Performance of a novel human immunodeficiency virus (HIV) type 1 total nucleic acid-based real-time PCR assay using whole blood and dried blood spots for diagnosis of HIV in infants

Journal of Clinical Microbiology

Volumn 46, Issue 12, 2008, Pages 3941-3945

  Stevens, Wendy ^a,b   Erasmus, Linda ^a   Moloi, Matsidisho ^a   Taleng, Thabo ^a   Sarang, Somaya ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b NONE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLOOD; CONTROLLED STUDY; DNA DETERMINATION; DNA ISOLATION; DNA VIRUS; EVALUATION RESEARCH; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NUCLEIC ACID ANALYSIS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SOUTH AFRICA;

BLOOD; CHILD; CHILD, PRESCHOOL; DESICCATION; DNA, VIRAL; HIV INFECTIONS; HIV-1; HUMANS; INFANT; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SOUTH AFRICA; SPECIMEN HANDLING;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 57349149113     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00754-08     Document Type: Article

References (26)

1. Bobat, R., D. Moodley, A. Coutsoudis, and H. Coovadia. 1997. Breastfeeding by HIV-1-infected women and outcome in their infants: a cohort study from Durban, South Africa. AIDS 11:1627-1633.
2. Brahmbhatt, H., G. Kigozi, F. Wabwire-Mangen, D. Serwadda, T. Lutalo, F. Nalugoda, N. Sewankambo, M. Kiduggavu, M. Wawer, and R. Gray. 2006. Mortality in HIV-infected and uninfected children of HIV-infected and uninfected mothers in rural Uganda. J. Acquir. Immune Defic. Syndr. 41:504-508.
3. Cassol, S., T. Salas, M. J. Gill, M. Montpetit, J. Rudnik, C. T. Sy, and M. V. O'Shaughnessy. 1992. Stability of dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction. J. Clin. Microbiol. 30:3039-3042.
4. Creek, T., A. Tanuri, M. Smith, K. Seipone, M. Smit, K. Legwaila, C. Motswere, M. Maruping, T. Nkoane, R. Ntumy, E. Bile, M. Mine, L. Lu, G. Tebele, L. Mazhani, M. K. Davis, T. H. Roels, P. H. Kilmarx, and N. Shaffer. 2008. Early diagnosis of human immunodeficiency virus in infants using polymerase chain reaction on dried blood spots in Botswana's national program for prevention of mother-to-child transmission. Pediatr. Infect. Dis. J. 27:22-26.
5. Curran, J. W., H. W. Jaffe, A. M. Hardy, W. M. Morgan, R. M. Selik, and T. J. Dondero. 1988. Epidemiology of HIV infection and AIDS in the United States. Science 239:610-616.
6. Driver, G. A., J. C. Patton, J. Moloi, W. S. Stevens, and G. G. Sherman. 2007. Low risk of contamination with automated and manual excision of dried blood spots for HIV DNA PCR testing in the routine laboratory. J. Virol. Methods 146:397-400.
7. Fischer, A., C. Lejczak, C. Lambert, J. Servais, N. Makombe, J. Rusine, T. Staub, R. Hemmer, F. Schneider, J. C. Schmit, and V. Arendt. 2004. Simple DNA extraction method for dried blood spots and comparison of two PCR assays for diagnosis of vertical human immunodeficiency virus type 1 transmission in Rwanda. J. Clin. Microbiol. 42:16-20.
8. Joint United Nations Programme on HIV/AIDS and World Health Organization. 2007. AIDS epidemic update, December 2007. Joint United Nations Programme on HIV/AIDS (UNAIDS) and World Health Organization (WHO), Geneva, Switzerland. http://www.unaids.org/en/KnowledgeCentre/HIVData/EpiUpdate/ EpiUpdArchive/2007/.
9. Lambert, J. S., D. R. Harris, E. R. Stiehm, J. Moye, Jr., M. G. Fowler, W. A. Meyer, III, J. Bethel, and L. M. Mofenson. 2003. Performance characteristics of HIV-1 culture and HIV-1 DNA and RNA amplification assays for early diagnosis of perinatal HIV-1 infection. J. Acquir. Immune Defic. Syndr. 34:512-519.
10. Lehman, D. A., and C. Farquhar. 2007. Biological mechanisms of vertical human immunodeficiency virus (HIV-1) transmission. Rev. Med. Virol. 17:381-403.
11. Luo, W., H. Yang, K. Rathbun, C. P. Pau, and C. Y. Ou. 2005. Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay. J. Clin. Microbiol. 43:1851-1857.
12. National Department of Health, South Africa. 2004. National antiretroviral treatment guidelines, 1st ed. National Department of Health, Pretoria, South Africa. http://www.doh.gov.za/docs/factsheets/guidelines/ artguide04-f.html.
