A cocktail of three virulent bacteriophages prevents Vibrio cholerae infection in animal models

Nature Communications

Volumn 8, Issue , 2017, Pages

  Yen, Minmin ^a,b   Cairns, Lynne S ^a   Camilli, Andrew ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b TUFTS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL PROTEIN;

ANIMAL; BACTERIOPHAGE; BACTERIUM; CHOLERA; COLONIZATION; DEHYDRATION; DIARRHEAL DISEASE; LAGOMORPH; MUTATION; PATHOGEN; RODENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC PROPHYLAXIS; ARTICLE; BACTERIAL CELL; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL SURVIVAL; BACTERIAL VIRULENCE; BACTERICIDAL ACTIVITY; CHOLERA; CONTROLLED STUDY; GENE MUTATION; GENETIC VARIABILITY; GENOME ANALYSIS; IN VITRO STUDY; IN VIVO STUDY; INFANT; INFANT RABBIT; INFECTION PREVENTION; MOUSE; NONHUMAN; SMALL INTESTINE; VIBRIO CHOLERAE; VIBRIOPHAGE; VIRUS VIRULENCE; WHOLE GENOME SEQUENCING; ANIMAL; BACTERIAL LOAD; BACTERIOPHAGE; DISEASE MODEL; GASTROINTESTINAL TRACT; GENETICS; HUMAN; LEPORIDAE; METABOLISM; MICROBIOLOGY; MUTATION; PATHOGENICITY; PHYSIOLOGY; VIROLOGY; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); ORYCTOLAGUS CUNICULUS; VIBRIO CHOLERAE;

ANIMALS; BACTERIAL LOAD; BACTERIAL PROTEINS; BACTERIOPHAGES; CHOLERA; DISEASE MODELS, ANIMAL; GASTROINTESTINAL TRACT; HUMANS; MICE; MODELS, ANIMAL; MUTATION; RABBITS; VIBRIO CHOLERAE; VIRULENCE;

EID: 85011623848     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms14187     Document Type: Article

References (25)

1. Zuckerman, J. N., Rombo, L. & Fisch, A. The true burden and risk of cholera: implications for prevention and control. Lancet Infect. Dis. 7, 521-530 (2007).
2. Luquero, F. J. et al. Mortality rates during cholera epidemic, Haiti, 2010-2011. Emerg. Infect. Dis. 22, 410-416 (2016).
3. Bagcchi, S. Cholera in Iraq strains the fragile state. Lancet Infect. Dis. 16, 24-25 (2016).
4. Qadri, F. et al. Efficacy of a single-dose, inactivated oral cholera vaccine in Bangladesh. N. Engl. J. Med. 374, 1723-1732 (2016).
5. Taylor, D. L., Kahawita, T. M., Cairncross, S. & Ensink, J. H. The impact of water, sanitation and hygiene interventions to control cholera: A systematic review. PLoS ONE 10, e0135676 (2015).
6. Harris, J. B. et al. Susceptibility to Vibrio cholerae infection in a cohort of household contacts of patients with cholera in Bangladesh. PLoS Negl. Trop. Dis. 2, e221 (2008).
7. Reveiz, L. et al. Chemoprophylaxis in contacts of patients with cholera: systematic review and meta-Analysis. PLoS ONE 6, e27060 (2011).
8. Wittebole, X., De Roock, S. & Opal, S. M. A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens. Virulence 5, 226-235 (2014).
9. Dutta, N. K. & Panse, M. V. An experimental study on the usefulness of bacteriophage in the prophylaxis and treatment of cholera. Bull. World Health Organ. 28, 357-360 (1963).
10. Jaiswal, A., Koley, H., Ghosh, A., Palit, A. & Sarkar, B. Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model. Microbes Infect. 15, 152-156 (2013).
11. Jaiswal, A., Koley, H., Mitra, S., Saha, D. R. & Sarkar, B. Comparative analysis of different oral approaches to treat Vibrio cholerae infection in adult mice. Int. J. Med. Microbiol. 304, 422-430 (2014).
12. Seed, K. D. et al. Evidence of a dominant lineage of Vibrio cholerae-specific lytic bacteriophages shed by cholera patients over a 10-year period in Dhaka, Bangladesh. mBio 2, e00334-00410 (2011).
13. Seed, K. D. et al. Phase variable O antigen biosynthetic genes control expression of the major protective antigen and bacteriophage receptor in Vibrio cholerae O1. PLoS Pathog. 8, e1002917 (2012).
14. Seed, K. D. et al. Evolutionary consequences of intra-patient phage predation on microbial populations. eLife 3, e03497 (2014).
15. Heidelberg, J. F. et al. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406, 477-484 (2000).
16. Lazinski, D. W. & Camilli, A. Homopolymer tail-mediated ligation PCR: A streamlined and highly efficient method for DNA cloning and library construction. BioTechniques 54, 25-34 (2013).
17. Heidelberg, J. F. et al. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406, 477-483 (2000).
18. Chatterjee, S. N. & Chaudhuri, K. Lipopolysaccharides of Vibrio cholerae II. Genetics of biosynthesis. Biochim. Biophys. Acta 1690, 93-109 (2004).
19. Kamp, H. D., Patimalla-Dipali, B., Lazinski, D. W., Wallace-Gadsden, F. & Camilli, A. Gene fitness landscapes of Vibrio cholerae at important stages of its life cycle. PLoS Pathog. 9, e1003800 (2013).
20. Pritchard, J. R. et al. ARTIST: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing. PLoS Genet. 10, e1004782 (2014).
21. Fu, Y., Waldor, M. K. & Mekalanos, J. J. Tn-Seq analysis of Vibrio cholerae intestinal colonization reveals a role for T6SS-mediated antibacterial activity in the host. Cell Host Microbe 14, 652-663 (2013).
22. Abel, S. & Waldor, M. K. Infant rabbit model for diarrheal diseases. Curr. Protoc. Microbiol. 38 6A 6 1-15 (2015).
23. Levine, M. M. et al. The pathogenicity of nonenterotoxigenic Vibrio cholerae Serogroup 01 Biotype EI Tor isolated from sewage water in Brazil. J. Infect. Dis. 145, 296-299 (1982).
24. Schild, S., Nelson, E. J. & Camilli, A. Immunization with Vibrio cholerae outer membrane vesicles induces protective immunity in mice. Infect. Immun. 76, 4554-4563 (2008).
25. Seed, K. D., Lazinski, D. W., Calderwood, S. B. & Camilli, A. A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity. Nature 494, 489-491 (2013).