Multivalent chromosomal expression of the Clostridium botulinum serotype A neurotoxin heavy-chain antigen and the Bacillus anthracis protective antigen in Lactobacillus acidophilus

Applied and Environmental Microbiology

Volumn 82, Issue 20, 2016, Pages 6091-6101

  O'Flaherty, Sarah ^a   Klaenhammer, Todd R ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; ANTIGENS; BACILLI; BACTERIA; BACTERIOLOGY; CHAINS; CHROMOSOMES; CLOSTRIDIUM; DNA; ELECTROPHORESIS; GENES; LACTIC ACID; PEPTIDES; TRANSCRIPTION; VACCINES;

CHROMOSOMAL REGIONS; CLOSTRIDIUM BOTULINUM; HETEROLOGOUS EXPRESSION; LACTIC ACID BACTERIA; LACTOBACILLUS ACIDOPHILUS; RECEPTOR-BINDING DOMAINS; SELECTIVE MAINTENANCE; WESTERN-BLOT ANALYSIS;

GENE EXPRESSION;

ANTIBIOTICS; ANTIGEN; BACTERIUM; CHROMOSOME; GENE EXPRESSION; PATHOGEN; PEPTIDE; PLASMID; PROTEIN; TOXIN; VACCINE;

BACILLUS ANTHRACIS; CLOSTRIDIUM BOTULINUM; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS ACIDOPHILUS NCFM;

BACTERIAL VACCINE; BOTULINUM TOXIN A;

ANTHRAX; BACILLUS ANTHRACIS; BACTERIAL CHROMOSOME; BOTULISM; CLOSTRIDIUM BOTULINUM; GENE EXPRESSION; GENETICS; LACTOBACILLUS ACIDOPHILUS; METABOLISM; MICROBIOLOGY;

ANTHRAX; BACILLUS ANTHRACIS; BACTERIAL VACCINES; BOTULINUM TOXINS, TYPE A; BOTULISM; CHROMOSOMES, BACTERIAL; CLOSTRIDIUM BOTULINUM; GENE EXPRESSION; LACTOBACILLUS ACIDOPHILUS;

EID: 84991272134     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01533-16     Document Type: Article

References (38)

