Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes

Molecular Biotechnology

Volumn 50, Issue 3, 2012, Pages 221-228

  Sidoti, Francesca ^a   Bergallo, Massimiliano ^a   Terlizzi, Maria Elena ^a   Piasentin Alessio, Elsa ^a   Astegiano, Sara ^a   Gasparini, Giorgio ^b   Cavallo, Rossana ^a  

^a MOLINETTE HOSPITAL   (Italy)

^b bioMérieux Italia S p A   (Italy)

Author keywords

Human rhinovirus; Internal control; Molecular beacon; Nucleic acid sequence based amplification; RNA detection and quantification

Indexed keywords

DIAGNOSIS; FINANCE; PROBES; RNA;

HUMAN RHINOVIRUS; INTERNAL CONTROLS; MOLECULAR BEACON; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; RNA DETECTION;

BIOMOLECULES;

COMPLEMENTARY DNA; VIRUS DNA; VIRUS RNA; PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; HUMAN RHINOVIRUS; IN VITRO STUDY; LIMIT OF DETECTION; LUNG LAVAGE; MEASUREMENT ACCURACY; MEASUREMENT PRECISION; MEASUREMENT REPEATABILITY; MOLECULAR BEACON; MOLECULAR DIAGNOSIS; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; QUANTITATIVE ANALYSIS; REFERENCE VALUE; REPRODUCIBILITY; RHINOVIRUS INFECTION; VIRUS DETECTION; VIRUS ISOLATION; ADULT; AGED; CLASSIFICATION; FEMALE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MIDDLE AGED; REAL TIME POLYMERASE CHAIN REACTION; RHINOVIRUS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS;

ADULT; AGED; AGED, 80 AND OVER; DNA PRIMERS; FEMALE; HUMANS; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RHINOVIRUS; RNA, VIRAL; SELF-SUSTAINED SEQUENCE REPLICATION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, RNA; YOUNG ADULT;

EID: 85027916674     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9432-4     Document Type: Article

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