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Volumn 447, Issue , 2017, Pages 37-46

Thaw-and-use target cells pre-labeled with calcein AM for antibody-dependent cell-mediated cytotoxicity assays

Author keywords

Antibody dependent cell mediated cytotoxicity; Calcein AM; Cell based assay; Cryopreservation; Thaw and use cells

Indexed keywords

CALCEIN; CALCEIN AM; UNCLASSIFIED DRUG; FLUORESCEIN DERIVATIVE; MONOCLONAL ANTIBODY;

EID: 85018656901     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2017.04.005     Document Type: Article
Times cited : (13)

References (50)
  • 1
    • 0031813435 scopus 로고    scopus 로고
    • A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells
    • Allsopp, C.E., Nicholls, S.J., et al. A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells. J. Immunol. Methods 214:1–2 (1998), 175–186.
    • (1998) J. Immunol. Methods , vol.214 , Issue.1-2 , pp. 175-186
    • Allsopp, C.E.1    Nicholls, S.J.2
  • 2
    • 0025821144 scopus 로고
    • Characterization of effector-target conjugates for cloned human natural killer and human lymphokine activated killer cells by flow cytometry
    • Callewaert, D.M., Radcliff, G., et al. Characterization of effector-target conjugates for cloned human natural killer and human lymphokine activated killer cells by flow cytometry. Cytometry, 12, 1991, 666.
    • (1991) Cytometry , vol.12 , pp. 666
    • Callewaert, D.M.1    Radcliff, G.2
  • 3
    • 84914152611 scopus 로고    scopus 로고
    • Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies
    • Cheng, Z.J., Garvin, D., et al. Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies. J. Immunol. Methods 414 (2014), 69–81.
    • (2014) J. Immunol. Methods , vol.414 , pp. 69-81
    • Cheng, Z.J.1    Garvin, D.2
  • 4
    • 50249105179 scopus 로고    scopus 로고
    • Comparative study of four fluorescent probes for evaluation of natural killer cell cytotoxicity assays
    • Cholujova, D., Jakubikova, J., et al. Comparative study of four fluorescent probes for evaluation of natural killer cell cytotoxicity assays. Immunobiology 213:8 (2008), 629–640.
    • (2008) Immunobiology , vol.213 , Issue.8 , pp. 629-640
    • Cholujova, D.1    Jakubikova, J.2
  • 5
    • 84860911340 scopus 로고    scopus 로고
    • Quantitative evaluation of fucose reducing effects in a humanized antibody on Fcgamma receptor binding and antibody-dependent cell-mediated cytotoxicity activities
    • Chung, S., Quarmby, V., et al. Quantitative evaluation of fucose reducing effects in a humanized antibody on Fcgamma receptor binding and antibody-dependent cell-mediated cytotoxicity activities. MAbs 4:3 (2012), 326–340.
    • (2012) MAbs , vol.4 , Issue.3 , pp. 326-340
    • Chung, S.1    Quarmby, V.2
  • 6
    • 84901463516 scopus 로고    scopus 로고
    • Characterization of in vitro antibody-dependent cell-mediated cytotoxicity activity of therapeutic antibodies - impact of effector cells
    • Chung, S., Lin, Y.L., et al. Characterization of in vitro antibody-dependent cell-mediated cytotoxicity activity of therapeutic antibodies - impact of effector cells. J. Immunol. Methods 407 (2014), 63–75.
    • (2014) J. Immunol. Methods , vol.407 , pp. 63-75
    • Chung, S.1    Lin, Y.L.2
  • 7
    • 84910027197 scopus 로고    scopus 로고
    • Multidrug resistance protein 1 (MRP1, ABCC1), a “multitasking” ATP-binding cassette (ABC) transporter
    • Cole, S.P., Multidrug resistance protein 1 (MRP1, ABCC1), a “multitasking” ATP-binding cassette (ABC) transporter. J. Biol. Chem. 289:45 (2014), 30880–30888.
