Live Cell Imaging: Assessing the Phototoxicity of 488 and 546 nm Light and Methods to Alleviate it

Journal of Cellular Physiology

Volumn 232, Issue 9, 2017, Pages 2461-2468

  Douthwright, Stephen ^a   Sluder, Greenfield ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P21; PROTEIN P53; REACTIVE OXYGEN METABOLITE; 6-HYDROXY-2,5,7,8-TETRAMETHYLCHROMAN-2-CARBOXYLIC ACID; ANTIOXIDANT; CALCIUM BINDING PROTEIN; CELL CYCLE PROTEIN; CETN1 PROTEIN, HUMAN; CHROMAN DERIVATIVE; GREEN FLUORESCENT PROTEIN; OXYGEN; PHOTOPROTEIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; BLUE LIGHT; CANCER CELL LINE; CELL CYCLE ARREST; CELL CYCLE CHECKPOINT; CELL DEATH; CELL POPULATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; GREEN LIGHT; HUMAN; HUMAN CELL; IMAGING; LIGHT; LIGHT EXPOSURE; LIGHT INTENSITY; LIVE CELL IMAGING; MITOSIS; MITOSIS INHIBITION; NONHUMAN; OXIDATIVE STRESS; PHOTOTOXICITY; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PROTEIN EXPRESSION; RADIATION DOSE; VIDEO MICROSCOPY; ADVERSE EFFECTS; BIOSYNTHESIS; CELL LINE; CELL PROTECTION; DRUG EFFECTS; EPITHELIUM CELL; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; METABOLISM; PATHOLOGY; RADIATION RESPONSE; RETINAL PIGMENT EPITHELIUM; TIME FACTOR;

ANTIOXIDANTS; CALCIUM-BINDING PROTEINS; CELL CYCLE CHECKPOINTS; CELL CYCLE PROTEINS; CELL LINE; CHROMANS; CYTOPROTECTION; DOSE-RESPONSE RELATIONSHIP, RADIATION; EPITHELIAL CELLS; GREEN FLUORESCENT PROTEINS; HUMANS; LIGHT; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MITOSIS; OXIDATIVE STRESS; OXYGEN; RECOMBINANT PROTEINS; RETINAL PIGMENT EPITHELIUM; TIME FACTORS; TRANSFECTION;

EID: 85017639162     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.25588     Document Type: Article

References (23)

1. Cardullo RA. 2013. Theoretical principles and practical considerations for fluorescence resonance energy transfer microscopy. Methods Cell Biol 114:441–456.
2. Carlton PM, Boulanger J, Kervrann C, Sibarita JB, Salamero J, Gordon-Messer S, Bresson D, Haber JE, Haase S, Shao L, Winoto L, Matsudea A, Kner P, Uzawa S, Gustafsson M, Kan Z, Agard DA, Sedat JW. 2010. Fast live simultaneous multiwavelength four-dimensional optical microscopy. Proc Natl Acad Sci USA 107:16016–16022.
3. Carreau A, Hafny-Rahbi BE, Matejuk A, Grillon C, Kieda C. 2011. Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia. J Cell Mol Med 15:1239–1253.
4. Chen LC, Lloyd WR, Chang CW, Sud D, Mycek MA. 2013. Fluorescence lifetime imaging microscopy for quantitative biological imaging. Methods Cell Biol 14:457–488.
5. Dixit R, Cyr R. 2003. Cell damage and reactive oxygen species production induced by fluorescence microscopy: Effect on mitosis and guidelines for non-invasive fluorescence microscopy. Plant J 36:280–290.
6. Hinchcliffe EH, Sluder G. 2001. “ It takes two to tango”: Understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev 15:1167–1181.
7. Hoebe RA, Van Oven CH, Gadella TWJ, Dhonukshe PB, Van Noorden JF, Manders EMM. 2007. Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging. Nat Biotechnol 25:249–253.
8. Khodjakov A, Rieder CL. 1999. The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. J Cell Biol 146:585–596.
9. Khodjakov A, Rieder CL. 2001. Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. J Cell Biol 153:237–242.
10. Kuse Y, Ogawa K, Tsuruma K, Shimazawa M, Hara H. 2014. Damage of photoreceptor-derived cells in culture induced by light emitting diode-derived blue light. Sci Rep 4:5223.
11. Magidson V, Khodjakov A. 2013. Circumventing photodamage in live-cell microscopy. Methods Cell Biol 114:545–560.
12. O'Connor N, Silver RB. 2013. Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol 114:387–406.
13. Piel M, Nordberg J, Euteneuer U, Bornens M. 2001. Centrosome-dependent exit of cytokinesis in animal cells. Science 291:1550–1553.
14. Sluder G, Nordberg JJ, Miller FJ, Hinchcliffe EH. 2004. A sealed preparation for long-term observations of cultured cells. In: Spector DL, Goldman RD, editors. Live cell imaging: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp 345–349.
15. Stephens D, Allan VJ. 2003. Light microscopy techniques for live cell imaging. Science 300:82–86.
16. Swedlow JR, Andrews PD. 2005. In vivo imaging of mammalian cells. In: Spector DL, Goldman RD, editors. Live cell imaging: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp 329–343.
17. Tinevez JY, Dragavon J, Baba-Aissa L, Roux P, Perret E, Canivet A, Galy V, Shorte S. 2012. A quantitative method for measuring phototoxicity of a live cell imaging microscope. Methods Enzymol 506:291–309.
18. Uetake Y, Sluder G. 2004. Cell cycle progression after cleavage failure: Mammalian somatic cells do not possess a “tetraploidy checkpoint”. J Cell Biol 165:609–615.
19. Uetake Y, Sluder G. 2010. Prolonged prometaphase blocks daughter cell proliferation despite normal completion of mitosis. Curr Biol 20:1666–1671.
20. Uetake Y, Loncarek J, Nordberg JJ, English CN, La Terra S, Khodjakov A, Sluder G. 2007. Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells. J Cell Biol 176:173–182.
21. Waters J. 2013. Live-cell fluorescence imaging. Methods Cell Biol 14:125–150.
22. Zdolsek JM. 1993. Acridine orange-mediated photodamage to cultured cells. APMIS 101:127.
23. Zdolsek JM, Olsson GM, Brunk UT. 1990. Photooxidative damage to lysosomes of cultured macrophages by acridine orange. Photochem Photobiol 51:67–76.