Metabolic evolution and 13C flux analysis of a succinate dehydrogenase deficient strain of Yarrowia lipolytica

Biotechnology and Bioengineering

Volumn 113, Issue 11, 2016, Pages 2425-2432

  Yuzbashev, Tigran V ^a   Bondarenko, Pavel Yu ^a   Sobolevskaya, Tatiana I ^a   Yuzbasheva, Evgeniya Yu ^a   Laptev, Ivan A ^a   Kachala, Vadim V ^b   Fedorov, Alexander S ^a   Vybornaya, Tatiana V ^a   Larina, Anna S ^a   Sineoky, Sergey P ^a  

^a STATE RESEARCH INSTITUTE OF GENETICS AND SELECTION OF INDUSTRIAL MICROORGANISMS   (Russian Federation)

^b N D ZELINSKY INSTITUTE OF ORGANIC CHEMISTRY   (Russian Federation)

Author keywords

low pH; metabolic evolution; SDH2; succinic acid; Yarrowia lipolytica

Indexed keywords

FERMENTATION; GLUCOSE; GLYCEROL;

COMBINATION APPROACHES; METABOLIC EVOLUTION; PENTOSE PHOSPHATE PATHWAY; SDH2; SUCCINATE DEHYDROGENASE; SUCCINIC ACIDS; TRICARBOXYLIC ACID CYCLE; YARROWIA LIPOLYTICA;

METABOLISM;

CARBON 13; GLUCOSE; GLYCEROL; SUCCINATE DEHYDROGENASE; SUCCINIC ACID;

ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL MUTAGENESIS; CITRIC ACID CYCLE; FUNGAL METABOLISM; FUNGAL STRAIN; METABOLIC FLUX ANALYSIS; NONHUMAN; OXIDATION; PENTOSE PHOSPHATE CYCLE; PROTON NUCLEAR MAGNETIC RESONANCE; QUANTITATIVE ANALYSIS; YARROWIA LIPOLYTICA;

EID: 84988939378     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26007     Document Type: Article

References (24)

1. Blank LM, Lehmbeck F, Sauer U. 2005. Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts. FEMS Yeast Res 5(6–7):545–558.
2. Camarasa C, Grivet JP, Dequin S. 2003. Investigation by 13C-NMR and tricarboxylic acid (TCA) deletion mutant analysis of pathways for succinate formation in Saccharomyces cerevisiae during anaerobic fermentation. Microbiology 149(Pt 9):2669–2678.
3. Cukalovic A, Stevens CV. 2008. Feasibility of production methods for succinic acid derivatives: A marriage of renewable resources and chemical technology. Biofuels Bioprod Bioref 2(6):505–529.
4. Dulermo R, Gamboa-Melendez H, Michely S, Thevenieau F, Neuveglise C, Nicaud JM. 2015. The evolution of Jen3 proteins and their role in dicarboxylic acid transport in Yarrowia. Microbiologyopen 4(1):100–120.
5. Flores CL, Gancedo C. 2005. Yarrowia lipolytica mutants devoid of pyruvate carboxylase activity show an unusual growth phenotype. Eukaryot Cell 4(2):356–364.
6. Goldberg I, Rokem JS, Pines O. 2006. Organic acids: Old metabolites, new themes. J Chemical Technol Biotechnol 81(10):1601–1611.
7. Jansen ML, van Gulik WM. 2014. Towards large scale fermentative production of succinic acid. Curr Opin Biotechnol 30:190–197.
8. Jantama K, Haupt MJ, Svoronos SA, Zhang X, Moore JC, Shanmugam KT, Ingram LO. 2008a. Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnol Bioeng 99(5):1140–1153.
9. Jantama K, Zhang X, Moore JC, Shanmugam KT, Svoronos SA, Ingram LO. 2008b. Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C. Biotechnol Bioeng 101(5):881–893.
10. Lupianez JA, Machado A, Nunez de Castro I, Mayor F. 1974. Succinic acid production by yeasts grown under different hypoxic conditions. Mol Cell Biochem 3(2):113–116.
11. Matsuoka M, Ueda Y, Aiba S. 1980. Role and control of isocitrate lyase in Candida lipolytica. J Bacteriol 144(2):692–697.
12. McKinlay JB, Vieille C, Zeikus JG. 2007. Prospects for a bio-based succinate industry. Appl Microbiol Biotechnol 76(4):727–740.
13. Okino S, Noburyu R, Suda M, Jojima T, Inui M, Yukawa H. 2008. An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl Microbiol Biotechnol 81(3):459–464.
14. Palmieri F, Agrimi G, Blanco E, Castegna A, Di Noia MA, Iacobazzi V, Lasorsa FM, Marobbio CM, Palmieri L, Scarcia P, Todisco S, Vozza A, Walker J. 2006. Identification of mitochondrial carriers in Saccharomyces cerevisiae by transport assay of reconstituted recombinant proteins. Biochim Biophys Acta 1757(9–10):1249–1262.
15. Pasteur ML. 1860. Memoire sur la fermentation alcoolique. Ann Chimie Physique 58(3rd serie):323–426.
16. Ratledge C. 2014. The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: A reappraisal and unsolved problems. Biotechnol Lett 36(8):1557–1568.
17. Rezaei MN, Aslankoohi E, Verstrepen KJ, Courtin CM. 2015. Contribution of the tricarboxylic acid (TCA) cycle and the glyoxylate shunt in Saccharomyces cerevisiae to succinic acid production during dough fermentation. Int J Food Microbiol 204:24–32.
18. Sato M, Nakahara T, Yamada K. 1972. Fermentative production of succinic acid from n-paraffin by Candida brumptii IFO 0731. Agric Biol Chem 36:1969–1974.
19. Sauer M, Porro D, Mattanovich D, Branduardi P. 2008. Microbial production of organic acids: Expanding the markets. Trends Biotechnol 26(2):100–108.
20. Taing O, Taing K. 2007. Production of malic and succinic acids by sugar-tolerant yeast Zygosaccharomyces rouxii. Eur Food Res Technol 224(3):343–347.
21. Vemuri GN, Eiteman MA, Altman E. 2002. Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. J Ind Microbiol Biotechnol 28(6):325–332.
22. Wasylenko TM, Ahn WS, Stephanopoulos G. 2015. The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica. Metab Eng 30:27–39.
23. Yuzbashev TV, Yuzbasheva EY, Laptev IA, Sobolevskaya TI, Vybornaya TV, Larina AS, Gvilava IT, Antonova SV, Sineoky SP. 2011. Is it possible to produce succinic acid at a low pH?. Bioeng Bugs 2(2):115–119.
24. Yuzbashev TV, Yuzbasheva EY, Sobolevskaya TI, Laptev IA, Vybornaya TV, Larina AS, Matsui K, Fukui K, Sineoky SP. 2010. Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica. Biotechnol Bioeng 107(4):673–682.