Oligolysine-based coating protects DNA nanostructures from low-salt denaturation and nuclease degradation

Nature Communications

Volumn 8, Issue , 2017, Pages

  Ponnuswamy, Nandhini ^a,b,c   Bastings, Maartje M C ^a,b,c   Nathwani, Bhavik ^a,b,c   Ryu, Ju Hee ^a,b,c,d   Chou, Leo Y T ^a,b,c   Vinther, Mathias ^e   Li, Weiwei Aileen ^c,f   Anastassacos, Frances M ^a,b,c   Mooney, David J ^c,f   Shih, William M ^a,b,c  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c HARVARD UNIVERSITY   (United States)

^d Korea Institute of Science and Technology   (South Korea)

^e AARHUS UNIVERSITY   (Denmark)

^f HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COPOLYMER; DEOXYRIBONUCLEASE I; LYSINE DERIVATIVE; NITROGEN; PHOSPHORUS; CATION; DEOXYRIBONUCLEASE; DNA; INORGANIC SALT; LYSINE; MACROGOL; MAGNESIUM; NANOMATERIAL; POLYMER;

CONCENTRATION (COMPOSITION); DEGRADATION; DIGESTION; DNA; FUNCTIONAL ROLE; GENETIC ANALYSIS; INDUCED RESPONSE; LABORATORY METHOD; POLYMER; RODENT; SALT; SERUM;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOAVAILABILITY; CONTROLLED STUDY; DENATURATION; DISTRIBUTION PARAMETERS; DNA STRUCTURE; ENZYME DEGRADATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOUSE; MOUSE MODEL; NONHUMAN; ANIMAL; BONE MARROW; C57BL MOUSE; CHEMISTRY; CYTOLOGY; DENDRITIC CELL; FEMALE; HUMAN; STATIC ELECTRICITY; SURFACE PROPERTY; TRANSMISSION ELECTRON MICROSCOPY; UMBILICAL VEIN ENDOTHELIAL CELL;

ANIMALS; BONE MARROW; CATIONS; DENDRITIC CELLS; DEOXYRIBONUCLEASES; DNA; FEMALE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; LYSINE; MAGNESIUM; MICE; MICE, INBRED C57BL; MICROSCOPY, ELECTRON, TRANSMISSION; NANOSTRUCTURES; NITROGEN; PHOSPHORUS; POLYETHYLENE GLYCOLS; POLYMERS; SALTS; STATIC ELECTRICITY; SURFACE PROPERTIES;

EID: 85019998190     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms15654     Document Type: Article

References (32)

