Opening the channel: The two functional interfaces of pseudomonas aeruginosa OpmH with the triclosan efflux pump TriABC

Journal of Bacteriology

Volumn 198, Issue 23, 2016, Pages 3176-3185

  Ntreh, Abigail T ^a   Weeks, J W ^a   Nickels, Logan M ^a   Zgurskaya, Helen I ^a  

^a UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER A1; MEMBRANE FUSION PROTEIN; OUTER MEMBRANE PROTEIN; TRICLOSAN; ANTIINFECTIVE AGENT; CARRIER PROTEIN; OPMH PROTEIN, PSEUDOMONAS AERUGINOSA;

ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN STRUCTURE; PSEUDOMONAS AERUGINOSA; GENETICS; METABOLISM; TRANSPORT AT THE CELLULAR LEVEL;

ANTI-BACTERIAL AGENTS; BIOLOGICAL TRANSPORT; MEMBRANE TRANSPORT PROTEINS; PSEUDOMONAS AERUGINOSA; TRICLOSAN;

EID: 84997076988     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00535-16     Document Type: Article

References (27)

1. Nikaido H, Zgurskaya HI. 1999. Antibiotic efflux mechanisms. Curr Opin Infect Dis 12:529-536. http://dx.doi.org/10.1097/00001432-199912000-00001.
2. Krishnamoorthy G, Tikhonova EB, Dhamdhere G, Zgurskaya HI. 2013. On the role of TolC in multidrug efflux: the function and assembly of AcrAB-TolC tolerate significant depletion of intracellular TolC protein. Mol Microbiol 87:982-997. http://dx.doi.org/10.1111/mmi.12143.
3. Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C. 2000. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914-919. http://dx.doi.org/10.1038/35016007.
4. Andersen C, Koronakis E, Bokma E, Eswaran J, Humphreys D, Hughes C, Koronakis V. 2002. Transition to the open state of the TolC periplasmic tunnel entrance. Proc Natl Acad Sci U S A 99:11103-11108. http://dx.doi.org/10.1073/pnas.162039399.
5. Bavro VN, Pietras Z, Furnham N, Perez-Cano L, Fernandez-Recio J, Pei XY, Misra R, Luisi B. 2008. Assembly and channel opening in a bacterial drug efflux machine. Mol Cell 30:114-121. http://dx.doi.Org/10.1016/j.molcel.2008.02.015.
6. Zgurskaya HI, Weeks JW, Ntreh AT, Nickels LM, Wolloscheck D. 2015. Mechanism of coupling drug transport reactions located in two different membranes. Front Microbiol 6:100. http://dx.doi.org/10.3389/fmicb.2015.00100.
7. Jeong H, Kim JS, Song S, Shigematsu H, Yokoyama T, Hyun J, Ha NC. 2016. Pseudoatomic structure of the tripartite multidrug efflux pump AcrAB-TolC reveals the intermeshing cogwheel-like interaction between AcrA and TolC. Structure 24:272-276. http://dx.doi.org/10.1016/j.str.2015.12.007.
8. Du D, Wang Z, James NR, Voss JE, Klimont E, Ohene-Agyei T, Venter H, Chiu W, Luisi BF. 2014. Structure of the AcrAB-TolC multidrug efflux pump. Nature 509:512-515. http://dx.doi.org/10.1038/nature13205.
9. Symmons MF, Marshall RL, Bavro VN. 2015. Architecture and roles of periplasmic adaptor proteins in tripartite efflux assemblies. Front Microbiol 6:513. http://dx.doi.org/10.3389/fmicb.2015.00513.
10. Nikaido H, Zgurskaya HI. 2001. AcrAB and related multidrug efflux pumps of Escherichia coli. J Mol Microbiol Biotechnol 3:215-218.
11. Weeks JW, Nickels LM, Ntreh AT, Zgurskaya HI. 2015. Non-equivalent roles of two periplasmic subunits in the function and assembly of triclosan pump TriABC from Pseudomonas aeruginosa. Mol Microbiol 98:343-356. http://dx.doi.org/10.1111/mmi.13124.