13. National Institute of Allergy and Infectious Diseases. 2007. Questions and answers. Children with HIV early antiretroviral therapy (CHER) study: treating HIV-infected infants early helps them live longer. South African clinical trial modified because of initial data. National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD. http://www3.niaid.nih.gov/news/QA/CHER_QA.htm.
14. Nduati, R., G. John, D. Mbori-Ngacha, B. Richardson, J. Overbaugh, A. Mwatha, J. Ndinya-Achola, J. Bwayo, F. E. Onyango, J. Hughes, and J. Kreiss. 2000. Effect of breastfeeding and formula feeding on transmission of HIV-1: a randomized clinical trial. JAMA 283:1167-1174.
15. Newell, M. L., H. Coovadia, M. Cortina-Borja, N. Rollins, P. Gaillard, and F. Dabis. 2004. Mortality of infected and uninfected infants born to HIV-infected mothers in Africa: a pooled analysis. Lancet 364:1236-1243.
16. Nyambi, P. N., K. Fransen, H. De Beenhouwer, E. N. Chomba, M. Temmerman, J. O. Ndinya-Achola, P. Piot, and G. van der Groen. 1994. Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers. J. Clin. Microbiol. 32:2858-2860.
17. Ou, C. Y., H. Yang, S. Balinandi, S. Sawadogo, V. Shanmugam, P. M. Tih, C. Adje-Toure, S. Tancho, L. K. Ya, M. Bulterys, R. Downing, and J. N. Nkengasong. 2007. Identification of HIV-1 infected infants and young children using real-time RT PCR and dried blood spots from Uganda and Cameroon. J. Virol. Methods 144:109-114.
18. Patton, J. C., E. Akkers, A. H. Coovadia, T. M. Meyers, W. S. Stevens, and G. G. Sherman. 2007. Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory. Clin. Vaccine Immunol. 14:201-203.
19. Reisler, R. B., D. M. Thea, V. Pliner, T. Green, F. Lee, S. Nesheim, T. Brown, M. Kalish, T. M. Folks, and W. Heneine. 2001. Early detection of reverse transcriptase activity in plasma of neonates infected with HIV-1: a comparative analysis with RNA-based and DNA-based testing using polymerase chain reaction. J. Acquir. Immune Defic. Syndr. 26:93-102.
20. Rouet, F., C. Sakarovitch, P. Msellati, N. Elenga, C. Montcho, I. Viho, S. Blanche, C. Rouzioux, F. Dabis, and V. Leroy. 2003. Pediatric viral human immunodeficiency virus type 1 RNA levels, timing of infection, and disease progression in African HIV-1-infected children. Pediatrics 112:e289.
21. Schumacher, W., E. Frick, M. Kauselmann, V. Maier-Hoyle, R. van der Vliet, and R. Babiel. 2007. Fully automated quantification of human immunodeficiency virus (HIV) type 1 RNA in human plasma by the COBAS AmpliPrep/COBAS TaqMan system. J. Clin. Virol. 38:304-312.
22. Sherman, G., P. Cooper, A. Coovadia, A. Puren, S. Jones, M. Mokhachane, and K. Bolton. 2005. Polymerase chain reaction for diagnosis of human immunodeficiency virus infection in infancy in low resource settings. Pediatr. Infect. Dis. J. 24:993-997.
23. Sherman, G. G., G. Stevens, S. A. Jones, P. Horsfield, and W. S. Stevens. 2005. Dried blood spots improve access to HIV diagnosis and care for infants in low-resource settings. J. Acquir. Immune Defic. Syndr. 38:615-617.
24. Steketee, R. W., E. J. Abrams, D. M. Thea, T. M. Brown, G. Lambert, S. Orloff, J. Weedon, M. Bamji, E. E. Schoenbaum, J. Rapier, M. L. Kalish, et al. 1997. Early detection of perinatal human immunodeficiency virus (HIV) type 1 infection using HIV RNA amplification and detection. J. Infect. Dis. 175:707-711.
25. Stevens, W., G. Sherman, R. Downing, L. Parsons, C. Ou, S. Crowley, G. Gershy-Damet, K. Fransen, M. Bulterys, J. Homsy, L. Lu, T. Finkbeiner, and J. Nkengasong. 2008. Role of the laboratory in ensuring global access to ARV treatment for HIV-infected children: consensus statement on the performance of laboratory assays for early infant diagnosis. Open AIDS J. 2:17-25.
26. Varga, C., G. Sherman, J. Maphosa, and S. Jones. 2005. Psychosocial consequences of early diagnosis of HIV status in vertically exposed infants in Johannesburg, South Africa. Health Care Women Int. 26:387-397.