaticus. Am J Respir Cell Mol Biol 13:6 (1995), 748–756.
12. Groneberg, D.A., Eynott, P.R., Lim, S., et al. Expression of respiratory mucins in fatal status asthmaticus and mild asthma. Histopathology 40:4 (2002), 367–373.
13. Lachowicz-Scroggins, M.E., Yuan, S., Kerr, S.C., et al. Abnormalities in MUC5AC and MUC5B protein in airway mucus in asthma. Am J Respir Crit Care Med 194:10 (2016), 1296–1299.
14. Bonser, L.R., Zlock, L., Finkbeiner, W., Erle, D.J., Epithelial tethering of MUC5AC-rich mucus impairs mucociliary transport in asthma. J Clin Invest 126:6 (2016), 2367–2371.
15. Innes, A.L., Carrington, S.D., Thornton, D.J., et al. Ex vivo sputum analysis reveals impairment of protease-dependent mucus degradation by plasma proteins in acute asthma. Am J Respir Crit Care Med 180:3 (2009), 203–210.
16. Fahy, J.V., Dickey, B.F., Airway mucus function and dysfunction. N Engl J Med 363:23 (2010), 2233–2247.
17. Roy, M.G., Livraghi-Butrico, A., Fletcher, A.A., et al. Muc5b is required for airway defence. Nature 505:7483 (2014), 412–416.
18. Evans, C.M., Raclawska, D.S., Ttofali, F., et al. The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nat Commun, 6, 2015, 6281.
19. Kirkham, S., Kolsum, U., Rousseau, K., Singh, D., Vestbo, J., Thornton, D.J., MUC5B is the major mucin in the gel phase of sputum in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 178:10 (2008), 1033–1039.
20. Kirkham, S., Sheehan, J.K., Knight, D., Richardson, P.S., Thornton, D.J., Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B. Biochem J 361:pt 3 (2002), 537–546.
21. Thornton, D.J., Carlstedt, I., Howard, M., Devine, P.L., Price, M.R., Sheehan, J.K., Respiratory mucins: identification of core proteins and glycoforms. Biochem J 316:pt 3 (1996), 967–975.
22. Voynow, J.A., Rubin, B.K., Mucins, mucus, and sputum. Chest 135:2 (2009), 505–512.
23. Shimura, S., Sasaki, T., Sasaki, H., Takishima, T., Umeya, K., Viscoelastic properties of bronchorrhoea sputum in bronchial asthmatics. Biorheology 25:1-2 (1988), 173–179.
24. Messina, M.S., O'Riordan, T.G., Smaldone, G.C., Changes in mucociliary clearance during acute exacerbations of asthma. Am Rev Respir Dis 143:5 pt 1 (1991), 993–997.
25. Groneberg, D.A., Wagner, U., Chung, K.F., Mucus and fatal asthma [letter]. Am J Med 116:1 (2004), 66–67.
26. Cai, Y., Carty, K., Henry, R.L., Gibson, P.G., Persistence of sputum eosinophilia in children with controlled asthma when compared with healthy children. Eur Respir J 11:4 (1998), 848–853.
27. Pavord, I.D., Pizzichini, M.M., Pizzichini, E., Hargreave, F.E., The use of induced sputum to investigate airway inflammation. Thorax 52:6 (1997), 498–501.
28. Pellegrino, R., Viegi, G., Brusasco, V., et al. Interpretative strategies for lung function tests. Eur Respir J 26:5 (2005), 948–968.
29. Sheehan, J.K., Carlstedt, I., Size heterogeneity of human cervical mucus glycoproteins: studies performed with rate-zonal centrifugation and laser light-scattering. Biochem J 245:3 (1987), 757–762.
30. British Thoracic Society. British guideline on the management of asthma: a national clinical guideline. Thorax 69 (2014), 1–199.
31. Henderson, A.G., Ehre, C., Button, B., et al. Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure. J Clin Invest 124:7 (2014), 3047–3060.
32. Horsley, A., Rousseau, K., Ridley, C., et al. Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis. J Cyst Fibros 13:3 (2014), 260–266.
33. Liu, W., Luo, R., Chen, Y., et al. Interleukin-31 promotes helper T cell type-2 inflammation in children with allergic rhinitis. Pediatr Res 77:1-1 (2015), 20–28.
34. Parker, J., Sarlang, S., Thavagnanam, S., et al. A 3-D well-differentiated model of pediatric bronchial epithelium demonstrates unstimulated morphological differences between asthmatic and nonasthmatic cells. Pediatr Res 67:1 (2010), 17–22.
35. Burgel, P.R., Lazarus, S.C., Tam, D.C., et al. Human eosinophils induce mucin production in airway epithelial cells via epidermal growth factor receptor activation. J Immunol 167:10 (2001), 5948–5954.
36. Fischer, B.M., Voynow, J.A., Neutrophil elastase induces MUC5AC gene expression in airway epithelium via a pathway involving reactive oxygen species. Am J Respir Cell Mol Biol 26:4 (2002), 447–452.
37. Voynow, J.A., Young, L.R., Wang, Y., Horger, T., Rose, M.C., Fischer, B.M., Neutrophil elastase increases MUC5AC mRNA and protein expression in respiratory epithelial cells. Am J Physiol 276:5 pt 1 (1999), L835–L843.
38. Ordoñez, C.L., Shaughnessy, T.E., Matthay, M.A., Fahy, J.V., Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: clinical and biologic significance. Am J Respir Crit Care Med 161:4 pt 1 (2000), 1185–1190.
39. Dickey, B.F., Fahy, J.V., Kesimer, M., Boucher, R.C., Evans, C.M., Thornton, D., Measuring airway mucin 2 in patients with severe chronic obstructive pulmonary disease with bacterial colonization. Ann Am Thorac Soc 13:11 (2016), 2103–2104.