Rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology

Journal of Virological Methods

Volumn 146, Issue 1-2, 2007, Pages 29-35

  Deiman, B ^a   Schrover, C ^a   Moore, C ^b   Westmoreland, D ^b   van de Wiel, P ^a  

^a Wales Specialist Virology Centre   (Netherlands)

^b UNIVERSITY HOSPITAL OF WALES   (United Kingdom)

Author keywords

Amplification; Detection; NASBA; Real time; Respiratory; RSV

Indexed keywords

ANIMAL CELL; ARTICLE; ASPIRATION; CELL CULTURE; CONTROLLED STUDY; EQUIPMENT; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; LUNG LAVAGE; MOLECULAR BEACON; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY SYNCYTIAL PNEUMOVIRUS A; RESPIRATORY SYNCYTIAL PNEUMOVIRUS B; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; VIRUS DETECTION;

CROSS REACTIONS; HUMANS; NASOPHARYNX; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RNA, VIRAL; SELF-SUSTAINED SEQUENCE REPLICATION; SENSITIVITY AND SPECIFICITY;

RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS;

EID: 35648997987     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.05.030     Document Type: Article

References (17)

1. Abels S., Nadal D., Stroehle A., and Bossart W. Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management. J. Clin. Microbiol. 39 (2001) 3135-3139
2. Aldous W., Gerber K., Taggart E., Thomas J., Tidwell D., and Daly J. A comparison of Binax NOW to viral culture and direct fluorescent assay testing for respiratory syncytial virus. Diagn. Microbiol. Infect. Dis. 49 (2004) 265-268
3. Barenfanger J., Drake C., Leon N., Mueller T., and Troutt T. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J. Clin. Microbiol. 38 (2000) 2824-2828
4. Boivin G., Cote S., Dery P., De Serres G., and Bergeron M. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J. Clin. Microbiol. 42 (2004) 45-51
5. Deiman B., van Aarle P., and Sillekens P. Characteristics and applications of nucleic acid sequence-based amplification (NASBA). Mol. Biotechnol. 20 (2002) 163-179
6. van Deursen, P., Verhoeven, A., de Bie, P., van Grinsven, A., Borst, G., Jacobs, M., van de Wiel, P., 2004. Nucleic Acid Isolation Using the NucliSens Mini MAG Instrument. 14th ECCMID, Prague, poster presentation p. 832.
7. Dewhurst-Maridor G., Simonet V., Bornand J., Nicod L., and Pache P. Development of a quantitative TaqMan RT-PCR for respiratory syncytial virus. J. Virol. Methods 120 (2004) 41-49
8. Falsey A., Formica M., and Walsh E. Diagnosis of respiratory Syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness. J. Clin. Microbiol. 40 (2002) 817-820
9. Gruteke P., Glas A., Dierdorp M., Vreede W., Pilon J.-W., and Bruisten S. Practical implementation of a multiplex PCR for acute respiratory tract infections in children. J. Clin. Microbiol. 42 (2004) 5596-5603
10. Hu A., Colella M., Tam J., Rappaport R., and Cheng S.-M. Simulaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. J. Clin. Microbiol. 41 (2003) 149-154
11. Kuroiwa Y., Nagai K., Okita L., Ukae S., Mori T., Hotsubo T., and Tsutsumi H. Comparison of an immunochromatography test with multiplex reverse transcription-PCR for rapid diagnosis of respiratory syncytial virus infections. J. Clin. Microbiol 42 (2004) 4812-4814
12. Liolios L., Jenney A., Spelman D., Kotsimbos T., Catton M., and Wesselingh S. Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J. Clin. Microbiol. 39 (2001) 2779-2783
13. Lipson S. Rapid laboratory diagnostics during the winter respiratory virus season. J. Clin. Microbiol. 40 (2002) 733-734
14. Moore C., Valappil M., Cordon S., and Westmoreland D. Enhanced clinical utility of the NucliSens EasyQ RSV A + B Assay for rapid detection of respiratory syncytial virus in clinical samples. Eur. J. Clin. Microbiol. Infect. Dis. 25 (2006) 167-174
15. Ohm-Smith M., Nassos P., and Haller B. Evaluation of the Binax NOW, BD directigen, and BD directigen EZ assays for detection of respiratory syncytial virus. J. Clin. Microbiol. 42 (2004) 2996-2999
16. Templeton K., Scheltinga S.B.M., Kroes A., and Claas E. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by Influenza B viruses, respiratory syncytial virus and parainfluenza viruses 1, 2, 3 and 4. J. Clin. Microbiol. 42 (2004) 1564-1569
17. Whiley D., Syrmis W., Mackay I., and Sloots T. Detection of human respiratory syncytial virus in respiratory samples by light cycler reverse transcriptase PCR. J. Clin. Microbiol. 40 (2002) 4418-4422