Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles - Part 1: Separation-based methods

mAbs

Volumn 7, Issue 1, 2015, Pages 167-179

  Reusch, Dietmar ^a   Haberger, Markus ^a   Maier, Bernd ^a   Maier, Maria ^a   Kloseck, Ronny ^a   Zimmermann, Boris ^a   Hook, Michaela ^a   Szabo, Zoltan ^b   Tep, Samnang ^b   Wegstein, Jo ^b   Alt, Nadja ^a   Bulau, Patrick ^a   Wuhrer, Manfred ^c,d  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b ProZyme Inc   (United States)

^c LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^d VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

2 ab labeling; Apts labeling; CE LIF; Dna analyzer; Glycan analysis; High throughput; HILIC UPLC; HPAEC; IgG glycosylation; Method comparison; Monoclonal antibody (mab)

Indexed keywords

2 AMINOBENZAMIDE; FLUORESCENT DYE; GLYCAN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G1 ANTIBODY; NAPHTHALENE DERIVATIVE; PYRENE DERIVATIVE; RECOMBINANT ANTIBODY; SIALIC ACID; SULFONIC ACID DERIVATIVE; MONOCLONAL ANTIBODY;

AMPEROMETRY; ANALYTIC METHOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOPROCESS; CAPILLARY ELECTROPHORESIS; CARBOHYDRATE ANALYSIS; ELECTROCHEMICAL DETECTION; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HYDROPHILIC INTERACTION CHROMATOGRAPHY; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEASUREMENT ACCURACY; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION; SEPARATION TECHNIQUE; SIALYLATION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; CHEMISTRY; CHO CELL LINE; COMPARATIVE STUDY; CRICETULUS; GLYCOSYLATION; HAMSTER;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; GLYCOSYLATION; IMMUNOGLOBULIN FC FRAGMENTS; MASS SPECTROMETRY;

EID: 84921395878     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/19420862.2014.986000     Document Type: Article

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