A pilot study to compare the detection of HPV-16 biomarkers in salivary oral rinses with tumour p16INK4a expression in head and neck squamous cell carcinoma patients

BMC Cancer

Volumn 16, Issue 1, 2016, Pages

  Chai, Ryan C ^a,b   Lim, Yenkai ^c   Frazer, Ian H ^a   Wan, Yunxia ^c   Perry, Christopher ^d   Jones, Lee ^c   Lambie, Duncan ^e   Punyadeera, Chamindie ^c  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b GARVAN INSTITUTE OF MEDICAL RESEARCH   (Australia)

^c QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^d PRINCESS ALEXANDRA HOSPITAL   (Australia)

^e IQ PATHOLOGY   (Australia)

Author keywords

Early detection; HNSCC; HPV; OPSCC; Saliva

Indexed keywords

BIOLOGICAL MARKER; CYCLIN DEPENDENT KINASE INHIBITOR 2A; MOUTHWASH; VIRUS DNA; VIRUS RNA; TUMOR MARKER;

ADULT; ARTICLE; CERVICAL LYMPH NODE; CONTROLLED STUDY; DNA DETERMINATION; FEASIBILITY STUDY; HEAD AND NECK SQUAMOUS CELL CARCINOMA; HUMAN; HUMAN PAPILLOMAVIRUS TYPE 16; HUMAN TISSUE; HYPOPHARYNX CARCINOMA; INTERMETHOD COMPARISON; LARYNX CARCINOMA; MAJOR CLINICAL STUDY; MOUTH SQUAMOUS CELL CARCINOMA; NASOPHARYNX CARCINOMA; NONHUMAN; OROPHARYNX CARCINOMA; PHARYNX CARCINOMA; PILOT STUDY; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SALIVA ANALYSIS; SALIVARY GLAND CARCINOMA; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; AGED; CARCINOMA, SQUAMOUS CELL; COMPLICATION; FEMALE; GENETICS; HEAD AND NECK NEOPLASMS; MALE; MIDDLE AGED; PAPILLOMAVIRUS INFECTION; SALIVA; VERY ELDERLY; VIROLOGY; VIRUS GENE;

ADULT; AGED; AGED, 80 AND OVER; BIOMARKERS, TUMOR; CARCINOMA, SQUAMOUS CELL; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DNA, VIRAL; FEMALE; GENES, VIRAL; HEAD AND NECK NEOPLASMS; HUMAN PAPILLOMAVIRUS 16; HUMANS; MALE; MIDDLE AGED; PAPILLOMAVIRUS INFECTIONS; POLYMERASE CHAIN REACTION; SALIVA;

EID: 85007508305     PISSN: None     EISSN: 14712407     Source Type: Journal    
DOI: 10.1186/s12885-016-2217-1     Document Type: Article

References (31)

