High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Wevers, Nienke R ^a,b   Van Vught, Remko ^a   Wilschut, Karlijn J ^a   Nicolas, Arnaud ^a,c   Chiang, Chiwan ^a   Lanz, Henriette L ^a   Trietsch, Sebastiaan J ^a   Joore, Jos ^a   Vulto, Paul ^a,c  

^a MIMETAS BV   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; LAMININ; MATRIGEL; NEUROTOXIN; PROTEOGLYCAN;

ACTION POTENTIAL; CELL CULTURE TECHNIQUE; CELL SHAPE; COCULTURE; CYTOLOGY; DEVICES; DRUG COMBINATION; DRUG EFFECT; GLIA; HIGH THROUGHPUT SCREENING; HUMAN; INDUCED PLURIPOTENT STEM CELL; LAB ON A CHIP; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEURAL STEM CELL; NEURITE; ORGANOID; PROCEDURES; ULTRASTRUCTURE;

ACTION POTENTIALS; CELL CULTURE TECHNIQUES; CELL SHAPE; COCULTURE TECHNIQUES; COLLAGEN; DRUG COMBINATIONS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LAB-ON-A-CHIP DEVICES; LAMININ; NEURAL STEM CELLS; NEURITES; NEUROGENESIS; NEUROGLIA; NEURONS; NEUROTOXINS; ORGANOIDS; PROTEOGLYCANS;

EID: 85006085663     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep38856     Document Type: Article

References (36)

1. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-76 (2006).
2. Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-72 (2007).
3. Hu, B.-Y. & Zhang, S.-C. Differentiation of spinal motor neurons from pluripotent human stem cells. Nat. Protoc. 4, 1295-304 (2009).
4. Swistowski, A. et al. Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. Stem Cells 28, 1893-904 (2010).
5. Shi, Y., Kirwan, P. & Livesey, F. J. Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat. Protoc. 7, 1836-46 (2012).
6. Yu, D. X. et al. Modeling hippocampal neurogenesis using human pluripotent stem cells. Stem cell reports 2, 295-310 (2014).
7. Erceg, S., Lukovic, D., Moreno-Manzano, V., Stojkovic, M. & Bhattacharya, S. S. Derivation of cerebellar neurons from human pluripotent stem cells. Curr. Protoc. Stem Cell Biol. Chapter 1, Unit 1H.5 (2012).
8. Krencik, R. & Zhang, S.-C. Directed differentiation of functional astroglial subtypes from human pluripotent stem cells. Nat. Protoc. 6, 1710-7 (2011).
9. Wang, S. et al. Human iPSC-derived oligodendrocyte progenitor cells can myelinate and rescue a mouse model of congenital hypomyelination. Cell Stem Cell 12, 252-64 (2013).
10. Ohgidani, M. et al. Direct induction of ramified microglia-like cells from human monocytes: dynamic microglial dysfunction in Nasu-Hakola disease. Sci. Rep. 4, 4957 (2014).
11. Lippmann, E. S. et al. Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat. Biotechnol. 30, 783-91 (2012).
12. Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262-5 (2009).
13. Huch, M. et al. Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell 160, 299-312 (2015).
14. Takasato, M. et al. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature 526, 564-8 (2015).
15. Lancaster, M. A. et al. Cerebral organoids model human brain development and microcephaly. Nature 501, 373-9 (2013).
16. Garcez, P. P. et al. Zika virus impairs growth in human neurospheres and brain organoids. Science 352, 816-8 (2016).
17. Trietsch, S. J., Israëls, G. D., Joore, J., Hankemeier, T. & Vulto, P. Microfluidic titer plate for stratified 3D cell culture. Lab Chip 13, 3548-54 (2013).
18. Moreno, E. L. et al. Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture. Lab Chip 15, 2419-28 (2015).
19. Jang, M., Neuzil, P., Volk, T., Manz, A. & Kleber, A. On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro. Biomicrofluidics 9, 34113 (2015).
20. Vulto, P. et al. Phaseguides: a paradigm shift in microfluidic priming and emptying. Lab Chip 11, 1596-602 (2011).
21. Owens, D. F., Boyce, L. H., Davis, M. B. & Kriegstein, A. R. Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging. J. Neurosci. 16, 6414-23 (1996).
22. Leinekugel, X. et al. GABA is the principal fast-acting excitatory transmitter in the neonatal brain. Adv. Neurol. 79, 189-201 (1999).
23. Kiernan, M. C., Isbister, G. K., Lin, C. S.-Y., Burke, D. & Bostock, H. Acute tetrodotoxin-induced neurotoxicity after ingestion of puffer fish. Ann. Neurol. 57, 339-48 (2005).
24. Yee, S. & Choi, B. H. Oxidative stress in neurotoxic effects of methylmercury poisoning. Neurotoxicology 17, 17-26 (1996).
25. Gant, D. B., Eldefrawi, M. E. & Eldefrawi, A. T. Cyclodiene insecticides inhibit GABAA receptor-regulated chloride transport. Toxicol. Appl. Pharmacol. 88, 313-21 (1987).
26. DeCaprio, A. P., Briggs, R. G., Jackowski, S. J. & Kim, J. C. Comparative neurotoxicity and pyrrole-forming potential of 2,5-hexanedione and perdeuterio-2,5-hexanedione in the rat. Toxicol. Appl. Pharmacol. 92, 75-85 (1988).
27. Hoffman, R. M. To do tissue culture in two or three dimensions? that is the question. Stem Cells 11, 105-111 (1993).
28. Cukierman, E., Pankov, R., Stevens, D. R. & Yamada, K. M. Taking cell-matrix adhesions to the third dimension. Science 294, 1708-12 (2001).
29. Pampaloni, F., Reynaud, E. G. & Stelzer, E. H. K. The third dimension bridges the gap between cell culture and live tissue. Nat. Rev. Mol. Cell Biol. 8, 839-45 (2007).
30. Ravi, M., Paramesh, V., Kaviya, S. R., Anuradha, E. & Solomon, F. D. P. 3D cell culture systems: advantages and applications. J. Cell. Physiol. 230, 16-26 (2015).
31. Peretz, H., Talpalar, A. E., Vago, R. & Baranes, D. Superior Survival and Durability of Neurons and Astrocytes on 3-Dimensional Aragonite Biomatrices. Tissue Eng. 13, 461-472 (2007).
32. Zare-Mehrjardi, N. et al. Differentiation of embryonic stem cells into neural cells on 3D poly (D, L-lactic acid) scaffolds versus 2D cultures. Int. J. Artif. Organs 34, 1012-23 (2011).
33. Kelava, I. & Lancaster, M. A. Dishing out mini-brains: Current progress and future prospects in brain organoid research. Dev. Biol., 10.1016/j.ydbio.2016.06.037 (2016).
34. Wevers, N. R. & de Vries, H. E. Morphogens and blood-brain barrier function in health and disease. Tissue barriers 4, 10.1080/21688370.2015.1090524 (2016).
35. Schork, N. J. Personalized medicine: Time for one-person trials. Nature 520, 609-11 (2015).
36. Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676-82 (2012).