Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering

International Journal of Biological Macromolecules

Volumn 102, Issue , 2017, Pages 1174-1185

  Gomes, Susana ^a   Rodrigues, Gabriela ^b   Martins, Gabriel ^b,c   Henriques, Célia ^a   Silva, Jorge Carvalho ^a  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

^c INSTITUTO GULBENKIAN DE CIÊNCIA   (Portugal)

Author keywords

Blends; Chitosan; Electrospinning; Gelatin; Polycaprolactone; Skin substitutes

Indexed keywords

ACETIC ACID; CHITOSAN; GELATIN; GLUTARALDEHYDE; MOLECULAR SCAFFOLD; POLYCAPROLACTONE; WATER; BIOMATERIAL; NANOFIBER; POLYESTER;

ARTICLE; CELL ADHESION; CELL GROWTH; CELL PROLIFERATION; CELL STRUCTURE; CHEMICAL BOND; CONDUCTANCE; CONTACT ANGLE; CONTROLLED STUDY; CROSS LINKING; ELECTROSPINNING; FIBROBLAST; HUMAN; HUMAN CELL; HYDROPHILICITY; POROSITY; SKIN; SURFACE TENSION; TISSUE ENGINEERING; VISCOSITY; YOUNG MODULUS; CELL LINE; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; EXTRACELLULAR MATRIX; MECHANICS; METABOLISM; TISSUE SCAFFOLD; WETTABILITY;

BIOCOMPATIBLE MATERIALS; CELL ADHESION; CELL LINE; CELL PROLIFERATION; CHITOSAN; EXTRACELLULAR MATRIX; GELATIN; HUMANS; MECHANICAL PHENOMENA; NANOFIBERS; POLYESTERS; POROSITY; SKIN; TISSUE ENGINEERING; TISSUE SCAFFOLDS; WETTABILITY;

EID: 85019132043     PISSN: 01418130     EISSN: 18790003     Source Type: Journal    
DOI: 10.1016/j.ijbiomac.2017.05.004     Document Type: Article

References (54)

