Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins

FEBS Journal

Volumn 284, Issue 7, 2017, Pages 1056-1069

  Bence, Melinda ^a   Jankovics, Ferenc ^a   Lukácsovich, Tamás ^b   Erdélyi, Miklós ^a  

^a INSTITUTE OF BIOCHEMISTRY   (Hungary)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

auxin inducible degradation; CRISPR Cas9; Drosophila melanogaster; induced protein depletion; Vasa

Indexed keywords

MESSENGER RNA; RNA BINDING PROTEIN; UNCLASSIFIED DRUG; VASA PROTEIN; DEAD BOX PROTEIN; DROSOPHILA PROTEIN; GURKEN PROTEIN, DROSOPHILA; INDOLEACETIC ACID DERIVATIVE; ISOPROTEIN; TRANSFORMING GROWTH FACTOR ALPHA; VAS PROTEIN, DROSOPHILA;

ADULT; ALTERNATIVE RNA SPLICING; AUXIN INDUCIBLE DEGRADATION SYSTEM; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DROSOPHILA MELANOGASTER; EDITORIAL; EMBRYO; FEMALE; GENE EDITING; LOSS OF FUNCTION MUTATION; MALE; NONHUMAN; OOCYTE; PHENOTYPE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; ALLELE; ANIMAL; CELL POLARITY; CYTOLOGY; DEFICIENCY; DOSE RESPONSE; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; GENOTYPE; GROWTH, DEVELOPMENT AND AGING; MATERNAL INHERITANCE; METABOLISM; NONMAMMALIAN EMBRYO; OOCYTE DEVELOPMENT; OVARY; PROCEDURES; PROTEIN SYNTHESIS;

ALLELES; ANIMALS; CELL POLARITY; CRISPR-CAS SYSTEMS; DEAD-BOX RNA HELICASES; DOSE-RESPONSE RELATIONSHIP, DRUG; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; EMBRYO, NONMAMMALIAN; FEMALE; GENE EDITING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENOTYPE; INDOLEACETIC ACIDS; MATERNAL INHERITANCE; OOCYTES; OOGENESIS; OVARY; PHENOTYPE; PROTEIN BIOSYNTHESIS; PROTEIN ISOFORMS; PROTEOLYSIS; TRANSFORMING GROWTH FACTOR ALPHA;

EID: 85014793754     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.14042     Document Type: Article

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