The cervicovaginal microbiota in women notified for chlamydia trachomatis infection: A case-control study at the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands

Clinical Infectious Diseases

Volumn 64, Issue 1, 2017, Pages 24-31

  Van Der Veer, Charlotte ^a   Bruisten, Sylvia M ^a   Van Der Helm, Jannie J ^b   De Vries, Henry J C ^a,b   Van Houdt, Robin ^c  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b DEPARTMENT OF INFECTIOUS DISEASES   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cervicovaginal microbiota; Chlamydia trachomatis; Contact tracing

Indexed keywords

RNA 16S;

ADULT; ANAEROBIC BACTERIUM; ARTICLE; CASE CONTROL STUDY; CHLAMYDIASIS; CONTACT EXAMINATION; CONTROLLED STUDY; FEMALE; HUMAN; LACTOBACILLUS CRISPATUS; MAJOR CLINICAL STUDY; NETHERLANDS; NONHUMAN; OUTPATIENT DEPARTMENT; PRIORITY JOURNAL; RNA SEQUENCE; SEXUALLY TRANSMITTED DISEASE; VAGINA FLORA; VAGINA SMEAR; YOUNG ADULT; CHLAMYDIA TRACHOMATIS; CLASSIFICATION; DISEASE NOTIFICATION; GENETICS; HEALTH SURVEY; HIGH THROUGHPUT SEQUENCING; MICROBIOLOGY; MICROFLORA; PHYLOGENY; RISK FACTOR; SEXUAL BEHAVIOR; UTERINE CERVIX; VAGINA;

ADULT; CASE-CONTROL STUDIES; CERVIX UTERI; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DISEASE NOTIFICATION; FEMALE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MICROBIOTA; NETHERLANDS; PHYLOGENY; PUBLIC HEALTH SURVEILLANCE; RISK FACTORS; RNA, RIBOSOMAL, 16S; SEXUAL BEHAVIOR; SEXUALLY TRANSMITTED DISEASES; VAGINA; YOUNG ADULT;

EID: 85015238727     PISSN: 10584838     EISSN: 15376591     Source Type: Journal    
DOI: 10.1093/cid/ciw586     Document Type: Article

References (40)

