Molecular detection of tick-borne pathogens in wild red foxes (Vulpes vulpes) from Central Italy

Acta Tropica

Volumn 172, Issue , 2017, Pages 197-200

  Ebani, Valentina Virginia ^a   Rocchigiani, Guido ^a   Nardoni, Simona ^a   Bertelloni, Fabrizio ^a   Vasta, Violetta ^a   Papini, Roberto Amerigo ^a   Verin, Ranieri ^b   Poli, Alessandro ^a   Mancianti, Francesca ^a  

^a UNIVERSITY OF PISA   (Italy)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Red fox (Vulpes vulpes); Ticks; Vector borne pathogens; Zoonoses

Indexed keywords

DISEASE VECTOR; DNA; MAMMAL; MOLECULAR ANALYSIS; PARASITE; PATHOGEN;

ANAPLASMA PHAGOCYTOPHILUM; ARTICLE; BABESIA; BACTERIUM IDENTIFICATION; COXIELLA BURNETII; EHRLICHIA CANIS; FRANCISELLA TULARENSIS; HEPATOZOON CANIS; ITALY; MICROORGANISM DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; THEILERIA; THEILERIA ANNAE; TICK BORNE DISEASE; VULPES VULPES; ANIMAL; FOX; MICROBIOLOGY; PARASITOLOGY; TICK-BORNE DISEASES; VETERINARY;

ITALY; PISA [TUSCANY]; TUSCANY;

ANAPLASMA PHAGOCYTOPHILUM; ANIMALIA; BABESIA SP.; CANIDAE; CANIS FAMILIARIS; COXIELLA BURNETII; EHRLICHIA CANIS; FRANCISELLA TULARENSIS; HEPATOZOON CANIS; IXODIDA; THEILERIA ANNAE; THEILERIA SP.; VULPES; VULPES VULPES;

ANIMALS; FOXES; ITALY; POLYMERASE CHAIN REACTION; TICK-BORNE DISEASES;

EID: 85019245305     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2017.05.014     Document Type: Article

References (36)

