Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine

BMC Genomics

Volumn 18, Issue 1, 2017, Pages

  Chen, Gary G ^a   Gross, Jeffrey A ^a   Lutz, Pierre Eric ^a   Vaillancourt, Kathryn ^a   Maussion, Gilles ^a   Bramoulle, Alexandre ^a   Théroux, Jean François ^a   Gardini, Elena S ^b   Ehlert, Ulrike ^b   Bourret, Geneviève ^c   Masurel, Aurélie ^c   Lepage, Pierre ^c   Mechawar, Naguib ^a   Turecki, Gustavo ^a   Ernst, Carl ^a  

^a MCGILL UNIVERSITY   (Canada)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

5 hydroxymethylcytosine; 5 methylcytosine; Customized MiSeq sequencing; Multiplexed barcoding of target amplicon libraries; Multiplexed PCR directed BS amplicons; Next generation sequencing; Targeted bisulfite sequencing

Indexed keywords

5 HYDROXYMETHYLCYTOSINE; 5 METHYLCYTOSINE; GENOMIC DNA; 5-HYDROXYMETHYLCYTOSINE; HYDROGEN SULFITE; SULFITE;

ADULT; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; EPIGENETICS; GENE SEQUENCE; GENOME ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; LIMIT OF QUANTITATION; MALE; MEASUREMENT ACCURACY; MULTIPLEX POLYMERASE CHAIN REACTION; NORMAL HUMAN; VALIDATION PROCESS; ANALOGS AND DERIVATIVES; DNA METHYLATION; DNA SEQUENCE; DRUG EFFECTS; METABOLISM; OXIDATION REDUCTION REACTION; PROCEDURES;

5-METHYLCYTOSINE; ADULT; DNA METHYLATION; HUMANS; MALE; OXIDATION-REDUCTION; SEQUENCE ANALYSIS, DNA; SULFITES;

EID: 85009508342     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/s12864-017-3489-9     Document Type: Article

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