Recombination-Mediated Genetic Engineering of Large Genomic DNA Transgenes

Methods in Molecular Biology

Volumn 772, Issue , 2011, Pages 445-458

  Ejsmont, Radoslaw Kamil ^a   Ahlfeld, Peter ^a   Pozniakovsky, Andrei ^a   Stewart, A Francis ^a   Tomancak, Pavel ^a   Sarov, Mihail ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

Author keywords

FlyFos; Recombineering; Red ET

Indexed keywords

DNA;

ANIMAL; ARTICLE; DROSOPHILA; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC ENGINEERING; GENETIC MARKER; GENETIC TRANSFORMATION; GENETICS; GENOME; HOMOLOGOUS RECOMBINATION; ISOLATION AND PURIFICATION; METHODOLOGY; PLASMID; REPRODUCIBILITY; TRANSGENE;

ANIMALS; DNA; DROSOPHILA; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC VECTORS; GENOME; HOMOLOGOUS RECOMBINATION; MUTAGENESIS, INSERTIONAL; PLASMIDS; REPRODUCIBILITY OF RESULTS; TRANSFORMATION, GENETIC; TRANSGENES;

EID: 84857226478     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-228-1_26     Document Type: Chapter

References (12)

1. Zhang Y, Buchholz F, Muyrers JP et al (1998) A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20:123–128
2. Murphy KC (1998) Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J Bacteriol 180:2063–2071
3. Muyrers JP, Zhang Y, Testa G et al (1999) Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res 27:1555–1557
4. Testa G, Zhang Y, Vintersten K et al (2003) Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles. Nat Biotechnol 21:443–447
5. Wang J, Sarov M, Rientjes J et al (2006) An improved recombineering approach by adding RecA to lambda Red recombination. Mol Biotechnol 32:43–53
6. Sarov M, Schneider S, Pozniakovski A et al (2006) A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods 3:839–844
7. Poser I, Sarov M, Hutchins JRA et al (2008) BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods 5:409–415
8. Ejsmont RK, Sarov M, Winkler S et al (2009) A toolkit for high-throughput, cross-species gene engineering in Drosophila. Nat Methods 6:435–437
9. Groth AC, Fish M, Nusse R et al (2004) Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166:1775–1782
10. Sharma RC, Schimke RT (1996) Preparation of electrocompetent E coli using salt-free growth medium. Biotechniques 20:42–44
11. Maresca M, Erler A, Fu J et al (2010) Single-stranded heteroduplex intermediates in lambda Red homologous recombination. BMC Mol Biol 11:54
12. Bischof J, Maeda RK, Hediger M et al (2007) An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc Natl Acad Sci USA 104: 3312–3317