MALDI-TOF MS and Filamentous Fungal Identification: A Success Story?

Current Fungal Infection Reports

Volumn 11, Issue 2, 2017, Pages 60-65

  Hendrickx, Marijke ^a  

^a SCIENTIFIC INSTITUTE OF PUBLIC HEALTH   (Belgium)

Author keywords

Antifungal resistance; Filamentous fungi; Identification; Invasive fungal disease; MALDI TOF MS

Indexed keywords

ACCURACY; ANALYTICAL ERROR; ANTIFUNGAL RESISTANCE; ANTIFUNGAL SUSCEPTIBILITY; ASPERGILLUS; CANDIDA; COST EFFECTIVENESS ANALYSIS; FILAMENTOUS FUNGUS; FUNGAL STRAIN; FUNGUS IDENTIFICATION; FUSARIUM; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MUCORALES; REVIEW;

EID: 85018845275     PISSN: 19363761     EISSN: 1936377X     Source Type: Journal    
DOI: 10.1007/s12281-017-0277-6     Document Type: Review

References (41)

1. Brown GD, Denning DW, Gow NAR, Levitz SM, Netea MG, White TC. Hidden killers: human fungal infections. Med Mycol. 2012;4(165):165rv13. doi:10.1126/scitranslmed.3004404.
2. Havlickova B, Czaika VA, Friedrich M. Epidemiological trends in skin mycoses worldwide. Mycoses. 2008;51(Suppl. 4):2–15.
3. Perfect JR. Fungal diagnosis: how do we do it and can we do better? Curr Med Res Opin. 2013;29(Suppl 4):3–11.
4. Clarck AE, Kaleta EJ, Arora A, Wolk DM. Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clin Micr Rev. 2013;26(3):547–603.
5. • Cassagne C, Normand AC, L’Ollivier C, Ranque S, Piarroux R. Performance of MALDI-TOF MS platforms for fungal identification. Mycoses. 2016;59(11):678–90. This review paper provides an excellent overview on the identification efficacy of yeasts, filamentous fungi and dermatophytes.
6. van Veen SQ, Claas EC, Kuijper EJ. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol. 2010;48(3):900–7.
7. Marklein G, Josten M, Klanke U, Müller E, Horré R, Maier T, Wenzel T, Kostrzewa M, Bierbaum G, Hoerauf A, Sahl HG. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J Clin Microbiol. 2009;47(9):2912–7.
8. Sanguinetti M, Posteraro B. Identification of molds by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2016;55(2):369–79.
9. Bader O, Weig M, Taverne-Ghadwal L, Lugert R, Gross U, Kuhns M. Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Microbiol Infect. 2011;17(9):1359–65.
10. Martiny D, Busson L, Wybo I, El Haj RA, Dediste A, Vandenberg O. Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012;50(4):1313–25.
11. Lacroix C, Gicquel A, Sendid B, Meyer J, Accoceberry I, François N, Morio F, Desoubeaux G, Chandenier J, Kauffmann-Lacroix C, Hennequin C, Guitard J, Nassif X, Bougnoux ME. Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems for the identification of Candida species. Clin Microbiol Infect. 2014;20(2):153–8.
12. • Lévesque S, Dufresne PJ, Soualhine H, Domingo MC, Bekal S, Lefebvre B, Tremblay C. A side by side comparison of Bruker Biotyper and VITEK MS: utility of MALDI-TOF MS technology for microorganism identification in a public health reference laboratory. PLoS One. 2015;10(12):e0144878. doi:10.1371/journal.pone.0144878. Excellent comparison between two available platforms for a wide variety of species.
13. Alanio A, Beretti JL, Dauphin B, Mellado E, Quesne G, Lacroix C, Amara A, Berche P, Nassif X, Bougnoux ME. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species. Clin Microbiol Infect. 2011;17(5):750–5.
14. De Carolis E, Posteraro B, Lass-Flörl C, Vella A, Florio AR, Torelli R, Girmenia C, Colozza C, Tortorano AM, Sanguinetti M, Fadda G. Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Microbiol Infect. 2012;18(5):475–84.
15. Coulibaly O, Marinach-Patrice C, Cassagne C, Piarroux R, Mazier D, Ranque S. Pseudallescheria/Scedosporium complex species identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Med Mycol. 2011;49(6):621–6.
16. Schulthess B, Ledermann R, Mouttet F, Zbinden A, Bloemberg GV, Böttger EC, Hombach M. Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory. J Clin Microbiol. 2014;52(8):2797–803.
17. Nenoff P, Erhard M, Simon JC, Muylowa GK, Herrmann J, Rataj W, Gräser Y. MALDI-TOF mass spectrometry—a rapid method for the identification of dermatophyte species. Med Mycol. 2013;51(1):17–24.
18. Lau AF, Drake SK, Calhoun LB, Henderson CM, Zelazny AM. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2013;51(3):828–34.
19. Cassagne C, Ranque S, Normand AC, Fourquet P, Thiebault S, Planard C, Hendrickx M, Piarroux R. Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. PLoS One. 2011;6(12):e28425.
20. Ranque S, Normand AC, Cassagne C, Murat JB, Bourgeois N, Dalle F, Gari-Toussaint M, Fourquet P, Hendrickx M, Piarroux R. MALDI-TOF mass spectrometry identification of filamentous fungi in the clinical laboratory. Mycoses. 2014;57(3):135–40.
21. Normand AC, Cassagne C, Ranque S, L’ollivier C, Fourquet P, Roesems S, Hendrickx M, Piarroux R. Assessment of various parameters to improve MALDI-TOF MS reference spectra libraries constructed for the routine identification of filamentous fungi. BMC Microbiol. 2013;13:76.
