-
1
-
-
84964313213
-
Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans
-
Arribere, J.A., Bell, R.T., Fu, B.X., Artiles, K.L., Hartman, P.S., and Fire, A.Z. 2014. Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics 198:837-846. doi: 10.1534/genetics.114.169730.
-
(2014)
Genetics
, vol.198
, pp. 837-846
-
-
Arribere, J.A.1
Bell, R.T.2
Fu, B.X.3
Artiles, K.L.4
Hartman, P.S.5
Fire, A.Z.6
-
2
-
-
34047118522
-
CRISPR provides acquired resistance against viruses in prokaryotes
-
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D.A., and Horvath, P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science 315:1709-1712. doi: 10.1126/science.1138140.
-
(2007)
Science
, vol.315
, pp. 1709-1712
-
-
Barrangou, R.1
Fremaux, C.2
Deveau, H.3
Richards, M.4
Boyaval, P.5
Moineau, S.6
Romero, D.A.7
Horvath, P.8
-
3
-
-
79953250082
-
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
-
Deltcheva, E., Chylinski, K., Sharma, C.M., Gonzales, K., Chao, Y., Pirzada, Z.A., Eckert, M.R., Vogel, J., and Charpentier, E. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471:602-607. doi: 10.1038/nature09886.
-
(2011)
Nature
, vol.471
, pp. 602-607
-
-
Deltcheva, E.1
Chylinski, K.2
Sharma, C.M.3
Gonzales, K.4
Chao, Y.5
Pirzada, Z.A.6
Eckert, M.R.7
Vogel, J.8
Charpentier, E.9
-
4
-
-
84884904381
-
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
-
Dickinson, D.J., Ward, J.D., Reiner, D.J., and Goldstein, B. 2013. Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat. Methods 10:1028-1034. doi: 10.1038/nmeth.2641.
-
(2013)
Nat. Methods
, vol.10
, pp. 1028-1034
-
-
Dickinson, D.J.1
Ward, J.D.2
Reiner, D.J.3
Goldstein, B.4
-
5
-
-
84939433336
-
Streamlined genome engineering with a self-excising drug selection cassette
-
Dickinson, D.J., Pani, A.M., Heppert, J.K., Higgins, C.D., and Goldstein, B. 2015. Streamlined genome engineering with a self-excising drug selection cassette. Genetics 200:1035-1049. doi: 10.1534/genetics.115.178335.
-
(2015)
Genetics
, vol.200
, pp. 1035-1049
-
-
Dickinson, D.J.1
Pani, A.M.2
Heppert, J.K.3
Higgins, C.D.4
Goldstein, B.5
-
6
-
-
15444367003
-
Media preparation and bacteriological tools
-
Elbing, K. and Brent, R. 2002. Media preparation and bacteriological tools. Curr. Protoc. Mol. Biol. 59:1.1.1-1.1.7.
-
(2002)
Curr. Protoc. Mol. Biol.
, vol.59
, pp. 1.1.1-1.1.7
-
-
Elbing, K.1
Brent, R.2
-
7
-
-
84926433352
-
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design
-
Farboud, B. and Meyer, B.J. 2015. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics 199:959-971. doi: 10.1534/genetics.115.175166.
-
(2015)
Genetics
, vol.199
, pp. 959-971
-
-
Farboud, B.1
Meyer, B.J.2
-
8
-
-
84881475586
-
Heritable genome editing in C
-
Friedland, A.E., Tzur, Y.B., Esvelt, K.M., Colaiacovo, M.P., Church, G.M., and Calarco, J.A. 2013. Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat. Methods 10:741-743. doi: 10.1038/nmeth.2532.
-
(2013)
elegans via a CRISPR-Cas9 system. Nat. Methods
, vol.10
, pp. 741-743
-
-
Friedland, A.E.1
Tzur, Y.B.2
Esvelt, K.M.3
Colaiacovo, M.P.4
Church, G.M.5
Calarco, J.A.6
-
9
-
-
84856470784
-
Improved Mos1-mediated transgenesis in C
-
Frokjaer-Jensen, C., Davis, M.W., Ailion, M., and Jorgensen, E.M. 2012. Improved Mos1-mediated transgenesis in C. elegans. Nat. Methods 9:117-118. doi: 10.1038/nmeth.1865.
