Legionella pneumophila outer membrane vesicles: Isolation and analysis of their pro-inflammatory potential on macrophages

Journal of Visualized Experiments

Volumn 2017, Issue 120, 2017, Pages

  Jung, Anna Lena ^a   Hoffmann, Kerstin ^a   Herkt, Christina E ^a   Schulz, Christine ^a   Bertrams, Wilhelm ^a   Schmeck, Bernd ^a,b,c  

^a JUSTUS LIEBIG UNIVERSITY GIESSEN   (Germany)

^b German Center for Lung Research   (Germany)

^c UNIVERSITY HOSPITAL GIESSEN   (Germany)

Author keywords

Bacterial replication; Differential ultracentrifugation; Infection; Issue 120; Legionella pneumophila; Macrophage; Outer membrane vesicles; Pro inflammatory response; Purification

Indexed keywords

CELL LINE; CELL MEMBRANE; CELL VACUOLE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXOSOME; ISOLATION AND PURIFICATION; LEGIONELLA PNEUMOPHILA; MACROPHAGE; MACROPHAGE ACTIVATION; METABOLISM; MICROBIOLOGY;

CELL LINE; CELL MEMBRANE; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR VESICLES; LEGIONELLA PNEUMOPHILA; MACROPHAGE ACTIVATION; MACROPHAGES; VACUOLES;

EID: 85014385164     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/55146     Document Type: Article

References (33)

