Methods to create a stringent selection system for mammalian cell lines

Cytotechnology

Volumn 63, Issue 4, 2011, Pages 371-384

  Van Blokland, H J M ^a   Hoeksema, F ^a   Siep, M ^a   Otte, A P ^a   Verhees, J A ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Error Prone PCR; Mammalian cells; Mutant selection markers; Recombinant gene expression; Selection marker; Zeocin

Indexed keywords

DNA; MARKER; UNCLASSIFIED DRUG; ZEOCIN;

ANIMAL CELL; ARTICLE; CELL LINE; CHO CELL; GENETIC SELECTION; MAMMAL CELL; MARKER GENE; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROCEDURES CONCERNING CELLS; PROTEIN EXPRESSION; STOP CODON; STRINGENT SELECTION SYSTEM;

MAMMALIA;

EID: 85027938896     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-011-9354-9     Document Type: Article

References (11)

1. FW Alt RE Kellems JF Bertino RT Schimke 1978 Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells J Biol Chem 253 1357 1370 1:CAS:528:DyaE1cXhtlCmsbc%3D (Pubitemid 8303433)
2. JD Bloom JJ Silberg CO Wilke DA Drummond C Adami FH Arnold 2005 Thermodynamic prediction of protein neutrality Proc Natl Acad Sci USA 102 606 611 10.1073/pnas.0406744102 1:CAS:528:DC%2BD2MXis1Cru7c%3D (Pubitemid 40282712)
3. SJ Child MK Miller AP Geballe 1999 Translational control by an upstream open reading frame in the HER-2/neu transcript J Biol Chem 274 24335 24341 10.1074/jbc.274.34.24335 1:CAS:528:DyaK1MXlsVOnu7Y%3D (Pubitemid 29390372)
4. RJ Kaufman PA Sharp 1982 Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase cDNA gene J Mol Biol 159 601 621 10.1016/0022-2836(82)90103-6 1:CAS:528:DyaL38XmtV2mtb8%3D (Pubitemid 13229017)
5. RJ Kaufman LC Wasley AJ Spiliotes SD Gossels SA Latt GR Larsen RM Kay 1985 Co amplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells Mol Cell Biol 5 1750 1759 1:CAS:528:DyaL2MXkvValu7o%3D
6. M Kozak 2001 Constraints on reinitiation of translation in mammals Nucleic Acids Res 29 5226 5232 10.1093/nar/29.24.5226 1:CAS:528: DC%2BD38Xmsleltg%3D%3D (Pubitemid 34065441)
7. SK Ng DIC Wang MGS Yap 2007 Application of destabilizing sequences on selection marker for improved recombinant protein productivity in CHO-DG44 Metab Eng 3 304 316 10.1016/j.ymben.2007.01.001 (Pubitemid 46823903)
8. H Niwa K Yamamura J Miyazaki 1991 Efficient selection for high-expression transfectants with a novel eukaryotic vector Gene 108 193 199 10.1016/0378-1119(91)90434-D 1:CAS:528:DyaK38Xlt1GksA%3D%3D
9. K Sautter B Enenkel 2005 Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity Biotechnol Bioeng 89 530 538 10.1002/bit.20374 1:CAS:528:DC%2BD2MXhvFGrsr0%3D (Pubitemid 40444807)
10. G Urlaub E Käs AM Carothers LA Chasin 1983 Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells Cell 33 405 412 10.1016/0092-8674(83)90422-1 1:CAS:528:DyaL3sXltVShs78%3D (Pubitemid 13062492)
11. HJM Van Blokland THJ Kwaks RGAB Sewalt JA Verhees VNA Klaren TK Siersma JWM Korse NC Teunissen S Botschuijver C Van Mer SY Man AP Otte 2007 A novel, high stringency selection system allows screening of few clones for high protein expression J Biotechnol 128 237 245 10.1016/j.jbiotec.2006.09.023 (Pubitemid 46074191)