    • (2014) J. Biol. Chem. , vol.289 , Issue.45 , pp. 30880-30888
    • Cole, S.P.1
  • 8
    • 84869777228 scopus 로고    scopus 로고
    • Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies
    • Derer, S., Bauer, P., et al. Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies. J. Immunol. 189:11 (2012), 5230–5239.
    • (2012) J. Immunol. , vol.189 , Issue.11 , pp. 5230-5239
    • Derer, S.1    Bauer, P.2
  • 9
    • 24944514693 scopus 로고    scopus 로고
    • Evaluation of division-arrested cells for cell-based high-throughput screening and profiling
    • Digan, M.E., Pou, C., Niu, H., Zhang, J.-H., Evaluation of division-arrested cells for cell-based high-throughput screening and profiling. J. Biomol. Screen., 10, 2005, 615.
    • (2005) J. Biomol. Screen. , vol.10 , pp. 615
    • Digan, M.E.1    Pou, C.2    Niu, H.3    Zhang, J.-H.4
  • 10
    • 0343306779 scopus 로고    scopus 로고
    • Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein
    • Essodaigui, M., Broxterman, H.J., et al. Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein. Biochemistry 37:8 (1998), 2243–2250.
    • (1998) Biochemistry , vol.37 , Issue.8 , pp. 2243-2250
    • Essodaigui, M.1    Broxterman, H.J.2
  • 11
    • 84964260958 scopus 로고    scopus 로고
    • The modified FACS calcein AM retention assay: a high throughput flow cytometer based method to measure cytotoxicity
    • Gillissen, M.A., Yasuda, E., et al. The modified FACS calcein AM retention assay: a high throughput flow cytometer based method to measure cytotoxicity. J. Immunol. Methods 434 (2016), 16–23.
    • (2016) J. Immunol. Methods , vol.434 , pp. 16-23
    • Gillissen, M.A.1    Yasuda, E.2
  • 12
    • 34948881806 scopus 로고    scopus 로고
    • Increased natural killer cell expression of CD16, augmented binding and ADCC activity to rituximab among individuals expressing the Fc{gamma}RIIIa-158 V/V and V/F polymorphism
    • Hatjiharissi, E., Xu, L., et al. Increased natural killer cell expression of CD16, augmented binding and ADCC activity to rituximab among individuals expressing the Fc{gamma}RIIIa-158 V/V and V/F polymorphism. Blood 110:7 (2007), 2561–2564.
    • (2007) Blood , vol.110 , Issue.7 , pp. 2561-2564
    • Hatjiharissi, E.1    Xu, L.2
  • 13
    • 0028332661 scopus 로고
    • Calcein accumulation as a fluorometric functional assay of the multi-drug transporter
    • Hollo, Z., Homolya, L., et al. Calcein accumulation as a fluorometric functional assay of the multi-drug transporter. Biochim. Biophys. Acta, 1191, 1994, 384.
    • (1994) Biochim. Biophys. Acta , vol.1191 , pp. 384
    • Hollo, Z.1    Homolya, L.2
  • 14
    • 84863270428 scopus 로고    scopus 로고
    • An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells
    • Jang, Y.Y., Cho, D., et al. An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells. Ann. Clin. Lab. Sci. 42:1 (2012), 42–49.
    • (2012) Ann. Clin. Lab. Sci. , vol.42 , Issue.1 , pp. 42-49
    • Jang, Y.Y.1    Cho, D.2
  • 15
    • 1642330455 scopus 로고    scopus 로고
    • New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population
    • Jedema, I., van der Werff, N.M., et al. New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population. Blood 103 (2004), 2677–2682.
    • (2004) Blood , vol.103 , pp. 2677-2682
    • Jedema, I.1    van der Werff, N.M.2
  • 16
    • 34748865088 scopus 로고    scopus 로고
    • Antibody therapeutics: isotype and glycoform selection
    • Jefferis, R., Antibody therapeutics: isotype and glycoform selection. Expert. Opin. Biol. Ther. 7:9 (2007), 1401–1413.
