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Taking a computational modelling approach, the authors tested parameters to explain empirical data on RNA accumulation and transport in the phloem. They found that RNA abundance and half-life are sufficient to explain almost all of the observed data, and did not find any structural or sequence-specific motifs, which indicates that trafficking of RNA into the phloem is not selective. Arguably, this model has been supported by a yet more recent study showing that transcripts 3'UTR tRNA-like structures, which enhance mRNA stability and thus increase RNA abundance and half-life, are more abundant in the phloem than transcripts without that stabilizing structure.
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The authors use genetics and biochemistry to show that long-distance transport of signals involved in systemic acquired resistance (SAR) begins with several signals, including azelaic acid and glycerol-3-phosphate, moving from infected cells to the plant vasculature through PD. The study relies primarily on modulating expression of PDLP5, a PD-localized protein that is transcriptionally induced by the Pseudomonas effector, HopW1-1, and testing its impacts on SAR response and SAR-associated chemical accumulation. Unexpectedly, however, knockout pdlp5 mutants and overexpression 35S:PDLP5 lines, which are believed to increase and decrease PD transport, respectively, are both defective in SAR, suggesting that PDLP5 may have functions independent of its regulation of PD, or that precise spatiotemporal regulation of PD is required to initiate and spread the SAR signals.
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60• Lim, G.H., Shine, M.B., De Lorenzo, L., Yu, K., Cui, W., Navarre, D., Hunt, A.G., Lee, J.Y., Kachroo, A., Kachroo, P., plasmodesmata localizing proteins regulate transport and signaling during systemic acquired immunity in plants. Cell Host Microbe 19 (2016), 541–549 The authors use genetics and biochemistry to show that long-distance transport of signals involved in systemic acquired resistance (SAR) begins with several signals, including azelaic acid and glycerol-3-phosphate, moving from infected cells to the plant vasculature through PD. The study relies primarily on modulating expression of PDLP5, a PD-localized protein that is transcriptionally induced by the Pseudomonas effector, HopW1-1, and testing its impacts on SAR response and SAR-associated chemical accumulation. Unexpectedly, however, knockout pdlp5 mutants and overexpression 35S:PDLP5 lines, which are believed to increase and decrease PD transport, respectively, are both defective in SAR, suggesting that PDLP5 may have functions independent of its regulation of PD, or that precise spatiotemporal regulation of PD is required to initiate and spread the SAR signals.
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