Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

Biotechnology and Bioengineering

Volumn 113, Issue 11, 2016, Pages 2342-2349

  Bolivar, Juan M ^a   Tribulato, Marco A ^a   Petrasek, Zdenek ^a   Nidetzky, Bernd ^a,b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

enzymatic oxidation; flow microreactor; glass; immobilization; microfluidics; silica binding module

Indexed keywords

AMINO ACIDS; BINS; BOROSILICATE GLASS; BUTYRIC ACID; CHEMICAL REACTORS; ENZYME ACTIVITY; ENZYMES; GLASS; ISOMERS; MASS TRANSFER; MICROCHANNELS; MICROFLUIDICS; PH EFFECTS; RADIOACTIVE WASTE VITRIFICATION; SILICA;

CONFOCAL LASER SCANNING MICROSCOPY; CONVENTIONAL REACTORS; D-AMINO ACID OXIDASE; ENZYMATIC OXIDATIONS; FLOW MICROREACTOR; HETEROGENEOUSLY CATALYZED REACTIONS; IMMOBILIZED ENZYME ACTIVITY; IN-LINE MEASUREMENTS;

ENZYME IMMOBILIZATION;

4 METHYLTHIO 2 OXOBUTYRIC ACID; CATALASE; DEXTRO AMINO ACID OXIDASE; DISSOLVED OXYGEN; GLASS; SILICON DIOXIDE;

ANALYTIC METHOD; ARTICLE; BIOCATALYSIS; BIOTRANSFORMATION; CONFOCAL LASER SCANNING MICROSCOPY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SUBSTRATE; HUMAN; IMMOBILIZED ENZYME REACTOR; MICROFLUIDICS; NONHUMAN; OXIDATION; PH; PUMP; REACTION DIFFUSION TIME SCALE ANALYSIS; SURFACE PROPERTY;

EID: 84988922896     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26011     Document Type: Article

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