12. Mima T, Joshi S, Gomez-Escalada M, Schweizer HP. 2007. Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. J Bacteriol 189:7600-7609. http://dx.doi.org/10.1128/JB.00850-07.
13. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. 2005. A Tn7-based broad-range bacterial cloning and expression system. Nat Methods 2:443-448. http://dx.doi.org/10.1038/nmeth765.
14. Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, Goldberg JB. 2013. Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of Gram-negative bacteria and single-copy promoter lux reporter analysis. Appl Environ Microbiol 79:4149-4153. http://dx.doi.org/10.1128/AEM.00640-13.
15. Vogel HJ, Bonner DM. 1956. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218:97-106.
16. de Lorenzo V, Timmis KN. 1994. Analysis and construction of stable phenotypes in Gram-negative bacteriawith Tn5- and Tn10-derived minitransposons. Methods Enzymol 235:386-405. http://dx.doi.org/10.1016/0076-6879(94)35157-0.
17. Tikhonova EB, Wang Q, Zgurskaya HI. 2002. Chimeric analysis of the multicomponent multidrug efflux transporters from Gram-negative bacteria. J Bacteriol 184:6499-6507. http://dx.doi.org/10.1128/JB.184.23.6499-6507.2002.
18. Xu Y, Sim SH, Song S, Piao S, Kim HM, Jin XL, Lee K, Ha NC. 2010. The tip region of the MacA alpha-hairpin is important for the binding to TolC to the Escherichia coli MacAB-TolC pump. Biochem Biophys Res Commun 394:962-965. http://dx.doi.org/10.1016/j.bbrc.2010.03.097.
19. Kim H-M, Xu Y, Lee M, Piao S, Sim S-H, Ha N-C, Lee K. 2010. Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC. J Bacteriol 192:4498-4503. http://dx.doi.org/10.1128/JB.00334-10.
20. Kamisetty H, Ovchinnikov S, Baker D. 2013. Assessing the utility of coevolution-based residue-residue contact predictions in a sequence- and structure-rich era. Proc Natl Acad Sci U S A 110:15674-15679. http://dx.doi.org/10.1073/pnas.1314045110.
21. Ovchinnikov S, Kamisetty H, Baker D. 2014. Robust and accurate prediction of residue-residue interactions across protein interfaces using evolutionary information. eLife 3:e02030. http://dx.doi.org/10.7554/eLife.02030.
22. Lobedanz S, Bokma E, Symmons MF, Koronakis E, Hughes C, Koronakis V. 2007. A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps. Proc Natl Acad Sci U S A 104:4612-4617. http://dx.doi.org/10.1073/pnas.0610160104.
23. Weeks JW, Celaya-Kolb T, Pecora S, Misra R. 2010. AcrA suppressor alterations reverse the drug hypersensitivity phenotype of a TolC mutant by inducing TolC aperture opening. Mol Microbiol 75:1468-1483. http://dx.doi.org/10.1111/j.1365-2958.2010.07068.x.
24. Pei XY, Hinchliffe P, Symmons MF, Koronakis E, Benz R, Hughes C, Koronakis V. 2011. Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Proc Natl Acad Sci U S A 108: 2112-2117. http://dx.doi.org/10.1073/pnas.1012588108.
25. Chuanchuen R, Murata T, Gotoh N, Schweizer HP. 2005. Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump. Antimicrob Agents Chemother 49:2133-2136. http://dx.doi.org/10.1128/AAC.49.5.2133-2136.2005.
26. Watson JM, Holloway BW. 1978. Linkage map of Pseudomonas aeruginosa PAT. J Bacteriol 136:507-521.
27. Schweizer HP. 2001. Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads. Curr Opin Biotechnol 12: 439-445. http://dx.doi.org/10.1016/S0958-1669(00)00242-1.