22. Becker PT, de Bel A, Martiny D, Ranque S, Piarroux R, Cassagne C, Detandt M, Hendrickx M. Identification of filamentous fungi isolates by MALDI-TOF mass spectrometry: clinical evaluation of an extended reference spectra library. Med Mycol. 2014;52(8):826–34.
23. Triest D, Stubbe D, De Cremer K, Piérard D, Normand AC, Piarroux R, Detandt M, Hendrickx M. Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of molds of the Fusarium genus. J Clin Microbiol. 2015;53(2):465–76.
24. Iriart X, Lavergne RA, Fillaux J, Valentin A, Magnaval JF, Berry A, Cassaing S. Routine identification of medical fungi by the new Vitek MS matrix-assisted laser desorption ionization-time of flight system with a new time-effective strategy. J Clin Microbiol. 2012;50(6):2107–10.
25. Oliveira MM, Santos C, Sampaio P, Romeo O, Almeida-Paes R, Pais C, Lima N, Zancopé-Oliveira RM. Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex. Res Microbiol. 2015;166(2):102–10.
26. Bille E, Dauphin B, Leto J, Bougnoux ME, Beretti JL, Lotz A, Suarez S, Meyer J, Join-Lambert O, Descamps P, Grall N, Mory F, Dubreuil L, Berche P, Nassif X, Ferroni A. MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures. Clin Microbiol Infect. 2012;18(11):1117–25.
27. Dolatabadi S, Kolecka A, Versteeg M, de Hoog SG, Boekhout T. Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). J Med Microbiol. 2015;64(7):694–701.
28. Packeu A, De Bel A, l’Ollivier C, Ranque S, Detandt M, Hendrickx M. Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory. J Clin Microbiol. 2014;52(9):3440–3.
29. L’Ollivier C, Cassagne C, Normand AC, Bouchara JP, Contet-Audonneau N, Hendrickx M, Fourquet P, Coulibaly O, Piarroux R, Ranque S. A MALDI-TOF MS procedure for clinical dermatophyte species identification in the routine laboratory. Med Mycol. 2013;51(7):713–20.
30. Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49(12):4067–71.
31. Mery A, Sendid B, François N, Cornu M, Poissy J, Guerardel Y, Poulain D. Application of mass spectrometry technology to early diagnosis of invasive fungal infections. J Clin Microbiol. 2016;54(11):2786–97.
32. •• de Almeida JN Jr, Sztajnbok J, da Silva AR Jr, Vieira VA, Galastri AL, Bissoli L, Litvinov N, Del Negro GM, Motta AL, Rossi F, Benard G. Rapid identification of moulds and arthroconidial yeasts from positive blood cultures by MALDI-TOF mass spectrometry. Med Mycol. 2016;54(8):885–9. Paper describes promising new application of MALDI-TOF MS directly on clinical specimens.
33. •• Triest D, Hendrickx M, Piérard D, Piarroux R, Fraselle S, De Cremer K. Proof-of-concept study of a new LC-ESI-MS/MS-basd assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides. Mycol Progress. 2017; doi:10.1007/s11557-017-1273-5. Paper describes promising new MS/MS approach for the detection of species specific peptides.
34. Marinach C, Alanio A, Palous M, Kwasek S, Fekkar A, Brossas JY, Brun S, Snounou G, Hennequin C, Sanglard D, Datry A, Golmard JL, Mazier D. MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fluconazole. Proteomics. 2009;9(20):4627–31.
35. Vella A, De Carolis E, Vaccaro L, Posteraro P, Perlin DS, Kostrzewa M, Posteraro B, Sanguinetti M. Rapid antifungal susceptibility testing by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. J Clin Microbiol. 2013;51(9):2964–9.
36. De Carolis E, Vella A, Florio AR, Posteraro P, Perlin DS, Sanguinetti M, Posteraro B. Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species. J Clin Microbiol. 2012;50(7):2479–83.
37. •• Ceyssens PJ, Soetaert K, Timke M, Van den Bossche A, Sparbier K, De Cremer K, Kostrzewa M, Hendrickx M, Mathys V. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for combined species identification and drug sensitivity testing in mycobacteria. J Clin Microbiol. 2017;55(2):624–34. In this paper the simultaneous identification and antifungal susceptibility testing of Mycobacteria by MALDI-TOF MS is described. This is also promising for fungi.
38. Gautier M, Ranque S, Normand AC, Becker P, Packeu A, Cassagne C, L’Ollivier C, Hendrickx M, Piarroux R. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: revolutionizing clinical laboratory diagnosis of mould infections. Clin Microbiol Infect. 2014;20(12):1366–71.
39. Dhieb C, Normand AC, Al-Yasiri M, Chaker E, El Euch D, Vranckx K, Hendrickx M, Sadfi N, Piarroux R, Ranque S. MALDI-TOF typing highlights geographical and fluconazole resistance clusters in Candida glabrata. Med Mycol. 2015;53(5):462–9.
40. Dhieb C, Normand AC, L’Ollivier C, Gautier M, Vranckx K, El Euch D, Chaker E, Hendrickx M, Dalle F, Sadfi N, Piarroux R, Ranque S. Comparison of MALDI-TOF mass spectra with microsatellite length polymorphisms in Candida albicans. J Mass Spectrom. 2015;50(2):371–7.
41. Girard V, Mailler S, Chetry M, Vidal C, Durand G, van Belkum A, Colombo AL, Hagen F, Meis JF, Chowdhary A. Identification and typing of the emerging pathogen Candida auris by matrix-assisted laser desorption ionisation time of flight mass spectrometry. Mycoses. 2016;59(8):535–8.