mRNA Expression and Amino Acid Transport Characteristics of Cultured Human Brain Microvascular Endothelial Cells (hBME)

Drug Metabolism and Pharmacokinetics

Volumn 17, Issue 4, 2002, Pages 367-373

  Umeki, Nobuo ^a   Fukasawa, Yoshiki ^b   Kohno, Yoshiro ^b   Ohtsuki, Sumio ^c   Hori, Satoko ^c   Watanabe, Yuki ^c   Terasaki, Tetsuya ^c  

^a KIRIN Pharmaceutical Res Laboratory   (Japan)

^b TAISHO PHARMACEUTICAL CO LTD   (Japan)

^c Grad Sch Pharmaceutical Sci   (Japan)

Author keywords

amino acid transporters; blood brain barrier; human brain microvascular endothelial cells (hBME); multidrug resistance protein 1 (MDR1); multidrug resistance associated protein 1 (MRP1)

Indexed keywords

EID: 85013697845     PISSN: 13474367     EISSN: 18800920     Source Type: Journal    
DOI: 10.2133/dmpk.17.367     Document Type: Article

References (27)

1. Hosoya, K., Tetsuka, K., Nagase, K., Tomi, M., Saeki, S., Ohtsuki, S., Takanaga, H., Yanai, N., Obinata, M., Kikuchi, A., Okano, T. and Terasaki, T.: Conditionally immortalized brain capillary endothelial cell lines established from a transgenic mouse harboring temperaturesensitive simian virus 40 large T-antigen gene. AAPS PharmSci., 2: E27 (2000).
2. Hosoya, K., Takashima, T., Tetsuka, K., Nagura, T., Ohtsuki, S., Takanaga, H., Ueda, M., Yanai, N., Obinata, M. and Terasaki, T.: mRNA expression and transport characterization of conditionally immortalized rat brain capillary endothelial cell lines; a new in vitro BBB model for drug targeting. J. Drug Target, 8: 357-370 (2000).
3. Tatsuta, T., Naito, M., Oh-hara, T., Sugawara, I. and Tsuruo, T.: Functional involvement of P-glycoprotein in blood-brain barrier. J. Biol. Chem., 267: 20383-20391 (1992).
4. Tsuji, A., Terasaki, T., Takabatake, Y., Tenda, Y., Tamai, I., Yamashima, T., Moritani, S., Tsuruo, T. and Yamashita, J.: P-Glycoprotein as the drug eOEux pump in primary cultured bovine brain capillary endothelial cells. Life Sci., 51: 1427-1437 (1992).
5. Golden, P. L. and Pardridge, W. M.: P-Glycoprotein on astrocyte foot processes of uňxed isolated human brain capillaries. Brain Res., 819: 143-146 (1999).
6. Pardridge, W. M., Golden, P. L., Kang, Y. S. and Bickel, U.: Brain microvascular and astrocyte localization of P-glycoprotein. J. Neurochem., 68: 1278-1285 (1997).
7. Tanigawara, Y., Okamura, N., Hirai, M., Yasuhara, M., Ueda, K., Kioka, N., Komano, T. and Hori, R.: Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLCPK1). J. Pharmacol. Exp. Ther., 263: 840-845 (1992)
8. Ueda, K., Okamura, N., Hirai, M., Tanigawara, Y., Saeki, T., Kioka, N., Komano, T. and Hori, R.: Human P-glycoprotein transports cortisol, aldosterone, and dexamethasone, but not progesterone. J. Biol. Chem., 267: 24248-24252 (1992).
9. Seetharaman, S., Barrand, M. A., Maskell, L. and Scheper, R. J.: Multidrug resistance-related transport proteins in isolated human brain microvessels and in cells cultured from these isolates. J. Neurochem., 70: 1151-1159 (1998).
10. Choi, T. B. and Pardridge, W. M.: Phenylalanine transport at the human blood-brain barrier. Studies with isolated human brain capillaries. J. Biol. Chem., 261: 6536-6541 (1986)
11. van Bree, J. B., Heijligers-Feijen, C. D., de Boer, A. G., Danhof, M. and Breimer, D. D.: Stereoselective transport of baclofen across the blood-brain barrier in rats as determined by the unit impulse response methodology. Pharm. Res., 8: 259-262 (1991).
