Optimisation of methods for bacterial skin microbiome investigation: Primer selection and comparison of the 454 versus MiSeq platform

BMC Microbiology

Volumn 17, Issue 1, 2017, Pages

  Castelino, Madhura ^a   Eyre, Stephen ^b   Moat, John ^b   Fox, Graeme ^c   Martin, Paul ^b   Ho, Pauline ^a   Upton, Mathew ^d   Barton, Anne ^a,b  

^a MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

^d PLYMOUTH UNIVERSITY PENINSULA SCHOOLS OF MEDICINE AND DENTISTRY   (United Kingdom)

Author keywords

16s rRNA gene; Bacterial microbiome; Low biomass; Methods; Miseq platform; Next generation sequencing; Optimisation; Primer selection; Skin microbiome

Indexed keywords

DNA; RIBOSOME RNA; RNA 16S; BACTERIAL DNA; PRIMER DNA;

ARTICLE; ATOPIC DERMATITIS; BACTERIAL MICROBIOME; BACTERIAL STRAIN; CHIMERA; CLINICAL PROTOCOL; CONTROLLED STUDY; CORYNEBACTERIUM; COST EFFECTIVENESS ANALYSIS; DEINOCOCCUS; ENHYDROBACTER; GENE SEQUENCE; HUMAN; MAJOR CLINICAL STUDY; MICROCOCCUS; PARACOCCUS; PHYLUM; PROCESS OPTIMIZATION; PROPIONIBACTERIUM; PSORIASIS; SKIN FLORA; STAPHYLOCOCCUS; ADULT; BACTERIAL GENE; BACTERIUM; BACTERIUM IDENTIFICATION; BIOLOGY; BIOMASS; CLASSIFICATION; DEVICES; DNA CONTAMINATION; DNA SEQUENCE; GENETIC VARIATION; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROFLORA; MIDDLE AGED; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PROCEDURES; SKIN;

ADULT; BACTERIA; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; BIOMASS; COMPUTATIONAL BIOLOGY; DNA CONTAMINATION; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; GENETIC VARIATION; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MICROBIOTA; MIDDLE AGED; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SKIN;

EID: 85010016477     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-017-0927-4     Document Type: Article

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