-
(2012)
elegans. Nat. Methods
, vol.9
, pp. 117-118
-
-
Frokjaer-Jensen, C.1
Davis, M.W.2
Ailion, M.3
Jorgensen, E.M.4
-
10
-
-
78149261827
-
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
-
Garneau, J.E., Dupuis, M.E., Villion, M., Romero, D.A., Barrangou, R., Boyaval, P., Fremaux, C., Horvath, P., Magadan, A.H., and Moineau, S. 2010. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67-71. doi: 10.1038/nature09523.
-
(2010)
Nature
, vol.468
, pp. 67-71
-
-
Garneau, J.E.1
Dupuis, M.E.2
Villion, M.3
Romero, D.A.4
Barrangou, R.5
Boyaval, P.6
Fremaux, C.7
Horvath, P.8
Magadan, A.H.9
Moineau, S.10
-
11
-
-
74249095519
-
Single Worm PC
-
He, F. 2011. Single Worm PCR. Bio. Protoc. e60. Available at http://www.bio-protocol.org/e60. Horvath, P. and Barrangou, R. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science 327:167-170. doi: 10.1126/science.1179555.
-
(2011)
Bio. Protoc. e60. Available at http://www.bio-protocol.org/e60. Horvath, P. and Barrangou, R. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science
, vol.327
, pp. 167-170
-
-
He, F.1
-
12
-
-
84865070369
-
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
-
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., and Charpentier, E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816-821. doi: 10.1126/science.1225829.
-
(2012)
Science
, vol.337
, pp. 816-821
-
-
Jinek, M.1
Chylinski, K.2
Fonfara, I.3
Hauer, M.4
Doudna, J.A.5
Charpentier, E.6
-
13
-
-
85019535679
-
DNA damage sensitivity assays in Caenorhabditis elegans
-
Kim, H.M. and Colaiacovo, M.P. 2015a. DNA damage sensitivity assays in Caenorhabditis elegans. Bio. Protoc. 5:e1487.
-
(2015)
Bio. Protoc
, vol.5
-
-
Kim, H.M.1
Colaiacovo, M.P.2
-
14
-
-
84931311813
-
New insights into the post-translational regulation of DNA damage response and doublestrand break repair in Caenorhabditis elegans
-
Kim, H.M. and Colaiacovo, M.P. 2015b. New insights into the post-translational regulation of DNA damage response and doublestrand break repair in Caenorhabditis elegans. Genetics 200:495-504. doi: 10.1534/genetics.115.175661.
-
(2015)
Genetics
, vol.200
, pp. 495-504
-
-
Kim, H.M.1
Colaiacovo, M.P.2
-
15
-
-
84905665662
-
A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans
-
Kim, H., Ishidate, T., Ghanta, K.S., Seth, M., Conte, D., Jr., Shirayama, M., andMello, C.C. 2014. A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Genetics 197:1069-1080. doi: 10.1534/genetics.114.166389.
-
(2014)
Genetics
, vol.197
, pp. 1069-1080
-
-
Kim, H.1
Ishidate, T.2
Ghanta, K.S.3
Seth, M.4
Conte, D.5
Shirayama, M.6
Mello, C.C.7
-
16
-
-
7144230803
-
Enzymatic amplification of DNA by PCR: Standard procedures and optimization
-
Kramer, M. F. and Coen, D. M. 2000. Enzymatic amplification of DNA by PCR: Standard procedures and optimization. Curr. Protoc. Mol. Biol. 56:15.1.1-15.1.14.
-
(2000)
Curr. Protoc. Mol. Biol.
, vol.56
, pp. 15.1.1-15.1.14
-
-
Kramer, M.F.1
Coen, D.M.2
-
17
-
-
84873734105
-
RNA-guided human genome engineering via Cas9
-
Mali, P., Yang, L., Esvelt, K.M., Aach, J., Guell, M., DiCarlo, J.E., Norville, J.E., and Church, G.M. 2013. RNA-guided human genome engineering via Cas9. Science 339:823-826. doi: 10.1126/science.1232033.
-
(2013)
Science
, vol.339
, pp. 823-826
-
-
Mali, P.1
Yang, L.2
Esvelt, K.M.3
Aach, J.4
Guell, M.5
DiCarlo, J.E.6
Norville, J.E.7
Church, G.M.8
-
18
-
-
84943592701
-
Efficient genome editing in Caenorhabditis elegans with a toolkit of dual-marker selection cassettes
-
Norris, A.D., Kim, H.M., Colaiacovo, M.P., and Calarco, J.A. 2015a. Efficient genome editing in Caenorhabditis elegans with a toolkit of dual-marker selection cassettes. Genetics 201:449-458. doi: 10.1534/genetics.115.180679.