    • (2007) Expert. Opin. Biol. Ther. , vol.7 , Issue.9 , pp. 1401-1413
    • Jefferis, R.1
  • 17
    • 79551575923 scopus 로고    scopus 로고
    • Advances in the assessment and control of the effector functions of therapeutic antibodies
    • Jiang, X.R., Song, A., et al. Advances in the assessment and control of the effector functions of therapeutic antibodies. Nat. Rev. Drug Discov. 10:2 (2011), 101–111.
    • (2011) Nat. Rev. Drug Discov. , vol.10 , Issue.2 , pp. 101-111
    • Jiang, X.R.1    Song, A.2
  • 18
    • 0029996654 scopus 로고    scopus 로고
    • Cytotoxic activity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures of human haematological and solid tumours
    • Jonsson, B., Liminga, G., et al. Cytotoxic activity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures of human haematological and solid tumours. Eur. J. Cancer 32A:5 (1996), 883–887.
    • (1996) Eur. J. Cancer , vol.32A , Issue.5 , pp. 883-887
    • Jonsson, B.1    Liminga, G.2
  • 19
    • 33744940509 scopus 로고    scopus 로고
    • Construction of a conventional non-radioisotope method to quantify HM1.24 antigens: correlation of HM1.24 levels and ADCC activity of the humanized antibody against HM1.24
    • Kawai, S., Koishihara, Y., et al. Construction of a conventional non-radioisotope method to quantify HM1.24 antigens: correlation of HM1.24 levels and ADCC activity of the humanized antibody against HM1.24. Leuk. Res. 30:8 (2006), 949–956.
    • (2006) Leuk. Res. , vol.30 , Issue.8 , pp. 949-956
    • Kawai, S.1    Koishihara, Y.2
  • 20
    • 0030611643 scopus 로고    scopus 로고
    • Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype
    • Koene, H.R., Kleijer, M., et al. Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood 90:3 (1997), 1109–1114.
    • (1997) Blood , vol.90 , Issue.3 , pp. 1109-1114
    • Koene, H.R.1    Kleijer, M.2
  • 21
    • 0023917432 scopus 로고
    • Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF)
    • Kolber, M.A., Quinones, R.R., et al. Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF). J. Immunol. Methods 108 (1988), 255–264.
    • (1988) J. Immunol. Methods , vol.108 , pp. 255-264
    • Kolber, M.A.1    Quinones, R.R.2
  • 22
    • 84964911973 scopus 로고    scopus 로고
    • Comparing high-throughput methods to measure NK cell-mediated antibody dependent cellular cytotoxicity during HIV-infection
    • Konstantinus, I.N., Gamieldien, H., et al. Comparing high-throughput methods to measure NK cell-mediated antibody dependent cellular cytotoxicity during HIV-infection. J. Immunol. Methods 434 (2016), 46–52.
    • (2016) J. Immunol. Methods , vol.434 , pp. 46-52
    • Konstantinus, I.N.1    Gamieldien, H.2
  • 23
    • 17344364488 scopus 로고    scopus 로고
    • Application of division arrest technology to cell-based HTS: comparison with frozen and fresh cells
    • Kunapuli, P., Zheng, W., et al. Application of division arrest technology to cell-based HTS: comparison with frozen and fresh cells. Assay Drug Dev. Technol., 3, 2005, 17.
    • (2005) Assay Drug Dev. Technol. , vol.3 , pp. 17
    • Kunapuli, P.1    Zheng, W.2
  • 24
    • 80054859408 scopus 로고    scopus 로고
    • Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFalpha antagonists
    • Lallemand, C., Kavrochorianou, N., et al. Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFalpha antagonists. J. Immunol. Methods 373:1–2 (2011), 229–239.
    • (2011) J. Immunol. Methods , vol.373 , Issue.1-2 , pp. 229-239
    • Lallemand, C.1    Kavrochorianou, N.2
  • 25
    • 0027935971 scopus 로고
    • CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity
    • Lichtenfels, R., Biddison, W.E., et al. CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity. J. Immunol. Methods 172:2 (1994), 227–239.
