Effect of phosphate availability on cyanophycin accumulation in Synechocystis sp. PCC 6803 and the production strain BW86

Algal Research

Volumn 20, Issue , 2016, Pages 189-196

  Trautmann, Andreas ^a   Watzer, Björn ^b   Wilde, Annegret ^c   Forchhammer, Karl ^b   Posten, Clemens ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Cyanobacteria; Cyanophycin; Phosphate starvation; Photobioreactor

Indexed keywords

EID: 84992727827     PISSN: 22119264     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.algal.2016.10.009     Document Type: Article

References (42)

1. [1] Wu, G., Wu, Q., Shen, Z., Accumulation of poly-β-hydroxybutyrate in cyanobacterium Synechocystis sp. PCC6803. Bioresour. Technol. 76:2 (2001), 85–90.
2. [2] Beck, C., Knoop, H., Axmann, I.M., Steuer, R., The diversity of cyanobacterial metabolism: genome analysis of multiple phototrophic microorganisms. BMC Genomics, 13, 2012.
3. [3] Ziegler, K., Deutzmann, R., Lockau, W., Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense. Zeitschrift für Naturforschung Section C-A Journal of Biosciences 57:5–6 (2002), 522–529.
4. [4] Allen, M.M., [19] Inclusions: Cyanophycin. Methods Enzymol. 167:C (1988), 207–213.
5. [5] Simon, R.D., The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue-green alga Anabaena cylindrica. Arch. Mikrobiol. 92:2 (1973), 115–122.
6. [6] Allen, M.M., Hawley, M.A., Protein degradation and synthesis of cyanophycin granule polypeptide in Aphanocapsa sp. J. Bacteriol. 154:3 (1983), 1480–1484.
7. [7] Lawry, N.H., Simon, R.D., The normal and induced occurence of cyanophycin inclusion-bodies in several blue-green-algae. J. Phycol. 18:3 (1982), 391–399.
8. [8] Schwamborn, M., Chemical synthesis of polyaspartates: a biodegradable alternative to currently used polycarboxylate homo- and copolymers. Polym. Degrad. Stab. 59:1 (1998), 39–45.
9. [9] Alford, D.D., Wheeler, A.P., Pettigrew, C.A., Biodegradation of thermally synthesized polyaspartate. J Environ Polymer Degrad 2:4 (1994), 225–236.
10. [10] Sallam, A., Steinbüchel, A., Dipeptides in nutrition and therapy: cyanophycin-derived dipeptides as natural alternatives and their biotechnological production. Appl. Microbiol. Biotechnol. 87:3 (2010), 815–828.
11. [11] Allen, M.M., Smith, A., Nitrogen chlorosis in blue-green algae. Arch. Mikrobiol. 69:2 (1969), 114–120.
12. [12] Allen, M.M., Cyanobacterial cell inclusions. Annu. Rev. Microbiol. 38 (1984), 1–25.
13. [13] Simon, R.D., Cyanophycin granules from the blue-green alga Anabaena cylindrica: a reserve material consisting of copolymers of aspartic acid and arginine. Proc. Natl. Acad. Sci. 68.2 (1971), 265–267.
14. [14] Simon, R.D., Weathers, P., Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria. Biochim. Biophys. Acta, Protein Struct. 420:1 (1976), 165–176.
15. [15] Lang, N.J., Simon, R.D., Wolk, C.P., Correspondence of cyanophycin granules with structured granules in Anabaena cylindrica. Arch. Mikrobiol. 83:4 (1972), 313–320.
16. [16] Allen, M.M., Weathers, P.J., Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308. J. Bacteriol. 141:2 (1980), 959–962.
17. [17] Obst, M., Oppermann-Sanio, F.B., Luftmann, H., Steinbüchel, A., Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI - The cphE gene from P. anguilliseptica BI encodes a cyanophycin-hydrolyzing enzyme. J. Biol. Chem. 277:28 (2002), 25096–25105.
18. [18] Li, H., Sherman, D., Bao, S., Sherman, L., Pattern of cyanophycin accumulation in nitrogen-fixing and non-nitrogen-fixing cyanobacteria. Arch. Microbiol. 176:1–2 (2001), 9–18.
19. [19] Picossi, S., Valladares, A., Flores, E., Herrero, A., Nitrogen-regulated genes for the metabolism of cyanophycin, a bacterial nitrogen reserve polymer - Expression and mutational analysis of two cyanophycin synthetase and cyanophycinase gene clusters in the heterocyst-forming cyanobacterium Anabaena sp PCC 7120. J. Biol. Chem. 279:12 (2004), 11582–11592.
20. [20] Obst, M., Sallam, A., Luftmann, H., Steinbüchel, A., Isolation and characterization of gram-positive cyanophycin-degrading bacteria - Kinetic studies on cyanophycin depolymerase activity in aerobic bacteria. Biomacromolecules 5:1 (2003), 153–161.