1. Goel, A., Shrivastava, P., Post-burn scars and scar contractures. Indian J. Plast. Surg. 43 (2010), S63–S71, 10.4103/0970-0358.70724.
2. Han, T., Teissler, H., Han, R.J., Gaines, J.D., Nguyen, T.Q., Case report managing difficult airway in patients with post-burn mentosternal and circumoral scar contractures. Int. J. Burns Trauma 2 (2012), 80–85.
3. Fufa, D.T., Chuang, S.S., Yang, J.Y., Prevention and surgical management of postburn contractures of the hand. Curr. Rev. Musculoskelet. Med. 7 (2014), 53–59, 10.1007/s12178-013-9192-9.
4. Martin, P., Wound healing-aiming for perfect skin regeneration. Science 276 (1997), 75–81, 10.1126/science.276.5309.75.
5. Nandan, D., Cates, G.A., Ball, E.H., Sanwal, B.D., A collagen-binding protein involved in the differentiation of myoblasts recognizes the Arg-Gly-Asp sequence. Exp. Cell Res. 179 (1988), 289–297, 10.1016/0014-4827(88)90368-0.
6. Huang, Y., Onyeri, S., Siewe, M., Moshfeghian, A., Madihally, S.V., In vitro characterization of chitosan-gelatin scaffolds for tissue engineering. Biomaterials 26 (2005), 7616–7627, 10.1016/j.biomaterials.2005.05.036.
7. Broderick, E.P., O'Halloran, D.M., Rochev, Y.a., Griffin, M., Collighan, R.J., Pandit, A.S., Enzymatic stabilization of gelatin-based scaffolds. J. Biomed. Mater. Res.—Part B: Appl. Biomater. 72 (2005), 37–42, 10.1002/jbm.b.30119.
8. Gomes, S.R., Rodrigues, G., Martins, G.G., Henriques, C.M.R., Silva, J.C., In vitro evaluation of crosslinked electrospun fish gelatin scaffolds. Mater. Sci. Eng. C 33 (2013), 1219–1227, 10.1016/j.msec.2012.12.014.
9. Ko, J.H., Yin, H., An, J., Chung, D.J., Kim, J.H., Lee, S.B., Pyun, D.G., Characterization of cross-linked gelatin nanofibers through electrospinning. Macromol. Res. 18 (2010), 137–143, 10.1007/s13233-009-0103-2.
10. Bae, H.J., Darby, D.O., Kimmel, R.M., Park, H.J., Whiteside, W.S., Effects of transglutaminase-induced cross-linking on properties of fish gelatin-nanoclay composite film. Food Chem. 114 (2009), 180–189, 10.1016/j.foodchem.2008.09.057.
11. Sisson, K., Zhang, C., Farach-Carson, M.C., Chase, D.B., Rabolt, J.F., Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability. Biomacromolecules 10 (2009), 1675–1680, 10.1021/bm900036s.
12. Gomes, S.R., Rodrigues, G., Martins, G.G., Roberto, M.a., Mafra, M., Henriques, C.M.R., Silva, J.C., In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: a comparative study. Mater. Sci. Eng. C 46 (2015), 348–358, 10.1016/j.msec.2014.10.051.
13. Sionkowska, A., Current research on the blends of natural and synthetic polymers as new biomaterials: review. Prog. Polym. Sci. 36 (2011), 1254–1276, 10.1016/j.progpolymsci.2011.05.003.
14. Zhang, Y., Ouyang, H., Chwee, T.L., Ramakrishna, S., Huang, Z.M., Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J. Biomed. Mater. Res.—Part B: Appl. Biomater. 72 (2005), 156–165, 10.1002/jbm.b.30128.
15. Chong, E.J., Phan, T.T., Lim, I.J., Zhang, Y.Z., Bay, B.H., Ramakrishna, S., Lim, C.T., Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater. 3 (2007), 321–330, 10.1016/j.actbio.2007.01.002.
16. Ghasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.-H., Ramakrishna, S., Electrospun poly(ε-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29 (2008), 4532–4539, 10.1016/j.biomaterials.2008.08.007.
17. Duan, H., Feng, B., Guo, X., Wang, J., Zhao, L., Zhou, G., Liu, W., Cao, Y., Zhang, W.J., Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes. Int. J. Nanomed. 8 (2013), 2077–2084, 10.2147/IJN.S42384.
18. Shi, R., Xue, J., Wang, H., Wang, R., Gong, M., Chen, D., Zhang, L., Tian, W., Fabrication and evaluation of a homogeneous electrospun PCL-gelatin hybrid membrane as an anti-adhesion barrier for craniectomy. J. Mater. Chem. B 3 (2015), 4063–4073, 10.1039/C5TB00261C.
