Use of genetically modified bacteria for drug delivery in humans: Revisiting the safety aspect

Scientific Reports

Volumn 7, Issue 1, 2017, Pages

  Wegmann, Udo ^a   Carvalho, Ana Lucia ^a,b   Stocks, Martin ^c   Carding, Simon R ^a,b  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^c Plant Biotechnology Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; BACTEROIDES; DRUG DELIVERY SYSTEM; GASTROINTESTINAL TRACT; GENETIC ENGINEERING; GENETICS; HORIZONTAL GENE TRANSFER; HUMAN; MICROBIOLOGY; ORGANISMAL INTERACTION; PROCEDURES; TRANSGENE;

ANIMALS; BACTEROIDES; DRUG DELIVERY SYSTEMS; GASTROINTESTINAL TRACT; GENE TRANSFER, HORIZONTAL; GENETIC ENGINEERING; HUMANS; MICROBIAL INTERACTIONS; TRANSGENES;

EID: 85019606633     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-017-02591-6     Document Type: Article

References (32)

1. Cano-Garrido, O., Seras-Franzoso, J. & Garcia-Fruitos, E. Lactic acid bacteria: reviewing the potential of a promising delivery live vector for biomedical purposes. Microb Cell Fact 14, 137-149, doi:10.1186/s12934-015-0313-6 (2015).
2. Daniel, C., Roussel, Y., Kleerebezem, M. & Pot, B. Recombinant lactic acid bacteria as mucosal biotherapeutic agents. Trends Biotechnol 29, 499-508, doi:10.1016/j.tibtech.2011.05.002 (2011).
3. Robert, S. & Steidler, L. Recombinant Lactococcus lactis can make the difference in antigen-specific immune tolerance induction, the type 1 diabetes case. Microb Cell Fact 13, Suppl 1:S11, doi:10.1186/1475-2859-13-S1-S11 (2014).
4. Mimee, M., Tucker, A. C., Voigt, Christopher, A. & Lu, T. K. Programming a human commensal bacterium, Bacteroides thetaiotaomicron, to sense and respond to stimuli in the murine gut microbiota. Cell Syst 1, 62-71, doi.org/10.1016/j.cels.2015.06.001 (2015).
5. Kong, W., Kapuganti, V. S. & Lu, T. A gene network engineering platform for lactic acid bacteria. Nucleic Acids Res 44, e37, doi. org/10.1093/nar/gkv1093 (2015).
6. de Vos, W. M. Gene expression systems for lactic acid bacteria. Curr Opin Microbiol 2, 289-295, doi:10.1016/S1369-5274(99)80050-2 (1999).
7. Braat, H. et al. A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease. Clin Gastroenterol Hepatol 4, 754-759, doi:10.1016/j.cgh.2006.03.028 (2006).
8. Farrar, M. D. et al. Engineering of the gut commensal bacterium Bacteroides ovatus to produce and secrete biologically active murine interleukin-2 in response to xylan. J Appl Microbiol 98, 1191-1197, doi:10.1111/jam.2005.98.issue-5 (2005).
9. Hamady, Z. Z. R. et al. Identification and use of the putative Bacteroides ovatus xylanase promoter for the inducible production of recombinant human proteins. Microbiology 154, 3165-3174, doi:10.1099/mic.0.2008/019109-0 (2008).
10. Hamady, Z. Z. R. et al. Xylan-regulated delivery of human keratinocyte growth factor-2 to the inflamed colon by the human anaerobic commensal bacterium Bacteroides ovatus. Gut 59, 461-469, doi:10.1136/gut.2008.176131 (2010).
11. Steidler, L. et al. Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10. Nat Biotechnol 21, 785-789, doi:10.1038/nbt840 (2003).
12. Barner, H. D. & Cohen, S. S. The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. J Bacteriol. 68, 80-88 (1954).
13. Ahmad, S. I., Kirk, S. H. & Eisenstark, A. Thymine metabolism and thymineless death in prokaryotes and eukaryotes. Annu Rev Microbiol 52, 591-625, doi:10.1146/annurev.micro.52.1.591 (1998).
