Isolation and characterization of exosome from human embryonic stem cell-derived c-myc-immortalized mesenchymal stem cells

Methods in Molecular Biology

Volumn 1416, Issue , 2016, Pages 477-494

  Lai, Ruenn Chai ^a   Yeo, Ronne Wee Yeh ^a   Padmanabhan, Jayanthi ^b   Choo, Andre ^b,c   De Kleijn, Dominique P V ^d,e,f,g   Lim, Sai Kiang ^a,d  

^a INSTITUTE OF MEDICAL BIOLOGY   (Singapore)

^b AGENCY FOR SCIENCE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^e NATIONAL UNIVERSITY HEALTH SYSTEM   (Singapore)

^f UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^g INTERUNIVERSITY CARDIOLOGY INSTITUTE OF THE NETHERLANDS   (Netherlands)

Author keywords

Exosome; Immortalization; Mesenchymal stem cells

Indexed keywords

LENTIVIRUS VECTOR; MYC PROTEIN; PROTEIN; MYC PROTEIN, HUMAN;

CELL CLONING; CELL IMMORTALIZATION; CELL ISOLATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EXOSOME; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMAN EMBRYONIC STEM CELL; LIMIT OF QUANTITATION; MESENCHYMAL STEM CELL; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; STEM CELL EXPANSION; SUCROSE DENSITY GRADIENT CENTRIFUGATION; VIRAL GENE DELIVERY SYSTEM; CELL DIFFERENTIATION; CYTOLOGY; HEK293 CELL LINE; MESENCHYMAL STROMA CELL; METABOLISM;

CELL DIFFERENTIATION; EXOSOMES; HEK293 CELLS; HUMAN EMBRYONIC STEM CELLS; HUMANS; MESENCHYMAL STROMAL CELLS; PROTO-ONCOGENE PROTEINS C-MYC;

EID: 84990197797     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3584-0_29     Document Type: Chapter

References (29)

1. Mendicino M, Bailey AM, Wonnacott K et al (2014) MSC-based product characterization for clinical trials: an FDA perspective. Cell Stem Cell 14:141-145
2. Lai RC, Chen TS, Lim SK (2011) Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease. Regen Med 6:481-492
3. Gnecchi M, He H, Noiseux N et al (2006) Evidence supporting paracrine hypothesis for Akt-modified mesenchymal stem cell-mediated cardiac protection and functional improvement. FASEB J 20:661-669
4. Gnecchi M, He H, Noiseux N et al (2005) Paracrine action accounts for marked protection of ischemic heart by Akt-modified mesenchymal stem cells. Nat Med 11:367-368
5. Mirotsou M, Zhang Z, Deb A et al (2007) Secreted frizzled related protein 2 (Sfrp2) is the key Akt-mesenchymal stem cell-released paracrine factor mediating myocardial survival and repair. Proc Natl Acad Sci U S A 104:1643-1648
6. Timmers L, Lim SK, Arslan F et al (2007) Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Res 1:129-137
7. Lai RC, Arslan F, Lee MM et al (2010) Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res 4:214-222
8. Lai RC, Arslan F, Tan SS et al (2010) Derivation and characterization of human fetal MSCs: an alternative cell source for large-scale production of cardioprotective microparticles. J Mol Cell Cardiol 48:1215-1224
9. Bi B, Schmitt R, Israilova M et al (2007) Stromal cells protect against acute tubular injury via an endocrine effect. J Am Soc Nephrol 18:2486-2496
10. Parekkadan B, van Poll D, Suganuma K et al (2007) Mesenchymal stem cell-derived molecules reverse fulminant hepatic failure. PLoS One 2, e941
11. Timmers L, Lim SK, Hoefer IE et al (2011) Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction. Stem Cell Res 6: 206-214
12. Schafer R, Northoff H (2008) Cardioprotection and cardiac regeneration by mesenchymal stem cells. Panminerva Med 50:31-39
13. Haynesworth SE, Baber MA, Caplan AI (1996) Cytokine expression by human marrow-derived mesenchymal progenitor cells in vitro: effects of dexamethasone and IL-1 alpha. J Cell Physiol 166:585-592
14. Kinnaird T, Stabile E, Burnett MS et al (2004) Bone-marrow-derived cells for enhancing collateral development: mechanisms, animal data, and initial clinical experiences. Circ Res 95:354-363
15. Patschan D, Plotkin M, Goligorsky MS (2006) Therapeutic use of stem and endothelial progenitor cells in acute renal injury: ca ira. Curr Opin Pharmacol 6:176-183
16. Miyahara Y, Nagaya N, Kataoka M et al (2006) Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med 12:459-465
17. Min JY, Sullivan MF, Yang Y et al (2002) Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs. Ann Thorac Surg 74:1568-1575
18. Kinnaird T, Stabile E, Burnett MS et al (2004) Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circ Res 94:678-685
19. Sze SK, de Kleijn DP, Lai RC et al (2007) Elucidating the secretion proteome of human embryonic stem cell-derived mesenchymal stem cells. Mol Cell Proteomics 6:1680-1689
20. Kordelas L, Rebmann V, Ludwig AK et al (2014) MSC-derived exosomes: a novel tool to treat therapy-refractory graft-versus-host disease. Leukemia 28:970-973
21. Ludwig AK, Kordelas L, Rebmann V et al (2012) Exosomes-from bench to bedside. Klin Padiatr 224:A6
22. Xin H, Li Y, Buller B et al (2012) Exosomemediated transfer of miR-133b from multipotent mesenchymal stromal cells to neural cells contributes to neurite outgrowth. Stem Cells 30:1556-1564
23. Li T, Yan Y, Wang B et al (2013) Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis. Stem Cells Dev 22:845-854
24. Lee C, Mitsialis SA, Aslam M et al (2012) Exosomes mediate the cytoprotective action of mesenchymal stromal cells on hypoxia-induced pulmonary hypertension. Circulation 126: 2601-2611
25. Reis LA, Borges FT, Simões MJ et al (2012) Bone marrow-derived mesenchymal stem cells repaired but did not prevent gentamicininduced acute kidney injury through paracrine effects in rats. PLoS One 7:e44092
26. Tan CY, Lai RC, Wong W et al (2014) Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models. Stem Cell Res Ther 5:76, Epub ahead of print
27. Thery C, Ostrowski M, Segura E (2009) Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 9:581-593
28. Pan BT, Johnstone RM (1983) Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor. Cell 33:967-978
29. Chen TS, Arslan F, Yin Y et al (2011) Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J Transl Med 9:47