19. Xue, J., He, M., Liang, Y., Crawford, A., Coates, P., Chen, D., Shi, R., Zhang, L., Fabrication and evaluation of electrospun PCL-gelatin micro-/nanofiber membranes for anti-infective GTR implants. J. Mater. Chem. B 2 (2014), 6867–6877, 10.1039/C4TB00737A.
20. Mao, J., Zhao, L., De Yao, K., Shang, Q., Yang, G., Cao, Y., Study of novel chitosan-gelatin artificial skin in vitro. J. Biomed. Mater. Res. A 64 (2003), 301–308, 10.1002/jbm.a.10223.
21. Thein-Han, W.W., Saikhun, J., Pholpramoo, C., Misra, R.D.K., Kitiyanant, Y., Chitosan-gelatin scaffolds for tissue engineering: physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP-buffalo embryonic stem cells. Acta Biomater. 5 (2009), 3453–3466, 10.1016/j.actbio.2009.05.012.
22. Chiono, V., Pulieri, E., Vozzi, G., Ciardelli, G., Ahluwalia, A., Giusti, P., Genipin-crosslinked chitosan/gelatin blends for biomedical applications. J. Mater. Sci. Mater. Med. 19 (2007), 889–898, 10.1007/s10856-007-3212-5.
23. Qian, Y.-F., Zhang, K.-H., Chen, F., Ke, Q.-F., Mo, X.-M., Cross-linking of gelatin and chitosan complex nanofibers for tissue-engineering scaffolds. J. Biomater. Sci. Polym. Ed. 22 (2011), 1099–1113, 10.1163/092050610X499447.
24. Franco, R.A., Nguyen, T.H., Lee, B.T., Preparation and characterization of electrospun PCL/PLGA membranes and chitosan/gelatin hydrogels for skin bioengineering applications. J. Mater. Sci. Mater. Med. 22 (2011), 2207–2218, 10.1007/s10856-011-4402-8.
25. Zargar, V., Asghari, M., Dashti, A., A review on chitin and chitosan polymers: structure, chemistry, solubility, derivatives, and applications. ChemBioEng Rev. 2 (2015), 204–226, 10.1002/cben.201400025.
26. Lee, K.Y., Jeong, L., Kang, Y.O., Lee, S.J., Park, W.H., Electrospinning of polysaccharides for regenerative medicine. Adv. Drug Deliv. Rev. 61 (2009), 1020–1032, 10.1016/j.addr.2009.07.006.
27. Mori, T., Murakami, M., Okumura, M., Kadosawa, T., Uede, T., Fujinaga, T., Mechanism of macrophage activation by chitin derivatives. J. Vet. Med. Sci. 67 (2005), 51–56, 10.1292/jvms.67.51.
28. Ueno, H., Yamada, H., Tanaka, I., Kaba, N., Matsuura, M., Okumura, M., Kadosawa, T., Fujinaga, T., Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials 20 (1999), 1407–1414, 10.1016/S0142-9612(99)00046-0.
29. Azad, A.K., Sermsintham, N., Chandrkrachang, S., Stevens, W.F., Chitosan membrane as a wound-healing dressing: characterization and clinical application. J. Biomed. Mater. Res. B: Appl. Biomater. 69 (2004), 216–222, 10.1002/jbm.b.30000.
30. Labet, M., Thielemans, W., Synthesis of polycaprolactone: a review. Chem. Soc. Rev. 38 (2009), 3484–3504, 10.1039/b820162p.
31. Li, W.-J., Cooper, J.a., Mauck, R.L., Tuan, R.S., Fabrication and characterization of six electrospun poly(α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomater. 2 (2006), 377–385, 10.1016/j.actbio.2006.02.005.
32. Qian, Y., Zhang, Z., Zheng, L., Song, R., Zhao, Y., Fabrication and characterization of electrospun polycaprolactone blended with chitosan-gelatin complex nanofibrous mats. J. Nanomater. 2014 (2014), 1–7, 10.1155/2014/964621.
33. Gautam, S., Chou, C.F., Dinda, A.K., Potdar, P.D., Mishra, N.C., Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications. J. Mater. Sci. 49 (2014), 1076–1089, 10.1007/s10853-013-7785-8.
34. Zhang, Y.Z., Su, B., Ramakrishna, S., Lim, C.T., Chitosan nanofibers from an easily electrospinnable UHMWPEO-doped chitosan solution system. Biomacromolecules 9 (2008), 136–141, 10.1021/bm701130e.
35. Schneider, C.A., Rasband, W.S., Eliceiri, K.W., NIH image to ImageJ: 25 years of image analysis. Nat. Methods 9 (2012), 671–675, 10.1038/nmeth.2089.
36. Zhu, X., Cui, W., Li, X., Jin, Y., Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules 9 (2008), 1795–1801, 10.1021/bm800476u.