1. World Health Organization. Global incidence and prevalence of selected curable sexually transmitted infections-2008. Geneva, Switzerland: WHO, 2012.
2. van Aar F, de Moraes M, Morre SA, et al. Chlamydia trachomatis IgG seroprevalence in the general population of the Netherlands in 1996 and in 2007: differential changes by gender and age. Sex Transm Infect 2014; 90:434-40.
3. Brotman RM, Klebanoff MA, Nansel TR, et al. Bacterial vaginosis assessed by gram stain and diminished colonization resistance to incident gonococcal, chlamydial, and trichomonal genital infection. J Infect Dis 2010; 202:1907-15.
4. Martin HL, Richardson BA, Nyange PM, et al. Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J Infect Dis 1999; 180:1863-8.
5. Wiesenfeld HC, Hillier SL, Krohn MA, Landers DV, Sweet RL. Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin Infect Dis 2003; 36:663-8.
6. Ravel J, Gajer P, Abdo Z, et al. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci U S A 2011; 108(suppl 1):4680-7.
7. van de Wijgert JH, Borgdorff H, Verhelst R, et al. The vaginal microbiota: what have we learned after a decade of molecular characterization? PLoS One 2014; 9:e105998-6008.
8. Borgdorff H, Tsivtsivadze E, Verhelst R, et al. Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women. ISME J 2014; 8:1781-93.
9. Gautam R, Borgdorff H, Jespers V, et al. Correlates of the molecular vaginal microbiota composition of African women. BMC Infect Dis 2015; 15:86.
10. Ravel J, Brotman RM, Gajer P, et al. Daily temporal dynamics of vaginal microbiota before, during and after episodes of bacterial vaginosis. Microbiome 2013; 1:29.
11. Srinivasan S, Hoffman NG, Morgan MT, et al. Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria. PLoS One 2012; 7:e37818-33.
12. van Houdt R, Ma B, Speksnijder A, van der Helm JJ, de Vries HJC, Ravel J. The vaginal microbiome as a predictor for acquiring Chlamydia trachomatis. International Human Microbiome Consortium, 2015. Abstract #:Evi-039-142.
13. Masese L, Baeten JM, Richardson BA, et al. Incidence and correlates of Chlamydia trachomatis infection in a high-risk cohort of Kenyan women. Sex Transm Dis 2013; 40:221-5.
14. Ness RB, Kip KE, Soper DE, et al. Bacterial vaginosis (BV) and the risk of incident gonococcal or chlamydial genital infection in a predominantly black population. Sex Transm Dis 2005; 32:413-7.
15. Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 1991; 29:297-301.
16. Fadrosh DW, Ma B, Gajer P, et al. An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform. Microbiome 2014; 2:6-13.
17. Bolger AM, Lohse M, Usadel B. Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114-20.
18. Masella AP, Bartram AK, Truszkowski JM, Brown DG, Neufeld JD. PANDAseq: paired-end assembler for Illumina sequences. BMC Bioinformatics 2012; 13: 31-8.
19. Schmieder R, Lim YW, Rohwer F, Edwards R. TagCleaner: identification and removal of tag sequences from genomic and metagenomic datasets. BMC Bioinformatics 2010; 11:341-55.
20. Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of highthroughput community sequencing data. Nat Methods 2010; 7:335-6.
21. Caporaso JG, Bittinger K, Bushman FD, De Santis TZ, Andersen GL, Knight R. PyNAST: A flexible tool for aligning sequences to a template alignment. Bioinformatics 2010; 26:266-7.
22. Matsen FA, Kodner RB, Armbrust EV. PPLACER: linear time maximumlikelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree. BMC Bioinformatics 2010; 11:538-54.
23. R Foundation for Statistical Computing. R: A language and environment for statistical computing. Vienna, Austria: R Foundation, 2014.
24. Wickham H. Ggplot2: elegant graphics for data analysis. New York: Springer, 2009.
25. Shrier I, Platt RW. Reducing bias through directed acyclic graphs. BMC Med Res Methodol 2008; 8:70-85.
26. Segata N, Izard J, Waldron L, et al. Metagenomic biomarker discovery and explanation. Genome Biol 2011; 12:R60-R78.
27. Molano M, Meijer CJ, Weiderpass E, et al. The natural course of Chlamydia trachomatis infection in asymptomatic Colombian women: A 5-year follow-up study. J Infect Dis 2005; 191:907-16.
28. Morre SA, van den Brule AJ, Rozendaal L, et al. The natural course of asymptomatic Chlamydia trachomatis infections: 45% clearance and no development of clinical PID after one-year follow-up. Int J STD AIDS 2002; 13(suppl 2):12-8.
29. Gallo MF, Macaluso M, Warner L, et al. Bacterial vaginosis, gonorrhea, and chlamydial infection among women attending a sexually transmitted disease clinic: A longitudinal analysis of possible causal links. Ann Epidemiol 2012; 22:213-20.
30. Abbai NS, Reddy T, Ramjee G. Prevalent bacterial vaginosis infection-a risk factor for incident sexually transmitted infections in women in Durban, South Africa [manuscript published online ahead of print]. Int J STD AIDS 2015.
31. Borgdorff H, Gautam R, Armstrong SD, et al. Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier. Mucosal Immunol 2015; 9:621-33.
32. Witkin SS, Mendes-Soares H, Linhares IM, Jayaram A, Ledger WJ, Forney LJ. Influence of vaginal bacteria and D- And L-lactic acid isomers on vaginal extracellular matrix metalloproteinase inducer: implications for protection against upper genital tract infections. MBio 2013; 4:e00460-13.
33. Kyongo JK, Crucitti T, Menten J, et al. Cross-sectional analysis of selected genital tract immunological markers and molecular vaginal microbiota in sub-Saharan African women, with relevance to HIV risk and prevention. Clin Vaccine Immunol 2015; 22:526-38.
34. Rizzo A, Fiorentino M, Buommino E, Donnarumma G, Losacco A, Bevilacqua N. Lactobacillus crispatus mediates anti-inflammatory cytokine interleukin-10 induction in response to Chlamydia trachomatis infection in vitro. Int J Med Microbiol 2015; 305:815-27.
35. Gajer P, Brotman RM, Bai G, et al. Temporal dynamics of the human vaginal microbiota. Sci Transl Med 2012; 4:132-52.
36. Macklaim JM, Fernandes AD, Di Bella JM, Hammond JA, Reid G, Gloor GB. Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis. Microbiome 2013; 1:12-33.
37. Borgdorff H, Verwijs MC, Wit FW, et al. The impact of hormonal contraception and pregnancy on sexually transmitted infections and on cervicovaginal microbiota in African sex workers. Sex Transm Dis 2015; 42:143-52.
38. Brotman RM, Klebanoff MA, Nansel TR, et al. A longitudinal study of vaginal douching and bacterial vaginosis-a marginal structural modeling analysis. Am J Epidemiol 2008; 168:188-96.
39. Jespers V, van de Wijgert J, Cools P, et al. The significance of Lactobacillus crispatus and L. vaginalis for vaginal health and the negative effect of recent sex: A cross-sectional descriptive study across groups of African women. BMC Infect Dis 2015; 15:115.
40. Borgdorff H, van der Veer C, van Houdt R, et al. Women of Dutch ethnic origin have lower prevalence of vaginal microbiota dysbiosis than women of other ethnic origins residing in Amsterdam-the HELIUS study. In: European Congress of Clinical Microbiology and Infectious Diseases, Amsterdam, the Netherlands, 9- 12 April 2016. Abstract O474.