12. Cornford, E. M., Young, D., Paxton, J. W., Finlay, G. J., Wilson, W. R. and Pardridge, W. M.: Melphalan penetration of the blood-brain barrier via the neutral amino acid transporter in tumor-bearing brain. Cancer Res., 52: 138-143 (1992).
13. Gomes, P. and Soares-da-Silva, P.: L-DOPA transport properties in an immortalised cell line of rat capillary cerebral endothelial cells, RBE 4. Brain Res., 829: 143-150 (1999).
14. Kanai, Y., Segawa, H., Miyamoto, K., Uchino, H., Takeda, E. and Endou, H.: Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem., 273: 23629-23632 (1998).
15. Boado, R. J., Li, J. Y., Nagaya, M., Zhang, C. and Pardridge, W. M.: Selective expression of the large neutral amino acid transporter at the blood-brain barrier. Proc. Natl. Acad. Sci. USA, 96: 12079-12084 (1999).
16. Yanagida, O., Kanai, Y., Chairoungdua, A., Kim, D. K., Segawa, H., Nii, T., Cha, S. H., Matsuo, H., Fukushima, J., Fukasawa, Y., Tani, Y., Taketani, Y., Uchino, H., Kim, J. Y., Inatomi, J., Okayasu, I., Miyamoto, K., Takeda, E., Goya, T. and Endou, H.: Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. Biochim. Biophys. Acta, 1514: 291-302 (2001).
17. Hosoya, K., Sugawara, M., Asaba, H. and Terasaki, T.: Blood-brain barrier produces signîcant eOEux of Laspartic acid but not D-aspartic acid: in vivo evidence using the brain eOEux index method. J. Neurochem., 73: 1206-1211 (1999).
18. Hutchison, H. T., Eisenberg, H. M. and Haber, B.: High-ânity transport of glutamate in rat brain microvessels. Exp. Neurol., 87: 260-269 (1985).
19. O'Kane, R. L., Martinez-Lopez, I., De Joseph, M. R., Vina, J. R. and Hawkins, R. A.: Na+-dependent glutamate transporters (EAAT1, EAAT2, and EAAT3) of the blood-brain barrier. A mechanism for glutamate removal. J. Biol. Chem., 274: 31891-31895 (1999).
20. Arriza, J. L., Fairman, W. A., Wadiche, J. I., Murdoch, G. H., Kavanaugh, M. P. and Amara, S. G.: Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex. J. Neurosci., 14: 5559-5569 (1994).
21. Smith, Q. R. and Stoll, J.: Blood-brain barrier amino acid transport. In Pardridge, W. M. (ed.): Introduction to blood-brain barrier: methodology, biology and pathology, Cambridge, Cambridge University Press, 1998, pp. 188-197.
22. Wang, H., Kavanaugh, M. P., North, R. A. and Kabat, D.: Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature, 352: 729-731 (1991).
23. Cornford, E. M., Hyman, S. and Swartz, B. E.: The human brain GLUT1 glucose transporter: ultrastructural localization to the blood-brain barrier endothelia. J. Cereb. Blood Flow Metab., 14: 106-112 (1994).
24. Gerhart, D. Z., Enerson, B. E., Zhdankina, O. Y., Leino, R. L. and Drewes, L. R.: Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats. Am. J. Physiol., 273: E207-E213 (1997).
25. Sugawara, I., Hamada, H., Tsuruo, T. and Mori, S.: Specialized localization of P-glycoprotein recognized by MRK 16 monoclonal antibody in endothelial cells of the brain and the spinal cord. J. Cancer Res., 81: 727-730 (1990).
26. Zhang, Y., Han, H., Elmquist, W. F. and Miller, D.W.: Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells. Brain Res., 876: 148-153 (2000).
27. Huai-Yun, H., Secrest, D. T., Mark, K. S., Carney, D., Brandquist, C., Elmquist, W. F. and Miller, D. W.: Expression of multidrug resistance-associated protein (MRP) in brain microvessel endothelial cells. Biochem. Biophys. Res. Commun., 243: 816-820 (1998).