Assaying spontaneous network activity and cellular viability using multi-well microelectrode arrays

Methods in Molecular Biology

Volumn 1601, Issue , 2017, Pages 153-170

  Brown, Jasmine P ^a   Lynch, Brittany S ^a   Curry Chisolm, Itaevia M ^a   Shafer, Timothy J ^a   Strickland, Jenna D ^b,c  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b AXION BIOSYSTEMS   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Developmental screening; Microelectrode array (MEA); mwMEA; Neurophysiological method

Indexed keywords

BRAIN NERVE CELL; CELL VIABILITY; CELL VIABILITY ASSAY; CONTROLLED STUDY; CYTOTOXICITY ASSAY; DEVELOPMENTAL SCREENING; FLUORESCENCE; LAMINAR FLOW; MICROELECTRODE; MULTI WELL MICROELECTRODE ARRAY; NERVE CELL NETWORK; PH; ANIMAL; CELL SURVIVAL; CYTOLOGY; DEVICES; DRUG EFFECTS; LONG EVANS RAT; MICROARRAY ANALYSIS; NEOCORTEX; NERVE CELL; PRIMARY CELL CULTURE; RAT; TOXICITY TESTING;

CYTOTOXIN;

ANIMALS; CELL SURVIVAL; CYTOTOXINS; MICROARRAY ANALYSIS; MICROELECTRODES; NEOCORTEX; NERVE NET; NEURONS; PRIMARY CELL CULTURE; RATS; RATS, LONG-EVANS; TOXICITY TESTS;

EID: 85018424306     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6960-9_13     Document Type: Chapter

References (7)

1. Valdivia P, Martin MT, Houck K, Lefew WR, Ross J, Shafer TJ (2014) Multi-well microelectrode array recordings detect neuroactivity of ToxCast compounds. Neurotoxicology 44:204-217
2. Nicolas J, Hendriksen PJM, van Kleef RGDM, de Groot A, Bovee TFH, Rietjens IMCM, Westerink RHS (2014) Detection of marine neurotoxins in food safety testing using a multielectrode array. Mol Nutr Food Res 58: 2369-2378
3. Hogberg HT, Sobanski T, Novellino A, Whelan M, Weiss DG, Bal-Price AK (2011) Application of micro-electrode arrays (MEAs) as an emerging technology for developmental neurotoxicity: evaluation of domoic acid-induced effects in primary cultures of rat cortical neurons. Neurotoxicology 32:158-168
4. Robinette BL, Harrill JA, Mundy WR, Shafer TJ (2011) In vitro assessment of developmental neurotoxicity: use of microelectrode arrays to measure functional changes in neuronal network ontogeny. Front Neuroeng 4:1
5. Cotterill E, Hall D, Wallace K, Mundy WR, Eglen SJ, Shafer TJ (2016) Characterization of early cortical neural network development in multiwell microelectrode array plates. J Biomol Screen 21(5):510-519
6. Johnstone AF, Gross GW, Weiss DG, Schroeder OH, Gramowski A, Shafer TJ (2010) Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology 31:331-350
7. Wallace K, Strickland JD, Valdivia P, Mundy WR, Shafer TJ (2015) A multiplexed assay for determination of neurotoxicant effects on spontaneous network activity and viability from microelectrode arrays. Neurotoxicology 49: 79-85