Fusion of an alcohol dehydrogenase with an aminotransferase using a PAS linker to improve coupled enzymatic alcohol-to-amine conversion

Protein Engineering, Design and Selection

Volumn 29, Issue 12, 2016, Pages 557-562

  Lerchner, Alexandra ^a   Daake, Marina ^a   Jarasch, Alexander ^a   Skerra, Arne ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Active site; Chiral amine; Coupled assay; Fusion protein; Substrate channeling

Indexed keywords

ENZYMES; ESCHERICHIA COLI; RECOMBINANT PROTEINS;

ACTIVE SITE; ALCOHOL DEHYDROGENASE; CHIRAL AMINES; FUSION PROTEINS; PARACOCCUS DENITRIFICANS; SUBSTRATE CHANNELINGS; SUBSTRATE CONCENTRATIONS; TWO COMPONENT SYSTEMS;

SIZE EXCLUSION CHROMATOGRAPHY;

ALANINE; ALCOHOL; ALCOHOL DEHYDROGENASE; AMINE; AMINOTRANSFERASE; DIAMINE; FUSION PROTEIN; ISOSORBIDE; LEVODIONE REDUCTASE; OMEGA AMINOTRANSFERASE; OXIDOREDUCTASE; PROLINE; SERINE; UNCLASSIFIED DRUG; ALCOHOL DERIVATIVE; HYBRID PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIOCATALYSIS; CHEMICAL REACTION KINETICS; DIMERIZATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBUNIT; ESCHERICHIA COLI; HYDRODYNAMICS; LEIFSONIA; LEIFSONIA AQUATICA; MOLECULAR SIZE; NONHUMAN; PARACOCCUS DENITRIFICANS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; SIZE EXCLUSION CHROMATOGRAPHY; SUBSTRATE CONCENTRATION; TRANSAMINATION; ACTINOMYCETALES; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL; MUTATION; PROCEDURES; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN MULTIMERIZATION;

ACTINOMYCETALES; ALCOHOLS; AMINES; CATALYTIC DOMAIN; MODELS, MOLECULAR; MUTATION; OXIDOREDUCTASES; PARACOCCUS DENITRIFICANS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT FUSION PROTEINS; TRANSAMINASES;

EID: 85021389294     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzw039     Document Type: Article

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