-
(2015)
Genetics
, vol.201
, pp. 449-458
-
-
Norris, A.D.1
Kim, H.M.2
Colaiacovo, M.P.3
Calarco, J.A.4
-
19
-
-
84943592701
-
Efficient genome editing in Caenorhabditis elegans with a toolkit of dual marker selection cassettes
-
Norris, A.D., Kim, H.M., Colaiacovo, M.P., and Calarco, J.A. 2015b. Efficient genome editing in Caenorhabditis elegans with a toolkit of dual marker selection cassettes. Genetics 201:449-458. doi: 10.1534/genetics.115.180679.
-
(2015)
Genetics
, vol.201
, pp. 449-458
-
-
Norris, A.D.1
Kim, H.M.2
Colaiacovo, M.P.3
Calarco, J.A.4
-
20
-
-
84915746762
-
Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans
-
Paix, A., Wang, Y., Smith, H.E., Lee, C.Y., Calidas, D., Lu, T., Smith, J., Schmidt, H., Krause, M.W., and Seydoux, G. 2014. Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics 198:1347-1356. doi: 10.1534/genetics.114.170423.
-
(2014)
Genetics
, vol.198
, pp. 1347-1356
-
-
Paix, A.1
Wang, Y.2
Smith, H.E.3
Lee, C.Y.4
Calidas, D.5
Lu, T.6
Smith, J.7
Schmidt, H.8
Krause, M.W.9
Seydoux, G.10
-
21
-
-
0000648512
-
Introduction of plasmid DNA into cells
-
Seidman, C. E., Struhl, K., Sheen, J., and Jessen, T. 1997. Introduction of plasmid DNA into cells. Curr. Protoc. Mol. Biol. 37:1.8.1-1.8.10.
-
(1997)
Curr. Protoc. Mol. Biol.
, vol.37
, pp. 1.8.1-1.8.10
-
-
Seidman, C.E.1
Struhl, K.2
Sheen, J.3
Jessen, T.4
-
22
-
-
38449085292
-
-
In WormBook (The C. elegans Research Community, ed.).
-
Stiernagle, T. 2006. Maintenance of C. elegans. In WormBook (The C. elegans Research Community, ed.). http://www.wormbook.org/chapters/,www_strainmaintain/strainmaintain.html.
-
(2006)
Maintenance of C. elegans
-
-
Stiernagle, T.1
-
23
-
-
84884968656
-
Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPRCas9 system
-
Tzur, Y.B., Friedland, A.E., Nadarajan, S., Church, G.M., Calarco, J.A., and Colaiacovo, M.P. 2013. Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPRCas9 system. Genetics 195:1181-1185. doi: 10.1534/genetics.113.156075.
-
(2013)
Genetics
, vol.195
, pp. 1181-1185
-
-
Tzur, Y.B.1
Friedland, A.E.2
Nadarajan, S.3
Church, G.M.4
Calarco, J.A.5
Colaiacovo, M.P.6
-
24
-
-
58749085019
-
Agarose gel electrophoresis
-
Voytas, D. 2000. Agarose gel electrophoresis. Curr. Protoc. Mol. Biol. 51:2.5A.1-2.5A.9. Ward, J.D. 2015. Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair. Genetics 199:363-377. doi: 10.1534/genetics.114.172361.
-
(2000)
Curr. Protoc. Mol. Biol.
, vol.51
, pp. 2.5A.1-2.5A.9.
-
-
Voytas, D.1
-
25
-
-
84926451490
-
Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair
-
Ward, J.D. 2015. Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair. Genetics 199:363-377. doi: 10.1534/genetics.114.172361.
-
(2015)
Genetics
, vol.199
, pp. 363-377
-
-
Ward, J.D.1
-
26
-
-
0026749345
-
A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites
-
Williams, B.D., Schrank, B., Huynh, C., Shownkeen, R., and Waterston, R.H. 1992. A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131:609-624.
-
(1992)
Genetics
, vol.131
, pp. 609-624
-
-
Williams, B.D.1
Schrank, B.2
Huynh, C.3
Shownkeen, R.4
Waterston, R.H.5
|