    • (1994) J. Immunol. Methods , vol.172 , Issue.2 , pp. 227-239
    • Lichtenfels, R.1    Biddison, W.E.2
  • 26
    • 0029130117 scopus 로고
    • Cytotoxic effect of calcein acetoxymethyl ester on human tumor cell lines: drug delivery by intracellular trapping
    • Liminga, G., P. Nygren P, et al., Cytotoxic effect of calcein acetoxymethyl ester on human tumor cell lines: drug delivery by intracellular trapping. Anti-Cancer Drugs, 6, 1995, 578.
    • (1995) Anti-Cancer Drugs , vol.6 , pp. 578
    • Liminga, G.1    P. Nygren P, et al.,2
  • 27
    • 0025086821 scopus 로고
    • Cytotoxicity assay using cryopreserved target cells pre-labeled with the fluorescent marker europium
    • Maley, D., Simon, P., Cytotoxicity assay using cryopreserved target cells pre-labeled with the fluorescent marker europium. J. Immunol. Methods 134 (1990), 61–70.
    • (1990) J. Immunol. Methods , vol.134 , pp. 61-70
    • Maley, D.1    Simon, P.2
  • 28
    • 84900851173 scopus 로고    scopus 로고
    • Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in (51)Cr-release and CD107a assays
    • Mata, M.M., Mahmood, F., et al. Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in (51)Cr-release and CD107a assays. J. Immunol. Methods 406 (2014), 1–9.
    • (2014) J. Immunol. Methods , vol.406 , pp. 1-9
    • Mata, M.M.1    Mahmood, F.2
  • 29
    • 27244450053 scopus 로고    scopus 로고
    • Trastuzumab-mediated antibody-dependent cellular cytotoxicity against esophageal squamous cell carcinoma
    • Mimura, K., Kono, K., et al. Trastuzumab-mediated antibody-dependent cellular cytotoxicity against esophageal squamous cell carcinoma. Clin. Cancer Res. 11:13 (2005), 4898–4904.
    • (2005) Clin. Cancer Res. , vol.11 , Issue.13 , pp. 4898-4904
    • Mimura, K.1    Kono, K.2
  • 30
    • 0034765888 scopus 로고    scopus 로고
    • Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants
    • Neri, S., Mariani, E., et al. Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants. Clin. Diagn. Lab. Immunol. 8:6 (2001), 1131–1135.
    • (2001) Clin. Diagn. Lab. Immunol. , vol.8 , Issue.6 , pp. 1131-1135
    • Neri, S.1    Mariani, E.2
  • 31
    • 0028566062 scopus 로고
    • An improved fluorescence assay for the determination of lymphocyte-mediated cytotoxicity using flow cytometry
    • Papadopoulos, N.G., Dedoussis, G.V., et al. An improved fluorescence assay for the determination of lymphocyte-mediated cytotoxicity using flow cytometry. J. Immunol. Methods 177:1–2 (1994), 101–111.
    • (1994) J. Immunol. Methods , vol.177 , Issue.1-2 , pp. 101-111
    • Papadopoulos, N.G.1    Dedoussis, G.V.2
  • 32
    • 0029042586 scopus 로고
    • An improved assay for antibody dependent cellular cytotoxicity based on time resolved fluorometry
    • Patel, A.K., Boyd, P.N., An improved assay for antibody dependent cellular cytotoxicity based on time resolved fluorometry. J. Immunol. Methods 184:1 (1995), 29–38.
    • (1995) J. Immunol. Methods , vol.184 , Issue.1 , pp. 29-38
    • Patel, A.K.1    Boyd, P.N.2
  • 33
    • 78650597089 scopus 로고    scopus 로고
    • IgG isotype, glycosylation, and EGFR expression determine the induction of antibody-dependent cellular cytotoxicity in vitro by cetuximab
    • Patel, D., Guo, X., et al. IgG isotype, glycosylation, and EGFR expression determine the induction of antibody-dependent cellular cytotoxicity in vitro by cetuximab. Hum. Antibodies 19:4 (2010), 89–99.