21. [21] Richter, R., Hejazi, M., Kraft, R., Ziegler, K., Lockau, W., Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme. Eur. J. Biochem. 263:1 (1999), 163–169.
22. [22] Obst, M., Steinbüchel, A., Microbial degradation of poly(amino acid)s. Biomacromolecules 5:4 (2004), 1166–1176.
23. [23] Simon, R.D., Measurement of the cyanophycin granule polypeptide contained in the blue-green alga anabaena cylindrica. J. Bacteriol. 114:3 (1973), 1213–1216.
24. [24] Elbahloul, Y., Krehenbrink, M., Reichelt, R., Steinbüchel, A., Physiological conditions conducive to high cyanophycin content in biomass of Acinetobacter calcoaceticus strain ADP1. Appl. Environ. Microbiol. 71.2 (2005), 858–866.
25. [25] Maheswaran, M., Ziegler, K., Lockau, W., Hagemann, M., Forchhammer, K., PII-regulated arginine synthesis controls accumulation of cyanophycin in Synechocystis sp. strain PCC 6803. J. Bacteriol. 188:7 (2006), 2730–2734.
26. [26] Forchhammer, K., Global carbon/nitrogen control by PII signal transduction in cyanobacteria: from signals to targets. FEMS Microbiol. Rev. 28:3 (2004), 319–333.
27. [27] Zeth, K., Fokinas, O., Forchhammers, K., Structural basis and target-specific modulation of ADP sensing by the Synechococcus elongatus PII signaling protein. J. Biol. Chem. 289:13 (2014), 8960–8972.
28. [28] Forchhammer, K., PII signal transducers: novel functional and structural insights. Trends Microbiol. 16:2 (2008), 65–72.
29. [29] Fokina, O., Chellamuthu, V.-R., Forchhammer, K., Zeth, K., Mechanism of 2-oxoglutarate signaling by the Synechococcus elongatus P-II signal transduction protein. Proc. Natl. Acad. Sci. 107:46 (2010), 19760–19765.
30. [30] Zeth, K., Fokina, O., Forchhammer, K., An engineered PII protein variant that senses a novel ligand. Acta Crystallogr. Sect. D 68:8 (2012), 901–908.
31. [31] Heinrich, A., Maheswaran, M., Ruppert, U., Forchhammer, K., The Synechococcus elongatus PII signal transduction protein controls arginine synthesis by complex formation with N-acetyl-L-glutamate kinase. Mol. Microbiol. 52:5 (2004), 1303–1314.
32. [32] Watzer, B., Engelbrecht, A., Hauf, W., Stahl, M., Maldener, I., Forchhammer, K., Metabolic pathway engineering using the central signal processor PII. Microb. Cell Factories, 14(1), 2015, 192.
33. [33] Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M., Stanier, R.Y., Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1 (1979), 1–61.
34. [34] Dillschneider, R., Steinweg, C., Rosello-Sastre, R., Posten, C., Biofuels from microalgae: Photoconversion efficiency during lipid accumulation. Bioresour. Technol. 142 (2013), 647–654.
35. [35] Messineo, L., Modification of the Sakaguchi reaction: Spectrophotometric determination of arginine in proteins without previous hydrolysis. Arch. Biochem. Biophys. 117:3 (1966), 534–540.
36. [36] Touloupakis, E., Cicchi, B., Torzillo, G., A bioenergetic assessment of photosynthetic growth of Synechocystis sp. PCC 6803 in continuous cultures. Biotechnol. Biofuels, 8, 2015, 133.
37. [37] Dauta, A., Devaux, J., Piquemal, F., Boumnich, L., Growth rate of four freshwater algae in relation to light and temperature. Hydrobiologia 207:1 (1990), 221–226.
38. [38] Bland, E., Angenent, L.T., Pigment-targeted light wavelength and intensity promotes efficient photoautotrophic growth of Cyanobacteria. Bioresour. Technol. 216 (2016), 579–586.
39. [39] Schlagermann, P., Göttlicher, G., Dillschneider, R., Rosello-Sastre, R., Posten, C., Composition of algal oil and its potential as biofuel. J. Comb., 2012.
40. [40] Collier, J.L., Grossman, A.R., A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria. EMBO J. 13:5 (1994), 1039–1047.
41. [41] Stevens, S.E., Paone, D.A., Accumulation of cyanophycin granules as a result of phosphate limitation in Agmenellum quadruplicatum. Plant Physiol. 67:4 (1981), 716–719.
42. [42] Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., Jones, P.R., Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), 2012, e50470.