Isolation and characterization of exosomes from cultures of tissue explants and cell lines

Current Protocols in Immunology

Volumn 2016, Issue , 2016, Pages 14.42.1-14.42.21

  Mincheva Nilsson, Lucia ^a   Baranov, Vladimir ^a   Nagaeva, Olga ^a   Dehlin, Eva ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

density ultracentrifugation; exosomes; Extracellular vesicles; flow cytometry; negative contrast staining; Organ explant culture; sucrose gradient; TEM

Indexed keywords

CELL SURFACE PROTEIN; BIOLOGICAL MARKER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CELL LINE; CULTURE MEDIUM; EMBEDDING; EXOSOME; EXPLANT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOELECTRON MICROSCOPY; MATERIAL COATING; NONHUMAN; PRIORITY JOURNAL; STAINING; STRATEGIC PLANNING; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TISSUE CULTURE; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; CELL CULTURE; CELL FRACTIONATION; CHEMISTRY; CONDITIONED MEDIUM; DENSITY GRADIENT CENTRIFUGATION; IMMUNOPHENOTYPING; METABOLISM; PROCEDURES; TISSUE CULTURE TECHNIQUE; ULTRACENTRIFUGATION;

ANIMALS; BIOMARKERS; CELL FRACTIONATION; CELL LINE; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CULTURE MEDIA, CONDITIONED; EXOSOMES; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; STAINING AND LABELING; TISSUE CULTURE TECHNIQUES; ULTRACENTRIFUGATION;

EID: 85018275366     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/cpim.17     Document Type: Article

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