1. Angelakis, E., Raoult, D., Q fever. Vet. Microbiol. 140 (2010), 297–309.
2. Baneth, G., Perspectives on canine and feline hepatozoonosis. Vet. Parasitol. 181 (2011), 3–11.
3. Bartley, P.M., Hamilton, C., Wilson, C., Innes, E.A., Katzer, F., Detection of Babesia annae DNA in lung exudate samples from red foxes (Vulpes vulpes) in Great Britain. Parasit. Vectors, 9, 2016, 84, 10.1186/s13071-016-1364-1.
4. Beck, R., Vojta, L., Mrljak, V., Marinculić, A., Beck, A., Živičnjak, T., Cacciò, S.M., Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. Int. J. Parasitol. 39 (2009), 843–848.
5. Berri, M., Rekiki, A., Boumedine, A., Rodolakis, A., Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum, and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR. BMC Microbiol., 9, 2009, 130, 10.1186/1471-2180-9-130.
6. Cardoso, L., Cortes, H.C., Eyal, O., Reis, A., Lopes, A.P., Vila-Viçosa, M.J., Rodrigues, P.A., Baneth, G., Molecular and histopathological detection of Hepatozoon canis in red foxes (Vulpes vulpes) from Portugal. Parasit. Vectors, 7, 2014, 113, 10.1186/1756-3305-7-113.
7. Cardoso, L., Cortes, H.C., Reis, A., Rodrigues, P., Simões, M., Lopes, A.P., Vila-Viçosa, M.J., Talmi-Frank, D., Eyal, O., Solano-Gallego, L., Baneth, G., Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal. Vet. Parasitol. 196 (2013), 90–95.
8. Cardoso, L., Gilad, M., Cortes, H.C., Nachum-Biala, Y., Lopes, A.P., Vila-Viçosa, M.J., Simoes, M., Rodrigues, P.A., Baneth, G., First report of Anaplasma platys infection in red foxes (Vulpes vulpes) and molecular detection of Ehrlichia canis and Leishmania infantum in foxes from Portugal. Parasit. Vectors, 8, 2015, 144, 10.1186/s13071-015-0756-y.
9. Dawson, J.E., Stallknecht, D.E., Howerth, E.W., Warner, C., Biggie, K., Davidson, W.R., Lockhart, J.M., Nettles, V.F., Olson, J.G., Childs, J.E., Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. J. Clin. Microbiol. 32 (1994), 2725–2728.
10. Dumitrache, M.O., Matei, I.A., Ionică, A.M., Kalmár, Z., D'Amico, G., Sikó-Barabási, S., Ionescu, D.T., Gherman, C.M., Mihalca, A.D., Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato genospecies in red foxes (Vulpes vulpes) from Romania. Parasit. Vectors, 8, 2015, 514, 10.1186/s13071-015-1130-9.
11. Duscher, G.G., Fuehrer, H.P., Kübber-Heiss, A., Fox on the run – molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria. Parasit. Vectors, 7, 2014, 521, 10.1186/s13071-014-0521-7.
12. Ebani, V.V., Nardoni, S., Fognani, G., Mugnaini, L., Bertelloni, F., Rocchigiani, G., Papini, R.A., Stefani, F., Mancianti, F., Molecular detection of vector-borne bacteria and protozoa in healthy hunting dogs from Central Italy. Asian Pac. J. Trop. Biomed. 5 (2015), 108–112.
13. Ebani, V.V., Rocchigiani, G., Bertelloni, F., Nardoni, S., Leoni, A., Nicoloso, S., Mancianti, F., Molecular survey on the presence of zoonotic arthropod-borne pathogens in wild red deer (Cervus elaphus). Comp. Immunol. Microb. 47 (2016), 77–80.
14. Ebani, V.V., Verin, R., Fratini, F., Poli, A., Cerri, D., Molecular survey of Anaplasma phagocytophilum and Ehrlichia canis in Red Foxes (Vulpes vulpes) from Central Italy. J. Wildl. Dis. 47 (2011), 699–703.
15. Farkas, R., Takacs, N., Hornyak, A., Nachum-Biala, Y., Hornok, S., Baneth, G., First report on Babesia cf. microti infection of red foxes (Vulpes vulpes) from Hungary. Parasit. Vectors, 8, 2015, 55, 10.1186/s13071-015-0660-5.
16. Gabrielli, S., Kumlien, S., Calderini, P., Brozzi, A., Iori, A., Cancrini, G., The first report of Hepatozoon canis identified in Vulpes vulpes and ticks from Italy. Vector Borne Zoonotic Dis. 10 (2010), 855–859.
17. Hartwig, V., von Loewenich, F.D., Schultze, C., Straubinger, R.K., DAugschies, A., Dyachenko, V., Detection of Anaplasma phagocytophilum in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procynoides) from Branderburg, Germany. Ticks Tick Borne Dis. 5 (2014), 277–280.
18. Harvey, J.W., Simpson, C.F., Gaskin, J.M., Sameek, J.H., Ehrlichiosis in wolves, dogs, and wolf-dog crosses. J. Am. Vet. Med. Assoc. 175 (1979), 901–905.