1. Wells JM, Mercenier A. 2008. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat Rev Microbiol 6:349-362. http://dx.doi.org/10.1038/nrmicro1840.
2. Sanders ME, Klaenhammer TR. 2001. Invited review: the scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic. J Dairy Sci 84:319-331. http://dx.doi.org/10.3168/jds.S0022-0302(01)74481-5.
3. Konstantinov SR, Smidt H, de Vos WM, Bruijns SC, Singh SK, Valence F, Molle D, Lortal S, Altermann E, Klaenhammer TR, van Kooyk Y. 2008. S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions. Proc Natl Acad Sci U S A 105:19474-19479. http://dx.doi.org/10.1073/pnas.0810305105.
4. Mohamadzadeh M, Duong T, Sandwick SJ, Hoover T, Klaenhammer TR. 2009. Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge. Proc Natl Acad Sci U S A 106:4331-4336. http://dx.doi.org/10.1073/pnas.0900029106.
5. Curiel TJ, Morris C, Brumlik M, Landry SJ, Finstad K, Nelson A, Joshi V, Hawkins C, Alarez X, Lackner A, Mohamadzadeh M. 2004. Peptides identified through phage display direct immunogenic antigen to dendritic cells. J Immunol 172:7425-7431. http://dx.doi.org/10.4049/jimmunol.172.12.7425.
6. Kajikawa A, Nordone SK, Zhang L, Stoeker LL, LaVoy AS, Klaenhammer TR, Dean GA. 2011. Dissimilar properties of two recombinant Lactobacillus acidophilus strains displaying Salmonella FliC with different anchoring motifs. Appl Environ Microbiol 77:6587-6596. http://dx.doi.org/10.1128/AEM.05153-11.
7. Kajikawa A, Zhang L, Long J, Nordone S, Stoeker L, LaVoy A, Bumgardner S, Klaenhammer T, Dean G. 2012. Construction and immunological evaluation of dual cell surface display of HIV-1 gag and Salmonella enterica serovar Typhimurium FliC in Lactobacillus acidophilus for vaccine delivery. Clin Vaccine Immunol 19:1374-1381. http://dx.doi.org/10.1128/CVI.00049-12.
8. Douglas GL, Goh YJ, Klaenhammer TR. 2011. Integrative food grade expression system for lactic acid bacteria. Methods Mol Biol 765:373-387. http://dx.doi.org/10.1007/978-1-61779-197-0_22.
9. Douglas GL, Klaenhammer TR. 2011. Directed chromosomal integration and expression of the reporter gene gusA3 in Lactobacillus acidophilus NCFM. Appl Environ Microbiol 77:7365-7371. http://dx.doi.org/10.1128/AEM.06028-11.
10. Gill DM. 1982. Bacterial toxins: a table of lethal amounts. Microbiol Rev 46:86-94.
11. Centers for Disease Control and Prevention. 2011. Notice of CDC's discontinuation of investigational pentavalent (ABCDE) botulinum toxoid vaccine for workers at risk for occupational exposure to botulinum toxins. MMWR Morb Mortal Wkly Rep 60:1454-1455.
12. Aoki KR, Smith LA, Atassi MZ. 2010. Mode of action of botulinum neurotoxins: current vaccination strategies and molecular immune recognition. Crit Rev Immunol 30:167-187. http://dx.doi.org/10.1615/CritRevImmunol.v30.i2.50.
13. Pondo T, Rose CE, Jr, Martin SW, Keitel WA, Keyserling HL, Babcock J, Parker S, Jacobson RM, Poland GA, McNeil MM. 2014. Evaluation of sex, race, body mass index and pre-vaccination serum progesterone levels and post-vaccination serum anti-anthrax protective immunoglobulin G on injection site adverse events following anthrax vaccine adsorbed (AVA) in the CDC AVA human clinical trial. Vaccine 32:3548-3554. http://dx.doi.org/10.1016/j.vaccine.2014.04.025.
14. Kimmel SA, Roberts RF. 1998. Development of a growth medium suitable for exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus RR. Int J Food Microbiol 40:87-92. http://dx.doi.org/10.1016/S0168-1605(98)00023-3.
15. Sambrook J, Russel DW. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Hanahan D. 1985. Techniques for transformation of E. coli, p 109-135. In GloverDM(ed), DNA cloning: a practical approach, vol 1. IRL Press Ltd., Oxford, England.
17. Walker DC, Aoyama K, Klaenhammer TR. 1996. Electrotransformation of Lactobacillus acidophilus group A1. FEMS Microbiol Lett 138:233-237. http://dx.doi.org/10.1111/j.1574-6968.1996.tb08163.x.
18. Grote A, Hiller K, Scheer M, Munch R, Nortemann B, Hempel DC, Jahn D. 2005. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Res 33:W526-W531. http://dx.doi.org/10.1093/nar/gki376.
19. Goh YJ, Azcarate-Peril MA, O'Flaherty S, Durmaz E, Valence F, Jardin J, Lortal S, Klaenhammer TR. 2009. Development and application of a upp-based counterselective gene replacement system for the study of the S-layer protein SlpX of Lactobacillus acidophilus NCFM. Appl Environ Microbiol 75:3093-3105. http://dx.doi.org/10.1128/AEM.02502-08.
20. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647-1649. http://dx.doi.org/10.1093/bioinformatics/bts199.
21. Clayton MA, Clayton JM, Brown DR, Middlebrook JL. 1995. Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli. Infect Immun 63:2738-2742.
22. Maddaloni M, Staats HF, Mierzejewska D, Hoyt T, Robinson A, Callis G, Kozaki S, Kiyono H, McGhee JR, Fujihashi K, Pascual DW. 2006. Mucosal vaccine targeting improves onset of mucosal and systemic immunity to botulinum neurotoxin A. J Immunol 177:5524-5532. http://dx.doi.org/10.4049/jimmunol.177.8.5524.
23. Simpson L. 2013. The life history of a botulinum toxin molecule. Toxicon 68:40-59. http://dx.doi.org/10.1016/j.toxicon.2013.02.014.
24. Kiyatkin N, Maksymowych AB, Simpson LL. 1997. Induction of an immune response by oral administration of recombinant botulinum toxin. Infect Immun 65:4586-4591.
25. Park JB, Simpson LL. 2003. Inhalational poisoning by botulinum toxin and inhalation vaccination with its heavy-chain component. Infect Immun 71:1147-1154. http://dx.doi.org/10.1128/IAI.71.3.1147-1154.2003.
26. Ravichandran E, Al-Saleem FH, Ancharski DM, Elias MD, Singh AK, Shamim M, Gong Y, Simpson LL. 2007. Trivalent vaccine against botulinum toxin serotypes A, B, and E that can be administered by the mucosal route. Infect Immun 75:3043-3054. http://dx.doi.org/10.1128/IAI.01893-06.
27. O'Flaherty S, Klaenhammer TR. 2011. The impact of omic technologies on the study of food microbes. Annu Rev Food Sci Technol 2:353-371. http://dx.doi.org/10.1146/annurev-food-030810-110338.
28. Mohamadzadeh M, Pfeiler EA, Brown JB, Zadeh M, Gramarossa M, Managlia E, Bere P, Sarraj B, Khan MW, Pakanati KC, Ansari MJ, O'Flaherty S, Barrett T, Klaenhammer TR. 2011. Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid. Proc Natl Acad Sci U S A 108(Suppl 1):4623-4630. http://dx.doi.org/10.1073/pnas.1005066107.
29. Khazaie K, Zadeh M, Khan MW, Bere P, Gounari F, Dennis K, Blatner NR, Owen JL, Klaenhammer TR, Mohamadzadeh M. 2012. Abating colon cancer polyposis by Lactobacillus acidophilus deficient in lipoteichoic acid. Proc Natl Acad Sci U S A 109:10462-10467. http://dx.doi.org/10.1073/pnas.1207230109.
30. Mohamadzadeh M, Durmaz E, Zadeh M, Pakanati KC, Gramarossa M, Cohran V, Klaenhammer TR. 2010. Targeted expression of anthrax protective antigen by Lactobacillus gasseri as an anthrax vaccine. Future Microbiol 5:1289-1296. http://dx.doi.org/10.2217/fmb.10.78.
31. Barrangou R, Azcarate-Peril MA, Duong T, Conners SB, Kelly RM, Klaenhammer TR. 2006. Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays. Proc Natl Acad Sci U S A 103:3816-3821. http://dx.doi.org/10.1073/pnas.0511287103.
32. Johnson BR, Hymes J, Sanozky-Dawes R, Henriksen ED, Barrangou R, Klaenhammer TR. 2016. Conserved S-layer-associated proteins revealed by exoproteomic survey of S-layer-forming lactobacilli. Appl Environ Microbiol 82:134-145. http://dx.doi.org/10.1128/AEM.01968-15.
33. Liu X, Lagenaur LA, Simpson DA, Essenmacher KP, Frazier-Parker CL, Liu Y, Tsai D, Rao SS, Hamer DH, Parks TP, Lee PP, Xu Q. 2006. Engineered vaginal Lactobacillus strain for mucosal delivery of the human immunodeficiency virus inhibitor cyanovirin-N. Antimicrob Agents Chemother 50:3250-3259. http://dx.doi.org/10.1128/AAC.00493-06.
34. Gervasi T, Horn N, Wegmann U, Dugo G, Narbad A, Mayer MJ. 2014. Expression and delivery of an endolysin to combat Clostridium perfringens. Appl Microbiol Biotechnol 98:2495-2505. http://dx.doi.org/10.1007/s00253-013-5128-y.
35. Casadaban MJ, Cohen SN. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179-207. http://dx.doi.org/10.1016/0022-2836(80)90283-1.
36. Duong T, Miller MJ, Barrangou R, Azcarate-Peril MA, Klaenhammer TR. 2011. Construction of vectors for inducible and constitutive gene expression in Lactobacillus. Microb Biotechnol 4:357-367. http://dx.doi.org/10.1111/j.1751-7915.2010.00200.x.
37. Law J, Buist G, Haandrikman A, Kok J, Venema G, Leenhouts K. 1995. A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes. J Bacteriol 177:7011-7018.
38. Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J. 1996. A general system for generating unlabelled gene replacements in bacterial chromosomes. Mol Gen Genet 253:217-224. http://dx.doi.org/10.1007/s004380050315.