1. Rothemund, P. W. K. Folding DNA to create nanoscale shapes and patterns. Nature 440, 297-302 (2006).
2. Douglas, S. M. et al. Self-Assembly of DNA into nanoscale three-dimensional shapes. Nature 459, 414-418 (2009).
3. Dietz, H., Douglas, S. M. & Shih, W. M. Folding DNA into twisted and curved nano-scale shapes. Science 325, 725-730 (2009).
4. Han, D. et al. DNA origami with complex curvatures in three-dimensional space. Science 332, 342-346 (2011).
5. Wei, B., Dai, M. & Yin, P. Complex shapes self-Assembled from single-stranded DNA tiles. Nature 485, 623-626 (2012).
6. Ke, Y., Ong, L. L., Shih, W. M. & Yin, P. Three-dimensional structures self-Assembled from DNA bricks. Science 338, 1177-1183 (2012).
7. Li, J. et al. Self-Assembled multivalent DNA nanostructures for noninvasive intracellular delivery of immunostimulatory CpG oligonucleotides. ACS Nano 5, 8783-8789 (2011).
8. Andersen, E. S. et al. Self-Assembly of a nanoscale DNA box with a controllable lid. Nature 459, 73-76 (2009).
9. Douglas, S. M., Bachelet, I. & Church, G. M. A logic-gated nanorobot for targeted transport of molecular payloads. Science 335, 831-834 (2012).
10. Liu, M. et al. A DNA tweezer-Actuated enzyme nanoreactor. Nat. Commun. 4, 2127 (2013).
11. Banerjee, A. et al. Controlled release of encapsulated cargo from a DNA icosahedron using a chemical trigger. Angew. Chem. Int. Ed. 52, 6854-6857 (2013).
12. Jahnen-Dechent, W. & Ketteler, M. Magnesium basics. Clin. Kidney J. 5, i3-i14 (2012).
13. Hahn, J., Wickham, S. F. J., Shih, W. M. & Perrault, S. D. Addressing the instability of DNA nanostructures in tissue culture. ACS Nano 8, 8765-8775 (2014).
14. Conway, J. W., McLaughlin, C. K., Castor, K. J. & Sleiman, H. DNA nanostructure serum stability: greater than the sum of its parts. Chem. Commun. 49, 1172-1174 (2013).
15. Mei, Q. et al. Stability of DNA origami nanoarrays in cell lysate. Nano Lett. 11, 1477-1482 (2011).
16. Surana, S., Bhatia, D. & Krishnan, Y. A. Method to study in vivo stability of DNA nanostructures. Methods 64, 94-100 (2013).
17. Cassinelli, V. et al. One-step formation of chain-Armour stabilized DNA nanostructures. Angew. Chem. Int. Ed. 54, 7795-7798 (2015).
18. Benson, E. et al. DNA rendering of polyhedral meshes at the nanoscale. Nature 523, 441-444 (2015).
19. Veneziano, R. et al. Designer nanoscale DNA assemblies programmed from the top down. Science 352, 1534 (2016).
20. Mikkilä, J. et al. Virus-encapulated DNA origami nanostructures for cellular delivery. Nano Lett. 14, 2196-2200 (2014).
21. Kiviaho, J. K. et al. Cationic polymers for DNA origami coating-examining their binding efficiency and tuning the enzymatic reaction rates. Nanoscale 8, 11674-11680 (2016).
22. Perrault, S. D. & Shih, W. M. Virus-inspired membrance encapsulation of DNA nanostructures to achieve in vivo stability. ACS Nano 8, 5132-5140 (2014).
23. Boussif, O. et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethyleneimine. Proc. Natl Acad. Sci. USA 92, 7297-7301 (1995).
24. van Vlerken, L. E., Vyas, T. K. & Amiji, M. M. Poly(ethylene glycol)-modified nanocarriers for tumor-Targeted and intracellular delivery. Pharm. Res. 24, 1405-1414 (2007).
25. Vanecko, S. & Laskowski, Sr M. Studies of the specificity of deoxyribonuclease I. III. Hydrolysis of chains carrying a monoesterified phosphate on carbon 50 . J. Biol. Chem. 236, 3312-3316 (1961).
26. Haubner, R. et al. Structural and functional aspects of RGD-containing cyclic pentapeptides as highly potent and selective integrin avb3 antagonists. J. Am. Chem. Soc. 118, 7461-7472 (1996).
27. Yoshinaga, T., Yasuda, K., Ogawa, Y. & Takakura, Y. Efficient uptake and rapid degradation of plasmid DNA by murine dendritic cells via a specific mechanism. Biochem. Biophys. Res. Commun. 299, 389-394 (2002)
28. Mekler, V. M. A photochemical technique to enhance sensitivity of detection of fluorescence resonance energy transfer. Photochem. Photobiol. 59, 615-620 (1994).
29. Rodriguez, P. L. et al. Minimal self peptides that inhibit phagocytic clearance and enhance delivery of nanoparticles. Science 339, 971-975 (2013)
30. Lin, C., Perrault, S. D., Kwak, M., Graf, F. & Shih, W. M. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Res. 41, e40 (2013).
31. Bhattacharya, P., Gopisetty, A., Ganesh, B. B., Sheng, J. R. & Prabhakar, B. S. GM-CSF-induced, bone-marrow-derived dendritic cells can expand natural Tregs and induce adaptive Tregs by different mechanisms. J. Leukoc. Biol. 89, 235-249 (2011).
32. Douglas, S. M. et al. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001-5006 (2009).