A Temporal threshold for formaldehyde crosslinking and fixation

PLoS ONE

Volumn 4, Issue 2, 2009, Pages

  Schmiedeberg, Lars ^a   Skene, Pete ^a   Deaton, Aimée ^a   Bird, Adrian ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; METHYL CPG BINDING PROTEIN 2;

ANIMAL CELL; ARTICLE; BLEACHING; CHROMATIN IMMUNOPRECIPITATION; CROSS LINKING; FORMALDEHYDE CROSSLINKING; FORMALDEHYDE FIXATION; IN VIVO STUDY; INVESTIGATIVE PROCEDURES; LIVE CELL IMAGING; MICROSCOPY; MOUSE; NONHUMAN; PROTEIN DNA INTERACTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; TIME FACTOR;

EID: 84990251443     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004636     Document Type: Article

References (18)

1. Solomon MJ, Larsen PL, Varshavsky A (1988) Mapping protein-DNA interactions in vivo with formaldehyde: evidence that histone H4 is retained on a highly transcribed gene. Cell 53: 937-47.
2. Solomon MJ, Varshavsky A (1985) Formaldehyde-mediated DNA-protein crosslinking: a probe for in vivo chromatin structures. Proc Natl Acad Sci U S A 82: 6470-4.
3. Gilmour DS, Rougvie AE, Lis JT (1991) Protein-DNA cross-linking as a means to Determine the distribution of proteins on DNA in vivo. Methods in Cell Biology 35: 369-381.
4. Orlando V, Strutt H, Paro R (1997) Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods 11: 205-14.
5. Lewis JD, Meehan RR, Henzel WJ, Maurer-Fogy I, Jeppesen P, et al. (1992) Purification, sequence and cellular localisation of a novel chromosomal protein that binds to methylated DNA. Cell 69: 905-914.
6. Nan X, Tate P, Li E, Bird AP (1996) DNA Methylation specifies chromosomal localization of MeCP2. Molec and Cell Biol 16: 414-421.
7. Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, et al. (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpGbinding protein 2. Nature Genet 23: 185-188.
8. Kudo S, Nomura Y, Segawa M, Fujita N, Nakao M, et al. (2003) Heterogeneity in residual function of MeCP2 carrying missense mutations in the methyl CpG binding domain. J Med Genet 40: 487-93.
9. Klose RJ, Sarraf SA, Schmiedeberg L, McDermott SM, Stancheva I, et al. (2005) DNA binding specificity of MeCP2 due to a requirement for A/T sequences adjacent to methyl-CpG. Molecular Cell 19: 667-678.
10. Kumar A, Kamboj S, Malone BM, Kudo S, Twiss JL, et al. (2008) Analysis of protein domains and Rett syndrome mutations indicate that multiple regions influence chromatin-binding dynamics of the chromatin-associated protein MECP2 in vivo. J Cell Sci 121: 1128-37.
11. Nagaich AK, Walker DA, Wolford R, Hager GL (2004) Rapid periodic binding and displacement of the glucocorticoid receptor during chromatin remodeling. Mol Cell 14: 163-74.
12. Bosisio D, Marazzi I, Agresti A, Shimizu N, Bianchi ME, et al. (2006) A hyperdynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappaB-dependent gene activity. Embo J 25: 798-810.
13. Cherukuri S, Hock R, Ueda T, Catez F, Rochman M, et al. (2008) Cell cycledependent binding of HMGN proteins to chromatin. Mol Biol Cell 19: 1816-24.
14. Pallier C, Scaffidi P, Chopineau-Proust S, Agresti A, Nordmann P, et al. (2003) Association of chromatin proteins high mobility group box (HMGB) 1 and HMGB2 with mitotic chromosomes. Mol Biol Cell 14: 3414-26.
15. Ficz G, Heintzmann R, Arndt-Jovin DJ (2005) Polycomb group protein complexes exchange rapidly in living Drosophila. Development 132: 3963-76.
16. Klose RJ, Bird AP (2004) MeCP2 behaves as an elongated monomer that does not stably associate with the Sin3a chromatin remodelling complex. J Biol Chem.
17. Barr H, Hermann A, Berger J, Tsai HH, Adie K, et al. (2007) Mbd2 contributes to DNA methylation-directed repression of the xist gene. Mol Cell Biol 27: 3750-7.
18. Schmiedeberg L, Weisshart K, Diekmann S, Meyer Zu Hoerste G, Hemmerich P (2004) High-and low-mobility populations of HP1 in heterochromatin of mammalian cells. Mol Biol Cell 15: 2819-33.