14. Tang, Y. P. Identification and Characterization of Genes in Bacteroides fragilis Involved in Aerotolerance. Department of Molecular Biology & Microbiology (Tufts University, 2000).
15. Syvanen, M. & Kado, C. I. Horizontal Gene Transfer, Edn. 2nd (Academic Press, San Diego, 2002).
16. Whittle, G., Hamburger, N., Shoemaker, N. B. & Salyers, A. A. A Bacteroides conjugative transposon, CTnERL, can transfer a portion of itself by conjugation without excising from the chromosome. J. Bacteriol 188, 1169-1174, doi:10.1128/JB.188.3.1169-1174.2006 (2006).
17. Ishibashi, M., Sugino, Y. & Hirota, Y. Chromosomal location of thymine and arginine genes in Escherichia coli and F' incorporating them. J. Bacteriol 87, 554-561 (1964).
18. Gasson, M. J. et al. Characterization and exploitation of conjugation in Lactococcus lactis. Int Dairy J 5, 757-762, doi:10.1016/0958-6946(95)00030-5 (1995).
19. Godon, J. J., Pillidge, C. J., Jury, K. & Gasson, M. J. Characterization of an original conjugative element: The sex factor of Lactococcus lactis 712. Lait 76, 41-49, doi:10.1051/lait:19961-25 (1996).
20. Levin, B. R. & Bergstrom, C. T. Bacteria are different: observations, interpretations, speculations, and opinions about the mechanisms of adaptive evolution in prokaryotes. Proc Natl Acad Sci USA 97, 6981-6985, doi:10.1073/pnas.97.13.6981 (2000).
21. Hansen, M. T. Multiplicity of genome equivalents in the radiation-resistant bacterium Micrococcus radiodurans. J Bacteriol 134, 71-75 (1978).
22. Tobiason, D. M. & Seifert, H. S. The obligate human pathogen, Neisseria gonorrhoeae, is polyploid. PLoS Biol 4, 1069-1078, doi:10.1371/journal.pbio.0040185 (2006).
23. Lobnerolesen, A., Atlung, T. & Rasmussen, K. V. Stability and replication control of Escherichia coli minichromosomes. J. Bacteriol 169, 2835-2842, doi:10.1128/jb.169.6.2835-2842.1987 (1987).
24. Bankevich, A. et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19, 455-477, doi:10.1089/cmb.2012.0021 (2012).
25. Wegmann, U., Goesmann, A. & Carding, S. R. Complete genome sequence of Bacteroides ovatus V975. Genome Announc 4, e01335-16, doi:10.1128/genomeA.01335-16 (2016).
26. Staden, R., Beal, K. F. & Bonfield, J. K. The Staden package, 1998. Methods Mol Biol 132, 115-130, doi:10.1385/1-59259-192-2:115 (2000).
27. Gurevich, A., Saveliev, V., Vyahhi, N. & Tesler, G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 29, 1072-1075, doi:10.1093/bioinformatics/btt086 (2013).
28. Michelsen, O., Hansen, F. G., Albrechtsen, B. & Jensen, P. R. The MG1363 and IL1403 laboratory strains of Lactococcus lactis and several dairy strains are diploid. J. Bacteriol 192, 1058-1065, doi:10.1128/JB.00900-09 (2010).
29. Figurski, D. H. & Helinski, D. R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76, 1648-1652, doi:10.1073/pnas.76.4.1648 (1979).
30. Shoemaker, N. B., Getty, C., Gardner, J. F. & Salyers, A. A. Tn4351 transposes in Bacteroides spp and mediates the integration of plasmid R751 into the Bacteroides chromosome. J. Bacteriol 165, 929-936, doi:10.1128/jb.165.3.929-936.1986 (1986).
31. Smith, C. J., Rollins, L. A. & Parker, A. C. Nucleotide sequence determination and genetic analysis of the Bacteroides plasmid, pBI143. Plasmid 34, 211-222, doi:10.1006/plas.1995.0007 (1995).
32. Wegmann, U., Horn, N. & Carding, S. R. Defining the bacteroides ribosomal binding site. Appl Environ Microbiol 79, 1980-1989, doi:10.1128/AEM.03086-12 (2013).