37. Vega-Avila, E., Pugsley, M.K., An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells. Proc. West. Pharmacol. Soc. 54 (2011), 10–14.
38. Cooper, A., Bhattarai, N., Zhang, M., Fabrication and cellular compatibility of aligned chitosan-PCL fibers for nerve tissue regeneration. Carbohydr. Polym. 85 (2011), 149–156, 10.1016/j.carbpol.2011.02.008.
39. Kriegel, C., Kit, K.M., Mcclements, D.J., Weiss, J., Electrospinning of chitosan −poly (ethylene oxide) blend nanofibers in the presence of micellar surfactant solutions. Polymer (Guildf.) 50 (2009), 189–200, 10.1016/j.polymer.2008.09.041.
40. Chiono, V., Vozzi, G., D'Acunto, M., Brinzi, S., Domenici, C., Vozzi, F., Ahluwalia, A., Barbani, N., Giusti, P., Ciardelli, G., Characterisation of blends between poly(ε-caprolactone) and polysaccharides for tissue engineering applications. Mater. Sci. Eng. C 29 (2009), 2174–2187, 10.1016/j.msec.2009.04.020.
41. Silva, S.M.L., Braga, C.R.C., Fook, M.V.L., Raposo, C.M.O., Carvalho, L.H., Canedo, E.L., Application of infrared spectroscopy to analysis of chitosan/clay nanocomposites. Infrared Spectrosc.—Mater. Sci. Eng. Technol., 2012, 10.5772/35522 pp. 43–62.
42. Gautam, S., Dinda, A.K., Mishra, N.C., Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method. Mater. Sci. Eng. C 33 (2013), 1228–1235, 10.1016/j.msec.2012.12.015.
43. De Wael, K., Verstraete, A., Van Vlierberghe, S., Dejonghe, W., Dubruel, P., Adriaens, A., The electrochemistry of a gelatin modified gold electrode. Int. J. Electrochem. Sci. 6 (2011), 1810–1819.
44. Bellamy, L.J., The Infra-Red Spectra of Complex Molecules. 1975, Chapman and Hall, London.
45. Zhang, Y.Z., Venugopal, J., Huang, Z.M., Lim, C.T., Ramakrishna, S., Crosslinking of the electrospun gelatin nanofibers. Polymer (Guildf.) 47 (2006), 2911–2917, 10.1016/j.polymer.2006.02.046.
46. Namli, H., Turhan, O., Simultaneous observation of reagent consumption and product formation with the kinetics of benzaldehyde and aniline reaction in FTIR liquid cell. Vib. Spectrosc. 43 (2007), 274–283, 10.1016/j.vibspec.2006.02.010.
47. Schiffman, J.D., Schauer, C.L., Cross-linking chitosan nanofibers. Biomacromolecules 8 (2007), 594–601, 10.1021/bm060804s.
48. Feng, B., Tu, H., Yuan, H., Peng, H., Zhang, Y., Acetic-acid-mediated miscibility toward electrospinning homogeneous composite nanofibers of GT/PCL. Biomacromolecules 13 (2012), 3917–3925, 10.1021/bm3009389.
49. Prabhakaran, M.P., Venugopal, J.R., Ter Chyan, T., Hai, L.B., Chan, C.K., Lim, A.Y., Ramakrishna, S., Electrospun biocomposite nanofibrous scaffolds for neural tissue engineering. Tissue Eng. Part A 14 (2008), 1787–1797, 10.1089/ten.tea.2007.0393.
50. Prasad, T., Shabeena, E.A., Vinod, D., Kumary, T.V., Anil Kumar, P.R., Characterization and in vitro evaluation of electrospun chitosan/polycaprolactone blend fibrous mat for skin tissue engineering. J. Mater. Sci. Mater. Med., 26, 2015, 5352, 10.1007/s10856-014-5352-8.
51. Wang, T.J., Wang, I.J., Chen, S., Chen, Y.H., Young, T.H., The phenotypic response of bovine corneal endothelial cells on chitosan/polycaprolactone blends. Colloids Surf. B: Biointerfaces 90 (2012), 236–243, 10.1016/j.colsurfb.2011.10.043.
52. Bacakova, L., Filova, E., Parizek, M., Ruml, T., Svorcik, V., Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol. Adv. 29 (2011), 739–767, 10.1016/j.biotechadv.2011.06.004.
53. Luna, S.M., Silva, S.S., Gomes, M.E., Mano, J.F., Reis, R.L., Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification. J. Biomater. Appl. 26 (2011), 101–116, 10.1177/0885328210362924.
54. Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., Adhesive protein interactions with chitosan: consequences for valve endothelial cell growth on tissue-engineering materials. J. Biomed. Mater. Res. A 67 (2003), 538–547, 10.1002/jbm.a.10095.