1. Cambronne, E. D., & Roy, C. R. Recognition and delivery of effector proteins into eukaryotic cells by bacterial secretion systems. Traffic. 7 (8), 929-939 (2006).
2. Ellis, T. N., & Kuehn, M. J. Virulence and immunomodulatory roles of bacterial outer membrane vesicles. Microbiol Mol Biol Rev. 74 (1), 81-94 (2010).
3. Beveridge, T. J. Structures of gram-negative cell walls and their derived membrane vesicles. J Bacteriol. 181 (16), 4725-4733 (1999).
4. Kuehn, M. J., & Kesty, N. C. Bacterial outer membrane vesicles and the host-pathogen interaction. Genes Dev. 19 (22), 2645-2655 (2005).
5. Chi, B., Qi, M., & Kuramitsu, H. K. Role of dentilisin in Treponema denticola epithelial cell layer penetration. Res Microbiol. 154 (9), 637-643 (2003).
6. Kolling, G. L., & Matthews, K. R. Export of virulence genes and Shiga toxin by membrane vesicles of Escherichia coli O157:H7. Appl Environ Microbiol. 65 (5), 1843-1848 (1999).
7. Horstman, A. L., & Kuehn, M. J. Enterotoxigenic Escherichia coli secretes active heat-labile enterotoxin via outer membrane vesicles. J Biol Chem. 275 (17), 12489-12496 (2000).
8. Dorward, D. W., Schwan, T. G., & Garon, C. F. Immune capture and detection of Borrelia burgdorferi antigens in urine, blood, or tissues from infected ticks, mice, dogs, and humans. J Clin Microbiol. 29 (6), 1162-1170 (1991).
9. Mashburn, L. M., & Whiteley, M. Membrane vesicles traffic signals and facilitate group activities in a prokaryote. Nature. 437 (7057), 422-425 (2005).
10. Kesty, N. C., Mason, K. M., Reedy, M., Miller, S. E., & Kuehn, M. J. Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. EMBO J. 23 (23), 4538-4549 (2004).
11. Abu Kwaik, Y., Gao, L. Y., Stone, B. J., Venkataraman, C., & Harb, O. S. Invasion of protozoa by Legionella pneumophila and its role in bacterial ecology and pathogenesis. Appl Environ Microbiol. 64 (9), 3127-3133 (1998).
12. Winn, W. C., Jr., & Myerowitz, R. L. The pathology of the Legionella pneumonias. A review of 74 cases and the literature. Hum Pathol. 12 (5), 401-422 (1981).
13. Ge, J., & Shao, F. Manipulation of host vesicular trafficking and innate immune defence by Legionella Dot/Icm effectors. Cell Microbiol. 13 (12), 1870-1880 (2011).
14. Hubber, A., & Roy, C. R. Modulation of host cell function by Legionella pneumophila type IV effectors. Annu Rev Cell Dev Biol. 26 261-283 (2010).
15. Fernandez-Moreira, E., Helbig, J. H., & Swanson, M. S. Membrane vesicles shed by Legionella pneumophila inhibit fusion of phagosomes with lysosomes. Infect Immun. 74 (6), 3285-3295 (2006).
16. Galka, F. et al. Proteomic characterization of the whole secretome of Legionella pneumophila and functional analysis of outer membrane vesicles. Infect Immun. 76 (5), 1825-1836 (2008).
17. Jager, J. et al. Human lung tissue explants reveal novel interactions during Legionella pneumophila infections. Infect Immun. 82 (1), 275-285 (2014).
18. Ellis, T. N., Leiman, S. A., & Kuehn, M. J. Naturally produced outer membrane vesicles from Pseudomonas aeruginosa elicit a potent innate immune response via combined sensing of both lipopolysaccharide and protein components. Infect Immun. 78 (9), 3822-3831 (2010).
19. Jager, J., Keese, S., Roessle, M., Steinert, M., & Schromm, A. B. Fusion of Legionella pneumophila outer membrane vesicles with eukaryotic membrane systems is a mechanism to deliver pathogen factors to host cell membranes. Cell Microbiol. (2014).
20. Jung, A. L. et al. Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages. PLoS Pathog. 12 (4), e1005592 (2016).
21. Jiang, Y., Kong, Q., Roland, K. L., & Curtiss, R., 3rd. Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. Int J Med Microbiol. 304 (3-4), 431-443 (2014).
22. Whitmire, W. M., & Garon, C. F. Specific and nonspecific responses of murine B cells to membrane blebs of Borrelia burgdorferi. Infect Immun. 61 (4), 1460-1467 (1993).
23. Ismail, S., Hampton, M. B., & Keenan, J. I. Helicobacter pylori outer membrane vesicles modulate proliferation and interleukin-8 production by gastric epithelial cells. Infect Immun. 71 (10), 5670-5675 (2003).
24. Jager, J., & Steinert, M. Enrichment of outer membrane vesicles shed by Legionella pneumophila. Methods Mol Biol. 954 225-230 (2013).
25. Park, E. K. et al. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 56 (1), 45-50 (2007).
26. Casson, C. N. et al. Human caspase-4 mediates noncanonical inflammasome activation against gram-negative bacterial pathogens. Proc Natl Acad Sci U S A. 112 (21), 6688-6693 (2015).
27. Molofsky, A. B., Shetron-Rama, L. M., & Swanson, M. S. Components of the Legionella pneumophila flagellar regulon contribute to multiple virulence traits, including lysosome avoidance and macrophage death. Infect Immun. 73 (9), 5720-5734 (2005).
28. Isaac, D. T., Laguna, R. K., Valtz, N., & Isberg, R. R. MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth. Proc Natl Acad Sci U S A. 112 (37), E5208-5217 (2015).
29. Zhu, W. et al. Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection. PLoS Pathog. 11 (3), e1004704 (2015).
30. Chutkan, H., Macdonald, I., Manning, A., & Kuehn, M. J. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods Mol Biol. 966 259-272 (2013).
31. Macdonald, I. A., & Kuehn, M. J. Stress-induced outer membrane vesicle production by Pseudomonas aeruginosa. J Bacteriol. 195 (13), 2971-2981 (2013).
32. Klimentova, J., & Stulik, J. Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria. Microbiol Res. 170 1-9 (2015).
33. https://www.novartis.com/news/media-releases/novartis-bexsero%C2%AE-vaccine-approved-fda-prevention-meningitis-b-leading-cause. (2016).