    • (2010) Hum. Antibodies , vol.19 , Issue.4 , pp. 89-99
    • Patel, D.1    Guo, X.2
  • 34
    • 55249121695 scopus 로고    scopus 로고
    • Antibody fucosylation differentially impacts cytotoxicity mediated by NK and PMN effector cells
    • Peipp, M., Lammerts van Bueren, J.J., et al. Antibody fucosylation differentially impacts cytotoxicity mediated by NK and PMN effector cells. Blood 112:6 (2008), 2390–2399.
    • (2008) Blood , vol.112 , Issue.6 , pp. 2390-2399
    • Peipp, M.1    Lammerts van Bueren, J.J.2
  • 35
    • 22744443857 scopus 로고    scopus 로고
    • Rituximab mediates in vitro antileukemic activity in pediatric patients after allogeneic transplantation
    • Pfeiffer, M., Stanojevic, S., et al. Rituximab mediates in vitro antileukemic activity in pediatric patients after allogeneic transplantation. Bone Marrow Transplant. 36:2 (2005), 91–97.
    • (2005) Bone Marrow Transplant. , vol.36 , Issue.2 , pp. 91-97
    • Pfeiffer, M.1    Stanojevic, S.2
  • 36
    • 79960555557 scopus 로고    scopus 로고
    • High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses
    • Pollara, J., Hart, L., et al. High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses. Cytometry A 79:8 (2011), 603–612.
    • (2011) Cytometry A , vol.79 , Issue.8 , pp. 603-612
    • Pollara, J.1    Hart, L.2
  • 37
    • 0021278706 scopus 로고
    • The standardization of NK cell assays for use in studies of biological response modifiers
    • Pross, H.F., Maroun, J.A., The standardization of NK cell assays for use in studies of biological response modifiers. J. Immunol. Methods 68:1–2 (1984), 235–249.
    • (1984) J. Immunol. Methods , vol.68 , Issue.1-2 , pp. 235-249
    • Pross, H.F.1    Maroun, J.A.2
  • 38
    • 48549090941 scopus 로고    scopus 로고
    • Terminal sugars of Fc glycans influence antibody effector functions of IgGs
    • Raju, T.S., Terminal sugars of Fc glycans influence antibody effector functions of IgGs. Curr. Opin. Immunol. 20:4 (2008), 471–478.
    • (2008) Curr. Opin. Immunol. , vol.20 , Issue.4 , pp. 471-478
    • Raju, T.S.1
  • 39
    • 78651376557 scopus 로고    scopus 로고
    • New anti-CD20 monoclonal antibodies for the treatment of B-cell lymphoid malignancies
    • Robak, T., Robak, E., New anti-CD20 monoclonal antibodies for the treatment of B-cell lymphoid malignancies. BioDrugs 25:1 (2011), 13–25.
    • (2011) BioDrugs , vol.25 , Issue.1 , pp. 13-25
    • Robak, T.1    Robak, E.2
  • 40
    • 0032811719 scopus 로고    scopus 로고
    • A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology
    • Roden, M.M., Lee, K.H., et al. A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J. Immunol. Methods 226:1–2 (1999), 29–41.
    • (1999) J. Immunol. Methods , vol.226 , Issue.1-2 , pp. 29-41
    • Roden, M.M.1    Lee, K.H.2
  • 41
    • 79957790057 scopus 로고    scopus 로고
    • Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies
    • Schnueriger, A., Grau, R., et al. Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies. Mol. Immunol. 48:12 − 13 (2011), 1512–1517.
    • (2011) Mol. Immunol. , vol.48 , Issue.12 − 13 , pp. 1512-1517
    • Schnueriger, A.1    Grau, R.2
  • 42
    • 0037178791 scopus 로고    scopus 로고
    • Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity
    • Shields, R.L., J. Lai J, et al., Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J. Biol. Chem. 277 (2002), 26733–26740.