1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69-90.
2. Sturgis EM, Cinciripini PM. Trends in head and neck cancer incidence in relation to smoking prevalence. Cancer. 2007;110(7):1429-35.
3. Hashibe M, Brennan P, Benhamou S, Castellsague X, Chen C, Curado MP, et al. Alcohol drinking in never users of tobacco, cigarette smoking in never drinkers, and the risk of head and neck cancer: pooled analysis in the international head and neck cancer epidemiology consortium. J Natl Cancer Inst. 2007;99(10):777-89.
4. Marur S, D'Souza G, Westra WH, Forastiere AA. HPV-associated head and neck cancer: a virus-related cancer epidemic. Lancet Oncol. 2010;11(8):781-9.
5. Chai RC, Lambie D, Verma M, Punyadeera C. Current trends in the etiology and diagnosis of HPV-related head and neck cancers. Cancer Medicine. 2015;4(4):596-607.
6. Zhang HS, Postigo AA, Dean DC. Active transcriptional repression by the Rb-E2F complex mediates G1 arrest triggered by p16INK4a, TGFβ, and contact inhibition. Cell. 1999;97(1):53-61.
7. Lima DP, Diniz DG, Moimaz SAS, Sumida DH, Okamoto AC. Saliva: reflection of the body. Int J Infect Dis. 2010;14(3):e184-8.
8. Pfaffe T, Cooper-White J, Beyerlein P, Kostner K, Punyadeera C. Diagnostic potential of saliva: current state and future applications. Clin Chem. 2011; 57(5):675-87.
9. Zhang X, Wan Y, Cooper-White J, Dimeski G, Atherton J, Punyadeera C. Quantification of D-dimer levels in human saliva. Bioanalysis. 2013;5(18):2249-56.
10. Zhang X, Kulasinghe A, Karim R, Punyadeera C. Saliva diagnostics for oral diseases. In: Streckfus CF, editor. Advances in salivary diagnostics. Berlin, Germany: Springer Berlin Heidelberg; 2015. p. 131-56.
11. Salazar C, Calvopiña D, Punyadeera C. miRNAs in human papilloma virus associated oral and oropharyngeal squamous cell carcinomas. Expert Rev Mol Diagn. 2014;14(8):1033-40.
12. Pandit P, Cooper-White J, Punyadeera C. High-yield RNA-extraction method for saliva. Clin Chem. 2013;59(7):1118-22.
13. Soler C, Chardonnet Y, Allibert P, Euvrard S, Mandrand B, Thivolet J. Detection of multiple types of human papillomavirus in a giant condyloma from a grafted patient. Analysis by immunohistochemistry, in situ hybridisation, southern blot and polymerase chain reaction. Virus Res. 1992;23(3):193-208.
14. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primerdirected enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239(4839):487-91.
15. Walboomers JMM, Jacobs MV, Manos MM, Bosch FX, Kummer JA, Shah KV, et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol. 1999;189(1):12-9.
16. Ha PK, Pai SI, Westra WH, Gillison ML, Tong BC, Sidransky D, et al. Real-time quantitative PCR demonstrates Low prevalence of human papillomavirus type 16 in premalignant and malignant lesions of the oral cavity. Clin Cancer Res. 2002;8(5):1203-9.
17. Team RC: R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2012. In.: ISBN 3-900051-07-0; 2014. http://www.gbif.org/resource/81287.
18. Ang KK, Harris J, Wheeler R, Weber R, Rosenthal DI, Nguyen-Tân PF, et al. Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010;363(1):24-35.
19. Walvekar RR, Li RJ, Gooding WE, Gibson MK, Heron D, Johnson JT, et al. Role of surgery in limited (T1-2, N0-1) cancers of the oropharynx. Laryngoscope. 2008;118(12):2129-34.
20. Lewis Jr J. p16 immunohistochemistry as a standalone test for risk stratification in oropharyngeal squamous cell carcinoma. Head Neck Pathol. 2012;6(1):75-82.
21. Koskinen WJ, Chen RW, Leivo I, Mäkitie A, Bäck L, Kontio R, et al. Prevalence and physical status of human papillomavirus in squamous cell carcinomas of the head and neck. Int J Cancer. 2003;107(3):401-6.
22. Zhao M, Rosenbaum E, Carvalho AL, Koch W, Jiang W, Sidransky D, et al. Feasibility of quantitative PCR-based saliva rinse screening of HPV for head and neck cancer. Int J Cancer. 2005;117(4):605-10.
23. Chen C-M, Shyu M-P, Au L-C, Chu H-W, Cheng WTK, Choo K-B. Analysis of deletion of the integrated human papillomavirus 16 sequence in cervical cancer: a rapid multiplex polymerase chain reaction approach. J Med Virol. 1994;44(2):206-11.
24. Ahn SM, Chan JK, Zhang Z, et al. Saliva and plasma quantitative polymerase chain reaction-based detection and surveillance of human papillomavirus-related head and neck cancer. JAMA Otolaryngology-Head & Neck Surgery. 2014;140(9):846-54.
25. Agrawal Y, Koch WM, Xiao W, Westra WH, Trivett AL, Symer DE, et al. Oral human papillomavirus infection before and after treatment for human papillomavirus 16-positive and human papillomavirus 16-negative head and neck squamous cell carcinoma. Clin Cancer Res. 2008;14(21):7143-50.
26. Smeets SJ, Hesselink AT, Speel E-JM, Haesevoets A, Snijders PJF, Pawlita M, et al. A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen. Int J Cancer. 2007;121(11):2465-72.
27. Deng Z, Hasegawa M, Kiyuna A, Matayoshi S, Uehara T, Agena S, et al. Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma. Head Neck. 2013;35(6):800-8.
28. Holzinger D, Schmitt M, DyckhoffG, Benner A, Pawlita M, Bosch FX. Viral RNA patterns and high viral load reliably define oropharynx carcinomas with active HPV16 involvement. Cancer Res. 2012;72(19):4993-5003.
29. Park NJ, Li Y, Yu T, Brinkman BMN, Wong DT. Characterization of RNA in saliva. Clin Chem. 2006;52(6):988-94.
30. Venuti A, Paolini F. HPV detection methods in head and neck cancer. Head Neck Pathol. 2012;6(1):63-74.
31. Kornegay JR, Roger M, Davies PO, Shepard AP, Guerrero NA, Lloveras B, et al. International proficiency study of a consensus L1 PCR assay for the detection and typing of human papillomavirus DNA: evaluation of accuracy and intralaboratory and interlaboratory agreement. J Clin Microbiol. 2003;41(3):1080-6.