Image cytofluorometry for the quantification of ploidy and endoplasmic reticulum stress in cancer cells

Methods in Molecular Biology

Volumn 1524, Issue , 2016, Pages 53-64

  Senovilla, Laura ^a   Demont, Yohann ^a   Humeau, Juliette ^a   Bloy, Norma ^a   Kroemer, Guido ^a  

^a CENTRE DE RECHERCHE DES CORDELIERS   (France)

Author keywords

Calreticulin; eIF2 ; ER stress; Image cytofluorometry; Tetraploidy

Indexed keywords

CALRETICULIN; DNA; INITIATION FACTOR 2ALPHA; NOCODAZOLE;

ANIMAL CELL; CANCER CELL LINE; CELL MEMBRANE; CELL SURFACE; COLON CARCINOMA; CONTROLLED STUDY; CYTOFLUOROMETRY; DNA CONTENT; ENDOPLASMIC RETICULUM STRESS; IMAGE CYTOMETRY; MOUSE; NONHUMAN; PLOIDY; PROTEIN PHOSPHORYLATION; SINGLE CELL ANALYSIS; TUMOR IMMUNITY; ANIMAL; CHICKEN; FLOW CYTOMETRY; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; LEPORIDAE; METABOLISM; NEOPLASM; PHYSIOLOGY; PROCEDURES;

ANIMALS; CHICKENS; ENDOPLASMIC RETICULUM STRESS; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; NEOPLASMS; PLOIDIES; RABBITS;

EID: 84994525502     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6603-5_3     Document Type: Chapter

References (21)

1. Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144: 646-674
2. Davoli T, de Lange T (2011) The causes and consequences of polyploidy in normal development and cancer. Annu Rev Cell Dev Biol 27: 585-610
3. Shackney SE, Smith CA, Miller BW, Burholt DR, Murtha K, Giles HR, Ketterer DM, Pollice AA (1989) Model for the genetic evolution of human solid tumors. Cancer Res 49: 3344-3354
4. Ganem NJ, Storchova Z, Pellman D (2007) Tetraploidy, aneuploidy and cancer. Curr Opin Genet Dev 17: 157-162
5. Vitale I, Galluzzi L, Castedo M, Kroemer G (2011) Mitotic catastrophe: a mechanism for avoiding genomic instability. Nat Rev Mol Cell Biol 12: 385-392
6. Vitale I, Galluzzi L, Senovilla L, Criollo A, Jemaa M, Castedo M et al (2011) Illicit survival of cancer cells during polyploidization and depolyploidization. Cell Death Differ 18: 1403-1413
7. Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, Pellman D (2005) Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437: 1043-1047
8. Andreassen PR, Lohez OD, Lacroix FB, Margolis RL (2001) Tetraploid state induces p53-dependent arrest of nontransformed mammalian cells in G1. Mol Biol Cell 12: 1315-1328
9. Senovilla L, Vitale I, Martins I, Tailler M, Pailleret C, Michaud M et al (2012) An immunosurveillance mechanism controls cancer cell ploidy. Science 337: 1678-1684
10. Finn OJ (2012) Immuno-oncology: understanding the function and dysfunction of the immune system in cancer. Ann Oncol 23(Suppl 8): viii6-viii9
11. Fridman WH, Pages F, Sautes-Fridman C, Galon J (2012) The immune contexture in human tumours: impact on clinical outcome. Nat Rev Cancer 12: 298-306
12. Kroemer G, Galluzzi L, Kepp O, Zitvogel L (2013) Immunogenic cell death in cancer therapy. Annu Rev Immunol 31: 51-72
13. Senovilla L, Vitale I, Martins I, Kepp O, Galluzzi L, Zitvogel L et al (2013) An anticancer therapy-elicited immunosurveillance system that eliminates tetraploid cells. Oncoimmunology 2: e22409
14. Senovilla L, Galluzzi L, Castedo M, Kroemer G (2013) Immunological control of cell cycle aberrations for the avoidance of oncogenesis: the case of tetraploidy. Ann N Y Acad Sci 1284: 57-61
15. Zitvogel L, Galluzzi L, Smyth MJ, Kroemer G (2013) Mechanism of action of conventional and targeted anticancer therapies: reinstating immunosurveillance. Immunity 39: 74-88
16. Galluzzi L, Buque A, Kepp O, Zitvogel L, Kroemer G (2015) Immunological effects of conventional chemotherapy and targeted anticancer agents. Cancer Cell 28: 690-714
17. Bloy N, Sauvat A, Chaba K, Buque A, Humeau J, Bravo-San Pedro JM et al (2015) Morphometric analysis of immunoselection against hyperploid cancer cells. Oncotarget 6: 41204-41215
18. Kepp O, Galluzzi L, Lipinski M, Yuan J, Kroemer G (2011) Cell death assays for drug discovery. Nat Rev Drug Discov 10: 221-237
19. Michalak M, Milner RE, Burns K, Opas M (1992) Calreticulin. Biochem J 285(Pt 3): 681-692
20. Obeid M, Tesniere A, Ghiringhelli F, Fimia GM, Apetoh L, Perfettini JL et al (2007) Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat Med 13: 54-61
21. Boileve A, Senovilla L, Vitale I, Lissa D, Martins I, Metivier D et al (2013) Immunosurveillance against tetraploidization- induced colon tumorigenesis. Cell Cycle 12: 473-479