Disruption of membranes of extracellular vesicles is necessary for ELISA determination of urine AQP2: Proof of disruption and epitopes of AQP2 antibodies

International Journal of Molecular Sciences

Volumn 17, Issue 10, 2016, Pages

  Nameta, Masaaki ^a   Saijo, Yoko ^b   Ohmoto, Yasukazu ^b   Katsuragi, Kiyonori ^b   Yamamoto, Keiko ^a   Yamamoto, Tadashi ^a   Ishibashi, Kenichi ^c   Sasaki, Sei ^c,d  

^a NIIGATA UNIVERSITY   (Japan)

^b OTSUKA PHARMACEUTICAL CO LTD   (Japan)

^c MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

^d TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Aquaporin 2 (AQP2); Biomarker; ELISA; Exosome; Extracellular vesicle; Kidney; Water balance

Indexed keywords

AQUAPORIN 2; EPITOPE;

ARTICLE; BINDING ASSAY; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXOSOME; FLUID BALANCE; HUMAN; HUMAN CELL; TEMPERATURE; ULTRACENTRIFUGATION;

EID: 84989332702     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms17101634     Document Type: Article

References (18)

1. Sasaki, S. Aquaporin 2: From its discovery to molecular structure and medical implications. Mol. Asp. Med. 2012, 33, 535–546.
2. Radin, M.J.; Yu, M.J.; Stoedkilde, L.; Miller, R.L.; Hoffert, J.D.; Frokiaer, J.; Pisitkun, T.; Knepper, M.A. Aquaporin-2 regulation in health and disease. Vet. Clin. Pathol. 2012, 41, 455–470.
3. Noda, Y.; Sohara, E.; Ohta, E.; Sasaki, S. Aquaporins in kidney pathophysiology. Nat. Rev. Nephrol. 2010, 6, 168–178.
4. Kanno, K.; Sasaki, S.; Hirata, Y.; Ishikawa, S.; Fushimi, K.; Nakanishi, S.; Bichet, D.G.; Marumo, F. Urinary excretion of aquaporin-2 in patients with diabetes insipidus. N. Engl. J. Med. 1995, 332, 1540–1545.
5. Wen, H.; Frokiaer, J.; Kwon, T.H.; Nielsen, S. Urinary excretion of aquaporin-2 in rat is mediated by a vasopressin-dependent apical pathway. J. Am. Soc. Nephrol. 1999, 10, 1416–1429.
6. Pisitkun, T.; Shen, R.F.; Knepper, M.A. Identification and proteomic profiling of exosomes in human urine. Proc. Natl. Acad. Sci. USA 2004, 101, 13368–13373.
7. Ishikawa, S.E.; Schrier, R.W. Pathophysiological roles of arginine vasopressin and aquaporin-2 in impaired water excretion. Clin. Endocrinol. 2003, 58, 1–17.
8. Kortenoeven, M.L.; Fenton, R.A. Renal aquaporins and water balance disorders. Biochim. Biophys. Acta 2014, 1840, 1533–1549.
9. Imamura, T.; Kinugawa, K. Urine Aquaporin-2: A promising marker of response to the arginine vasopressin type-2 antagonist, tolvaptan in patients with congestive heart failure. Int. J. Mol. Sci. 2016, 14, 17.
10. Nakanishi, H.; Kurosaki, M.; Hosokawa, T.; Takahashi, Y.; Itakura, J.; Suzuki, S.; Yasui, Y.; Tamaki, N.; Nakakuki, N.; Takada, H.; et al. Urinary excretion of the water channel aquaporin 2 correlated with the pharmacological effect of tolvaptan in cirrhotic patients with ascites. J. Gastroenterol. 2016, 51, 620–627.
11. Sasaki, S.; Saijo, Y.; Ohmoto, Y.; Iwata, F.; Koga, D.; Katsuragi, K. Alkali treatment stabilizes fluctuations of urine AQP2 values measured by ELISA. Clin. Exp. Nephrol. 2015, 1–6.
12. Kowal, J.; Tkach, M.; Théry, C. Biogenesis and secretion of exosomes. Curr. Opin. Cell Biol. 2014, 29, 116–125.
13. Salih, M.; Zietse, R.; Hoorn, E.J. Urinary extracellular vesicles and the kidney: Biomarkers and beyond. Am. J. Physiol. Ren. Physiol. 2014, 306, F1251–F1259.
14. Salih, M.; Fenton, R.A.; Knipscheer, J.; Janssen, J.W.; Vredenbregt-van den Berg, M.S.; Jenster, G.; Zietse, R.; Hoorn, E.J. An immunoassay for urinary extracellular vesicles. Am. J. Physiol. Ren. Physiol. 2016, 310, 796–801.
15. Imamura, T.; Kinugawa, K.; Komuro, I. Tolvaptan prolongs blockage of the vasopressin Type II receptor over 24 hours in responders with stage D heart failure. Int. Heart J. 2016, 57, 41–46.
16. Frick, A.; Eriksson, U.K.; de Mattia, F.; Oberg, F.; Hedfalk, K.; Neutze, R.; de Grip, W.J.; Deen, P.M.; Törnroth-Horsefield, S. X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and trafficking. Proc. Natl. Acad. Sci. USA 2014, 111, 6305–6310.
17. Sasaki, S.; Ohmoto, Y.; Mori, T.; Iwata, F.; Muraguchi, M. Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method. Clin. Exp. Nephrol. 2012, 16, 406–410.
18. Zhou, H.; Yuen, P.S.; Pisitkun, T.; Gonzales, P.A.; Yasuda, H.; Dear, J.W.; Gross, P.; Knepper, M.A.; Star, R.A. Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int. 2006, 69, 1471–1476.