Matchout deuterium labelling of proteins for small-angle neutron scattering studies using prokaryotic and eukaryotic expression systems and high cell-density cultures

European Biophysics Journal

Volumn 46, Issue 5, 2017, Pages 425-432

  Dunne, O ^a,b   Weidenhaupt, M ^c   Callow, P ^b   Martel, A ^b   Moulin, M ^b   Perkins, S J ^a   Haertlein, M ^b   Forsyth, V T ^b,d  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b INSTITUT LAUE LANGEVIN   (France)

^c UNIV GRENOBLE ALPES   (France)

^d KEELE UNIVERSITY   (United Kingdom)

Author keywords

Contrast variation; Matchout deuteration; Neutron scattering; SANS

Indexed keywords

BACTERIAL PROTEIN; DEUTERIUM; FUNGAL PROTEIN; COMPLEMENT FACTOR H; MALTOSE BINDING PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; DENSITY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HYDROGENATION; KOMAGATAELLA PASTORIS; NEUTRON SCATTERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; BIOSYNTHESIS; CELL COUNT; CHEMISTRY; GENE EXPRESSION; GENETIC ENGINEERING; GENETICS; ISOLATION AND PURIFICATION; ISOTOPE LABELING; NEUTRON DIFFRACTION; PICHIA; PROCEDURES; SMALL ANGLE SCATTERING;

CELL COUNT; CELLS, CULTURED; COMPLEMENT FACTOR H; DEUTERIUM; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; ISOTOPE LABELING; MALTOSE-BINDING PROTEINS; NEUTRON DIFFRACTION; PICHIA; RECOMBINANT PROTEINS; SCATTERING, SMALL ANGLE;

EID: 84995421801     PISSN: 01757571     EISSN: 14321017     Source Type: Journal    
DOI: 10.1007/s00249-016-1186-2     Document Type: Article

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