Applying mini-bore HPAEC-MS/MS for the characterization and quantification of Fc N-glycans from heterogeneously glycosylated IgGs

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 1033-1034, Issue , 2016, Pages 342-352

  Maier, Maria ^a   Reusch, Dietmar ^a   Bruggink, Cees ^b   Bulau, Patrick ^a   Wuhrer, Manfred ^b   Mølhøj, Michael ^c  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c ROCHE INNOVATION CENTER MUNICH   (Germany)

Author keywords

HPAEC MS MS; IgG; Mass spectrometry; N Glycan; Oligosaccharides; Pulsed amperometric detection; Sialyltransferase

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; IONS; MASS SPECTROMETRY; OLIGOSACCHARIDES; SIGNAL TO NOISE RATIO; SPECTROMETRY;

DETECTION AND QUANTIFICATIONS; HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY; HPAEC-MS/MS; N-GLYCAN; PULSED AMPEROMETRIC DETECTION; QUANTITATIVE EVALUATION; RELATIVE QUANTIFICATION; SIALYLTRANSFERASE;

POLYSACCHARIDES;

FC N GLYCAN DERIVATIVE; GLYCAN DERIVATIVE; IMMUNOGLOBULIN G; MANNOSE; UNCLASSIFIED DRUG; ANION; GLYCOSYLATED IGG; N ACETYLNEURAMINIC ACID; POLYSACCHARIDE; SOLUTION AND SOLUBILITY;

ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ARTICLE; CHO CELL LINE; CHROMATOGRAPHY; ELECTROSPRAY ION TRAP MASS SPECTROMETRY; FEMALE; GLYCOSYLATION; HIGH PERFORMANCE ANION EXCHANGE CHROMATOGRAPHY; HUMAN; HYDROPHILIC INTERACTION CHROMATOGRAPHY; ION CURRENT CHROMATOGRAPHY; ION TRAP MASS SPECTROMETRY; LIMIT OF DETECTION; MYELOMA CELL; NONHUMAN; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; CALIBRATION; CHEMICAL PHENOMENA; CRICETULUS; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; METABOLISM; MOUSE; PROCEDURES; SOLUTION AND SOLUBILITY;

ANIMALS; ANIONS; CALIBRATION; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN G; LIMIT OF DETECTION; MICE; N-ACETYLNEURAMINIC ACID; POLYSACCHARIDES; SOLUTIONS; TANDEM MASS SPECTROMETRY;

EID: 84990178638     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2016.08.001     Document Type: Article

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