The use of detergents to purify membrane proteins

Current Protocols in Protein Science

Volumn 2016, Issue , 2016, Pages 4.8.1-4.8.35

  Orwick Rydmark, Marcella ^a   Arnold, Thomas ^b   Linke, Dirk ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b Boehringer Ingelheim Veterinary Research Center GmbH and Co KG   (Germany)

Author keywords

Cloud point; Detergent; Detergent exchange; Detergent removal; Membrane protein micelle; Phase separation; Solubilization

Indexed keywords

ACETONE; BIOBEAD; BIOMATERIAL; BUFFER; DETERGENT; MEMBRANE PROTEIN; PYRENE; TRITON X 114; UNCLASSIFIED DRUG; MACROGOL DERIVATIVE; MICELLE; NONIDET P-40;

AFFINITY CHROMATOGRAPHY; ANTIBODY PRODUCTION; ARTICLE; BIOCOMPATIBILITY; CIRCULAR DICHROISM; CRITICAL MICELLE CONCENTRATION; CRYSTALLOGRAPHY; ELECTRON MICROSCOPY; ELECTROPHORESIS; ENZYMATIC ASSAY; ESCHERICHIA COLI; FLUORESCENCE; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE; IONIC STRENGTH; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; OPTICAL SPECTROSCOPY; PHASE SEPARATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEOMICS; SOLUBILITY; SOLUBILIZATION; SPECTROSCOPY; TEMPERATURE; BIOCHEMISTRY; CHEMISTRY; CHROMATOGRAPHY; COLORIMETRY; DIALYSIS; ISOLATION AND PURIFICATION; MICELLE; OSMOLARITY; PROCEDURES;

BIOCHEMISTRY; BUFFERS; CHROMATOGRAPHY; COLORIMETRY; CRYSTALLOGRAPHY; DETERGENTS; DIALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; MEMBRANE PROTEINS; MICELLES; MICROSCOPY, ELECTRON; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OSMOLAR CONCENTRATION; POLYETHYLENE GLYCOLS; SOLUBILITY;

EID: 85018487705     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0408s84     Document Type: Article

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