Development and characterization of anti-glycopeptide monoclonal antibodies against human podoplanin, using glycan-deficient cell lines generated by CRISPR/Cas9 and TALEN

Cancer Medicine

Volumn 6, Issue 2, 2017, Pages 382-396

  Kaneko, Mika K ^a   Nakamura, Takuro ^a   Honma, Ryusuke ^a,b   Ogasawara, Satoshi ^a   Fujii, Yuki ^a   Abe, Shinji ^c   Takagi, Michiaki ^b   Harada, Hiroyuki ^d   Suzuki, Hiroyoshi ^e   Nishioka, Yasuhiko ^c   Kato, Yukinari ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b YAMAGATA UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKUSHIMA   (Japan)

^d TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^e SENDAI CITY MEDICAL CENTER   (Japan)

Author keywords

Epitope; glycopeptide; monoclonal antibody; podoplanin; sialic acid

Indexed keywords

ARGININE; EPITOPE; GLYCAN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY LPMAB 21; PODOPLANIN; SIALIC ACID; THREONINE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; MEMBRANE PROTEIN; PDPN PROTEIN, HUMAN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY DETECTION; ARTICLE; CANCER CELL LINE; CONTROLLED STUDY; CRISPR CAS SYSTEM; EMBRYO; EPITHELIUM CELL; FEMALE; GLIOBLASTOMA CELL LINE; HEK293 CELL LINE; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG ALVEOLUS CELL TYPE 1; MOUSE; NONHUMAN; PROTEIN EXPRESSION; SIALYLATION; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; CHO CELL LINE; CRICETULUS; GENE INACTIVATION; GENETICS; IMMUNOLOGY; METABOLISM; TUMOR CELL LINE;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE, TUMOR; CHO CELLS; CRICETULUS; CRISPR-CAS SYSTEMS; EPITOPES; GENE KNOCKOUT TECHNIQUES; HEK293 CELLS; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; ORGAN SPECIFICITY;

EID: 85010380029     PISSN: None     EISSN: 20457634     Source Type: Journal    
DOI: 10.1002/cam4.954     Document Type: Article

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