19. Hestvik, G., Warns-Petit, E., Smith, L.A., Fox, N.J., Uhlhorn, H., Artois, M., Hannant, D., Hutchings, M.R., Mattsson, R., Yon, L., Gavier-Widen, D., The status of tularemia in Europe in a one-health context: a review. Epidemiol. Infect. 143 (2015), 2137–2160.
20. Hodžić, A., Alić, A., Fuehrer, H.P., Harl, J., Wille-Piazzai, W., Duscher, G.G., A molecular survey of vector-borne pathogens in red foxes (Vulpes vulpes) from Bosnia and Herzegovina. Parasit. Vectors, 8, 2015, 88, 10.1186/s13071-015-0692-x.
21. Hulinská, D., Langrová, K., Pejcoch, M., Pavlásek, I., Detection of Anaplasma phagocytophilum in animals by real-time polymerase chain reaction. APMIS 112 (2004), 239–247.
22. Karbowiak, G., Vichova, B., Majlathova, V., Hapunik, J., Petko, B., Anaplasma phagocytophilum infection of red foxes (Vulpes vulpes). Ann. Agric. Environ. Med. 16 (2009), 299–300.
23. Kuehn, A., Schulze, C., Kutzer, P., Probst, C., Hlinak, A., Ochs, A., Grunow, R., Tularaemia seroprevalence of captured and wild animals in Germany: the fox (Vulpes vulpes) as a biological indicator. Epidemiol. Infect. 141 (2013), 833–840.
24. Liesner, J.M., Krücken, J., Schaper, R., Pachnicke, S., Kohn, B., Müller, E., Schulze, C., von Samson-Himmelstjerna, G., Vector-borne pathogens in dogs and red foxes from the federal state of Brandenburg, Germany. Vet. Parasitol. 224 (2016), 44–51.
25. Massung, R.F., Slater, K., Owens, J.H., Nicholson, W.L., Mather, T.N., Solberg, V.B., Olson, J.G., Nested PCR assay for detection of granulocytic Ehrlichiae. J. Clin. Microbiol. 36 (1998), 1090–1095.
26. Meredith, A.L., Cleaveland, S.C., Denwood, M.J., Brown, J.K., Shaw, D.J., Coxiella burnetii (Q-fever) seroprevalence in prey and predators in the United Kingdom: evaluation of infection in wild rodents, foxes and domestic cats using a modified ELISA. Transbound. Emerg. Dis. 62 (2015), 639–649.
27. Millán, J., Proboste, T., Fernández de Mera, I.G., Chirife, A.D., de la Fuente, J., Altet, L., Molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface. Ticks Tick Borne Dis. 7 (2016), 284–290.
28. Milutinović, M., Masuzawa, T., Tomanović, S., Radulović, Z., Fukui, T., Okamoto, Y., Borrelia burgdorferi sensu lato, Anaplasma phagcoytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia. Exp. Appl. Acarol. 45 (2008), 171–183.
29. Mitková, B., Hrazdilová, K., Steinbauer, V., D'Amico, G., Mihalca, A.D., Modrý, D., Autochthonous Hepatozoon infection in hunting dogs and foxes from the Czech Republic. Parasitol. Res. 115 (2016), 4167–4171.
30. Najm, N.A., Meyer-Kayser, E., Hoffmann, L., Herb, I., Fensterer, V., Pfister, K., Silaghi, C., A molecular survey of Babesia spp. and Theileria spp. in red foxes (Vulpes vulpes) and their ticks from Thuringia, Germany. Ticks Tick Borne Dis. 5 (2014), 386–391.
31. Pascucci, I., Di Domenico, M., Dall'Acqua, F., Sozio, G., Cammà, C., Detection of Lyme disease and Q fever agents in wild rodents in central Italy. Vector Borne Zoonotic Dis. 15 (2015), 404–411.
32. Psaroulaki, A., Chochlakis, D., Ioannou, I., Angelakis, E., Tselentis, Y., Presence of Coxiella burnetii in fleas in Cyprus. Vector Borne Zoonotic Dis. 14 (2014), 685–687.
33. Psaroulaki, A., Chochlakis, D., Angelakis, E., Ioannou, I., Tselentis, Y., Coxiella burnetii in wildlife and ticks in an endemic area. Trans. R. Soc. Trop. Med. Hyg. 108 (2014), 625–631.
34. Santoro, M., Veneziano, V., D'Alessio, N., Di Prisco, F., Lucibelli, M.G., Borriello, G., Cerrone, A., Dantas-Torres, F., Latrofa, M.S., Otranto, D., Galiero, G., Molecular survey of Ehrlichia canis and Coxiella burnetii infections in wild mammals of southern Italy. Parasitol. Res. 115 (2016), 4427–4431.
35. Zanet, S., Trisciuoglio, A., Bottero, E., de Mera, I.G., Gortazar, C., Carpignano, M.G., Ferroglio, E., Piroplasmosis in wildlife: Babesia and Theileria affecting free-ranging ungulates and carnivores in the Italian Alps. Parasit. Vectors, 7, 2014, 70, 10.1186/1756-3305-7-70.
36. Wen, B., Rikihisa, Y., Mott, J.M., Greene, R., Kim, H.Y., Zhi, N., Couto, G.C., Unver, A., Bartsch, R., Comparison of nested PCR with immunofluorescent antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. J. Clin. Microbiol. 35 (1997), 1852–1855.