A quantitative method for measuring phototoxicity of a live cell imaging microscope

Methods in Enzymology

Volumn 506, Issue , 2012, Pages 291-309

  Tinevez, Jean Yves ^a   Dragavon, Joe ^a   Baba Aissa, Lamya ^a   Roux, Pascal ^a   Perret, Emmanuelle ^a   Canivet, Astrid ^a   Galy, Vincent ^b   Shorte, Spencer ^a  

^a INSTITUT PASTEUR   (France)

^b Sorbonne Universités   (France)

Author keywords

Keywords; Live imaging; Phototoxicity

Indexed keywords

ARTICLE; ARTIFACT; BLEACHING; CELL VIABILITY; FLUORESCENCE; HOST PATHOGEN INTERACTION; HUMAN; ILLUMINATION; LIGHT EXPOSURE; MAMMAL CELL; MEASUREMENT; MICROSCOPY; NONHUMAN; PHOTOTOXICITY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RISK ASSESSMENT;

EID: 84857337380     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-391856-7.00039-1     Document Type: Chapter

References (29)

1. R. Amino Quantitative imaging of Plasmodium transmission from mosquito to mammal Nat. Med 12 2006 220 224 (Pubitemid 43214749)
2. R. Amino Imaging malaria sporozoites in the dermis of the mammalian host Nat.Protoc. 2 2007 1705 1712
3. N. Arhel Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes Nat. Methods 3 2006 817 824 (Pubitemid 44445829)
4. Z. Bao Automated cell lineage tracing in Caenorhabditis elegans Proc. Natl. Acad. Sci. USA 103 8 2006 2707 2712
5. Z. Bao Control of cell cycle timing during C. elegans embryogenesis Dev. Biol. 318 1 2008 65 72
6. C.D. Bortner, and J.A. Cidlowski Apoptotic volume decrease and the incredible shrinking cell Cell Death Differ. 9 12 2002 1307 1310 (Pubitemid 36097700)
7. W.H. De Vos Controlled light exposure microscopy reveals dynamic telomere microterritories throughout the cell cycle Cytometry A 75 5 2009 428 439
8. A. Diaspro Photobleaching J.B. Pawley, Handbook of Biological Confocal Microscopy 3rd edn 2006 690 702
9. R. Dixit, and R. Cyr Cell damage and reactive oxygen species production induced by fluorescence microscopy: Effect on mitosis and guidelines for non-invasive fluorescence microscopy Plant J. 36 2 2003 280 290 (Pubitemid 37293067)
10. J. Enninga Secretion of type III effectors into host cells in real time Nat. Methods 2 12 2005 959 965 (Pubitemid 43108733)
11. F. Frischknecht, and S. Shorte Imaging host-pathogen interactions Biotechnol. J. (Special Edition) 4 6 2009 773 948 Wiley-Blackwell
12. K. Gousset Prions hijack tunnelling nanotubes for intercellular spread Nat. Cell Biol. 3 2009 328 336
13. R.A. Hoebe Quantitative determination of the reduction of phototoxicity and photobleaching by controlled light exposure microscopy J. Microsc. 231 1 2008 9 20 (Pubitemid 351951877)
14. S. Inoué, and T. Inoué Direct-view high-speed confocal scanner: The CSU-10 Methods Cell Biol. 70 2002 87 127 (Pubitemid 44403529)
15. N. Ji High-speed, low-photodamage nonlinear imaging using passive pulse splitters Nat. Methods 5 2 2008 197 202 (Pubitemid 351192665)
16. N. Kimble, and D. Hirsh The postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans Dev. Biol. 70 2 1979 396 417 (Pubitemid 10223307)
17. H.J. Koester Ca2 + fluorescence imaging with pico- and femtosecond two-photon excitation: Signal and photodamage Biophys. J. 77 4 1999 2226 2236 (Pubitemid 29463183)
18. K. König Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes Opt. Lett. 24 2 1999 113 115 (Pubitemid 129309658)
19. T. Nishigaki Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging Bio Techniques 41 2 2006 191 197 (Pubitemid 46542603)
20. V. Praitis Creation of low-copy integrated transgenic lines in Caenorhabditis elegans Genetics 157 3 2001 1217 1226 (Pubitemid 32225014)
21. R.K. Saetzler Intravital fluorescence microscopy: Impact of light-induced phototoxicity on adhesion of fluorescently labeled leukocytes J. Histochem. Cytochem. 45 4 1997 505 513 (Pubitemid 27175795)
22. S. Shorte, and F Frischknecht Imaging Cellular and Molecular Biological Functions, Springer-Verlag 2008 Heidelberg Germany
23. J.E. Sulston, and H.R. Horvitz Post-embryonic cell lineages of the nematode, Caenorhabditis elegans Dev. Biol. 56 1 1977 110 156 (Pubitemid 8049332)
24. The Caenorhabditis elegans Genetics Center page on AZ212 strain. https://cgcdb.msi.umn.edu/strain.php?id=5544
25. S. Thiberge In vivo imaging of malaria parasites in the murine liver Nat. Protoc. 2 2007 1811 1818
26. J. Vermot Fast fluorescence microscopy for imaging the dynamics of embryonic development HFSP J. 2 3 2008 143 155
27. M. Wagner Light dose is a limiting factor to maintain cell viability in fluorescence microscopy and single molecule detection Int. J. Mol. Sci. 11 3 2010 956 966
28. J.M. Zdolsek Acridine orange-mediated photodamage to cultured cells APMIS 101 2 1993 127 132 (Pubitemid 23135207)
29. J.M. Zdolsek Photooxidative damage to lysosomes of cultured macrophages by acridine orange Photochem. Photobiol. 51 1 1990 67 76