    • (2002) J. Biol. Chem. , vol.277 , pp. 26733-26740
    • Shields, R.L.1    Lai J, J.2
  • 43
    • 0020316560 scopus 로고
    • Cryopreservation of human mononuclear cells for quality control in clinical immunology. I. Correlations in recovery of K- and NK-cell functions, surface markers, and morphology
    • Strong, D.M., Ortaldo, J.R., et al. Cryopreservation of human mononuclear cells for quality control in clinical immunology. I. Correlations in recovery of K- and NK-cell functions, surface markers, and morphology. J. Clin. Immunol. 2:3 (1982), 214–221.
    • (1982) J. Clin. Immunol. , vol.2 , Issue.3 , pp. 214-221
    • Strong, D.M.1    Ortaldo, J.R.2
  • 44
    • 79960031654 scopus 로고    scopus 로고
    • Increased consistency and efficiency in routine potency testing by bioassay with direct use of cryopreserved (ready-to-plate) cells
    • TerWee, J.A., Chin, C.L., et al. Increased consistency and efficiency in routine potency testing by bioassay with direct use of cryopreserved (ready-to-plate) cells. J. Immunol. Methods 370:1–2 (2011), 65–74.
    • (2011) J. Immunol. Methods , vol.370 , Issue.1-2 , pp. 65-74
    • TerWee, J.A.1    Chin, C.L.2
  • 45
    • 0027489622 scopus 로고
    • A new microcellular cytotoxicity test based on calcein AM release
    • Wang, X.M., Terasaki, P.I., et al. A new microcellular cytotoxicity test based on calcein AM release. Hum. Immunol. 37:4 (1993), 264–270.
    • (1993) Hum. Immunol. , vol.37 , Issue.4 , pp. 264-270
    • Wang, X.M.1    Terasaki, P.I.2
  • 46
    • 68149137514 scopus 로고    scopus 로고
    • Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization
    • Weinberg, A., Song, L.Y., et al. Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization. Clin. Vaccine Immunol. 16:8 (2009), 1176–1186.
    • (2009) Clin. Vaccine Immunol. , vol.16 , Issue.8 , pp. 1176-1186
    • Weinberg, A.1    Song, L.Y.2
  • 48
    • 84945454142 scopus 로고    scopus 로고
    • A neutralizing antibody assay based on a reporter of antibody-dependent cell-mediated cytotoxicity
    • Wu, Y., Li, J.J., et al. A neutralizing antibody assay based on a reporter of antibody-dependent cell-mediated cytotoxicity. AAPS J. 17:6 (2015), 1417–1426.
    • (2015) AAPS J. , vol.17 , Issue.6 , pp. 1417-1426
    • Wu, Y.1    Li, J.J.2
  • 49
    • 34447268009 scopus 로고    scopus 로고
    • Cryopreserved cells facilitate cell-based drug discovery
    • Zaman, G.J.R., de Roos, J.A.D.M., et al. Cryopreserved cells facilitate cell-based drug discovery. Drug Discov. Today, 12, 2007, 521.
    • (2007) Drug Discov. Today , vol.12 , pp. 521
    • Zaman, G.J.R.1    de Roos, J.A.D.M.2
  • 50
    • 0030895497 scopus 로고    scopus 로고
    • Comparison of europium and chromium release assays: cytotoxicity in healthy individuals and patients with cervical carcinoma
    • von Zons, P., Crowley-Nowick, P., et al. Comparison of europium and chromium release assays: cytotoxicity in healthy individuals and patients with cervical carcinoma. Clin. Diagn. Lab. Immunol. 4:2 (1997), 202–207.
    • (1997) Clin. Diagn. Lab. Immunol. , vol.4 , Issue.2 , pp. 202-207
    • von Zons, P.1    Crowley-Nowick, P.2


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